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Biocalorimetry 2 [[electronic resource] ] : applications of calorimetry in the biological sciences / / edited by John E. Ladbury, Michael L. Doyle



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Titolo: Biocalorimetry 2 [[electronic resource] ] : applications of calorimetry in the biological sciences / / edited by John E. Ladbury, Michael L. Doyle Visualizza cluster
Pubblicazione: Chichester ; ; Hoboken, NJ, : Wiley, c2004
Edizione: 2nd ed.
Descrizione fisica: 1 online resource (277 p.)
Disciplina: 572.43
572.436
Soggetto topico: Calorimetry
Biology - Technique
Altri autori: LadburyJohn E. <1960->  
DoyleMichael L. <1959->  
Note generali: Rev. ed. of: Biocalorimetry.
Nota di bibliografia: Includes bibliographical references and index.
Nota di contenuto: Biocalorimetry 2; Contents; Preface; List of Contributors; Part I General Introduction; 1 Applications of Biocalorimetry: Binding, Stability and Enzyme Kinetics; 1.1 Introduction; 1.2 Principles of isothermal titration calorimetry (ITC); 1.3 Applications of ITC in the life sciences; 1.4 Thermodynamic signatures of non-covalent interactions; 1.5 Thermodynamic discrimination (TD); 1.6 ITC as a tool for studying drug-DNA interactions; 1.7 ITC as a tool for studying protein-DNA interactions; 1.8 The application of calorimetry for examining hydration effcts
1.9 The use of ITC for studying the kinetics and thermodynamics of enzyme catalysis1.10 Principles of differential scanning calorimetry (DSC); 1.11 Applications of DSC in the life sciences; 1.12 Thermodynamic stability; 1.13 Shelf life versus thermodynamic stability; 1.14 Specific and non-specific binding; 1.15 Intrinsic and extrinsic macromolecular stability; 1.16 Oligomerization; 1.17 The use of DSC for examining nucleic acid helix coil transitions; 1.18 Summary; Acknowledgements; References; Part II Isothermal Titration Calorimetry; 2 Isothermal Titration Calorimetry: A Tutorial
2.1 Introduction2.2 Thermodynamic characterization; 2.3 Instrumentation; 2.4 Raw data; 2.5 Basic considerations for experimental set-up; 2.6 Data analysis; 2.7 Summary; Application notes; Acknowledgement; References; 3 The Application of Isothermal Titration Calorimetry to Drug Discovery; 3.1 Introduction; 3.2 Overview of the drug discovery process; 3.3 Experimental measurement of thermodynamic binding parameters; 3.4 ITC in drug discovery; 3.5 Summary; References; 4 Dissecting the Thermodynamics of DNA-Protein Interactions; 4.1 Introduction; 4.2 Model systems
4.3 Comparison with the hydrophobic effect4.4 Protonation and charged-charged hydrogen bonds; 4.5 Dissection of the binding entropy; 4.6 Entropy contributions to the Sso7d-DNA interaction; 4.7 Entropy contributions to the GCN4-DNA interaction; 4.8 Discussion; Acknowledgements; References; 5 Salt Effects in Ribonuclease-Ligand Interactions: Screening or Competitive Binding?; 5.1 Introduction; 5.2 Anion binding to a protein-protein complex; 5.3 Charge-charge interactions in ribonuclease binding; 5.4 Conclusions; Acknowledgement; References
6 Thermodynamics-Structure Correlations of Sulfonamide Inhibitor Binding to Carbonic Anhydrase6.1 Introduction; 6.2 Identification of protonation reactions occurring upon binding; 6.3 Observed thermodynamics of inhibitor binding to CA; 6.4 Energetics of inhibitor protonation; 6.5 Sulfonamide 'anion' binding thermodynamics; 6.6 Correlations between structures and the thermodynamics of sulfonamide binding to CA; 6.7 Conclusions; References; 7 Energetics of the Interaction of Human Acidic Fibroblast Growth Factor with Heparin and the Functional Analogue Myo-Inositol Hexasulfate; 7.1 Introduction
7.2 Thermodynamic parameter derived from ITC experiments
Sommario/riassunto: Over the last decade, high-sensitivity calorimetry has developed from a specialist method used mainly by dedicated experts to a major, commercially available tool in the arsenal directed at understanding molecular interactions and stability. Calorimeters have now become commonplace in bioscience laboratories. As a result, the number of those proficient in experimentation in this field has risen dramatically, as has the range of experiments to which these methods have been applied. Applications extend from studies in small molecule and solvent biophysics, through drug screening to whole cell as
Titolo autorizzato: Biocalorimetry 2  Visualizza cluster
ISBN: 1-280-27608-8
9786610276080
0-470-01112-2
0-470-01111-4
Formato: Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione: Inglese
Record Nr.: 9910830141503321
Lo trovi qui: Univ. Federico II
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