Microbiology: Pearson New International Edition: A Laboratory Manual

• Autore: Cappuccino James
• Pubblicazione: [Place of publication not identified], : Pearson Education Limited, 2013
• Edizione: 10th ed.
• Descrizione fisica: 1 online resource (574 pages)
• Disciplina: 579.078
• Persona (resp. second.): ShermanNatalie
• Note generali: Bibliographic Level Mode of Issuance: Monograph
• Nota di contenuto: Cover -- Table of Contents -- Basic Laboratory Techniques for Isolation, Cultivation, and Cultural Characterization of Microorganisms -- 1. Effectiveness of Hand Washing -- 2. Culture Transfer Techniques -- 3. Techniques for Isolation of Pure Cultures -- 4. Cultural Characteristics of Microorganisms -- Microscopy -- 5. Microscopic Examination of Stained Cell Preparations -- 6. Microscopic Examination of Living Microorganisms Using a Hanging-Drop Preparation or a Wet Mount -- 7. The Microscopic Measurement of Microorganisms -- Bacterial Staining -- 8. Preparation of Bacterial Smears -- 9. Simple Staining -- 10. Negative Staining -- 11. Gram Stain -- 12. Acid-Fast Stain -- 13. Differential Staining for Visualization of Bacterial Cell Structures -- Cultivation of Microorganisms: Nutritional and Physical Requirements, and Enumeration of Microbial Populations -- 14. Nutritional Requirements: Media for the Routine Cultivation of Bacteria -- 15. Use of Differential, Selective, and Enriched Media -- 16. Physical Factors: Temperature -- 17. Physical Factors: pH of the Extracellular Environment -- 18. Physical Factors: Atmospheric Oxygen Requirements -- 19. Techniques for the Cultivation of Anaerobic Microorganisms -- 20. Serial Dilution - Agar Plate Procedure to Quantitate Viable Cells -- 21. The Bacterial Growth Curve -- Biochemical Activities of Microorganisms -- 22. Extracellular Enzymatic Activities of Microorganisms -- 23. Carbohydrate Fermentation -- 24. Triple Sugar-Iron Agar Test -- 25. IMViC Test -- 26. Hydrogen Sulfide Test -- 27. Urease Test -- 28. Litmus Milk Reactions -- 29. Nitrate Reduction Test -- 30. Catalase Test -- 31. Oxidase Test -- 32. Utilization of Amino Acids -- 33. Genus Identification of Unknown Bacterial Cultures -- The Protozoa -- 34. Free-Living Protozoa -- 35. Parasitic Protozoa -- The Fungi. ; 36. Cultivation and Morphology of Molds -- 37. Yeast Morphology, Cultural Characteristics, and Reproduction -- 38. Identification of Unknown Fungi -- The Viruses -- 39. Cultivation and Enumeration of Bacteriophages -- 40. Isolation of Coliphages from Raw Sewage -- Physical and Chemical Agents for the Control of Microbial Growth -- 41. Physical Agents of Control: Moist Heat -- 42. Physical Agents of Control: Environmental Osmotic Pressure -- 43. Physical Agents of Control: Electromagnetic Radiations -- 44. Chemical Agents of Control: Chemotherapeutic Agents -- 45. Determination of Penicillin Activity in the Presence and Absence of Penicillinase -- 46. Chemical Agents of Control: Disinfectants and Antiseptics -- Microbiology of Food -- 47. Microbiological Analysis of Food Products: Bacterial Count -- 48. Wine Production -- Microbiology of Water -- 49. Standard Qualitative Analysis of Water -- 50. Quantitative Analysis of Water: Membrane Filter Method -- Microbiology of Soil -- 51. Microbial Populations in Soil: Enumeration -- 52. Isolation of Antibiotic-Producing Microorganisms and Determination of Antimicrobial Spectrum of Isolates -- 53. Isolation of Pseudomonas Species by Means of the Enrichment Culture Technique -- Bacterial Genetics -- 54. Enzyme Induction -- 55. Bacterial Conjugation -- 56. Isolation of a Streptomycin-Resistant Mutant -- 57. The Ames Test: A Bacterial Test System for Chemical Carcinogenicity -- Biotechnology -- 58. Bacterial Transformation -- 59. Isolation of Bacterial Plasmids -- 60. Restriction Analysis and Electrophoretic Separation of Bacteriophage Lambda DNA -- Medical Microbiology -- 61. Microbial Flora of the Mouth: Determination of Susceptibility to Dental Caries -- 62. Normal Microbial Flora of the Throat and Skin -- 63. Identification of Human Staphylococcal Pathogens. ; 64. Identification of Human Streptococcal Pathogens -- 65. Identification of Streptococcus pneumoniae -- 66. Identification of Enteric Microorganisms Using Computer-Assisted Multitest Microsystems -- 67. Isolation and Presumptive Identification of Campylobacter -- 68. Microbiological Analysis of Blood Specimens -- 69. Microbiological Analysis of Urine Specimens -- 70. Species Identification of Unknown Bacterial Cultures -- Immunology -- 71. Precipitin Reaction: The Ring Test -- 72. Agglutination Reaction: The Febrile Antibody -- 73. Immunofluorescence -- 74. Enzyme-Linked Immunoabsorbent Assay -- 75. Sexually Transmitted Diseases: Rapid Immunodiagnostic Procedures -- Scientific Notation -- Methods for the Preparation of Dilutions -- Microbiological Media -- Biochemical Test Reagents -- Staining Reagents -- Experimental Microorganisms -- Index.
• Sommario/riassunto: Versatile, comprehensive, and clearly written, this competitively priced laboratory manual can be used with any undergraduate microbiology text-and now features brief clinical applications for each experiment, MasteringMicrobiology® quizzes that correspond to each experiment, and a new experiment on hand washing. Microbiology: A Laboratory Manual is known for its thorough coverage, descriptive and straightforward procedures, and minimal equipment requirements. A broad range of experiments helps to convey basic principles and techniques. Each experiment includes an overview, an in-depth discussion of the principle involved, easy-to-follow procedures, and lab reports with review and critical thinking questions. Ample introductory material and laboratory safety instructions are provided.
• Altri titoli varianti: Microbiology
• Titolo autorizzato: Microbiology: Pearson New International Edition: A Laboratory Manual
• ISBN: 1-292-05223-6
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Lingua di pubblicazione: Inglese
• Record Nr.: 9910153087403321
• Lo trovi qui: Univ. Federico II