Light scattering, size exclusion chromatography, and asymmetric flow field flow fractionation [[electronic resource] ] : powerful tools for the characterization of polymers, proteins, and nanoparticles / / Stepan Podzimek

• Autore: Podzimek Stepan <1955->
• Pubblicazione: Hoboken, NJ, : Wiley, c2011
• Descrizione fisica: 1 online resource (373 p.)
• Disciplina: 543/.8; 547.7
• Soggetto topico: Polymers - Analysis; Polymers - Separation; Light - Scattering; Chromatographic analysis
• Note generali: Description based upon print version of record.
• Nota di bibliografia: Includes bibliographical references and index.
• Nota di contenuto: Light Scattering, Size Exclusion Chromatography and Asymmetric Flow Field Flow Fractionation; Contents; Preface; 1 Polymers; 1.1 Introduction; 1.2 Molecular Structure of Polymers; 1.2.1 Macromolecules in Dilute Solution; 1.3 Molar Mass Distribution; 1.3.1 Description of Molar Mass Distribution; 1.3.1.1 Distribution Functions; 1.3.1.2 Molar Mass Averages; 1.4 Methods for the Determination of Molar Mass; 1.4.1 Method of End Groups; 1.4.2 Osmometry; 1.4.2.1 Vapor Pressure Osmometry; 1.4.2.2 Membrane Osmometry; 1.4.3 Dilute Solution Viscometry; 1.4.3.1 Properties of Mark-Houwink Exponent ; 1.4.3.2 Molecular Size from Intrinsic Viscosity1.4.3.3 Dependence of Intrinsic Viscosity on Polymer Structure, Temperature, and Solvent; 1.4.4 Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry; 1.4.5 Analytical Ultracentrifugation; 1.5 Keynotes; 1.6 References; 2 Light Scattering; 2.1 Theory and Basic Principles; 2.2 Types of Light Scattering; 2.2.1 Static Light Scattering; 2.2.1.1 Particle Scattering Functions; 2.2.1.2 Light Scattering Formalisms; 2.2.1.3 Processing the Experimental Data; 2.2.2 Dynamic Light Scattering; 2.3 Light Scattering Instrumentation ; 2.4 Specific Refractive Index Increment2.5 Light Scattering in Batch and Chromatography Mode; 2.6 Parameters Affecting Accuracy of Molar Mass Determined by Light Scattering; 2.7 Examples of Light Scattering Measurement in Batch Mode; 2.8 Keynotes; 2.9 References; 3 Size Exclusion Chromatography; 3.1 Introduction; 3.2 Separation Mechanisms; 3.2.1 Steric Exclusion; 3.2.2 Restricted Diffusion; 3.2.3 Separation by Flow; 3.2.4 Peak Broadening and Separation Efficiency; 3.2.5 Secondary Separation Mechanisms; 3.3 Instrumentation; 3.3.1 Solvents; 3.3.2 Columns and Column Packing; 3.3.3 Detectors ; 3.3.3.1 UV Detector3.3.3.2 Refractive Index Detector; 3.3.3.3 Infrared Detector; 3.3.3.4 Evaporative Light Scattering Detector; 3.3.3.5 Viscosity Detector; 3.3.3.6 Light Scattering Detector; 3.3.3.7 Other Types of Detectors; 3.4 Column Calibration; 3.4.1 Universal Calibration; 3.4.2 Flow Marker; 3.5 SEC Measurements and Data Processing; 3.5.1 Sample Preparation; 3.5.2 Determination of Molar Mass and Molar Mass Distribution; 3.5.3 Reporting Results; 3.5.4 Characterization of Chemical Composition of Copolymers and Polymer Blends; 3.5.5 Characterization of Oligomers ; 3.5.6 Influence of Separation Conditions3.5.7 Accuracy, Repeatability, and Reproducibility of SEC Measurements; 3.6 Applications of SEC; 3.7 Keynotes; 3.8 References; 4 Combination of SEC and Light Scattering; 4.1 Introduction; 4.2 Data Collection and Processing; 4.2.1 Processing MALS Data; 4.2.1.1 Debye Fit Method; 4.2.1.2 Zimm Fit Method; 4.2.1.3 Berry Fit Method; 4.2.1.4 Random Coil Fit Method; 4.2.1.5 Influence of Light Scattering Formalism on Molar Mass and RMS Radius; 4.2.2 Determination of Molar Mass and RMS Radius Averages and Distributions; 4.2.3 Chromatogram Processing ; 4.2.4 Influence of Concentration and Second Virial Coefficient
• Sommario/riassunto: A comprehensive, practical approach to three powerful methods of polymer analysis and characterization This book serves as a complete compendium of three important methods widely used for the characterization of synthetic and natural polymers-light scattering, size exclusion chromatography (SEC), and asymmetric flow field flow fractionation (A4F). Featuring numerous up-to-date examples of experimental results obtained by light scattering, SEC, and A4F measurements, Light Scattering, Size Exclusion Chromatography and Asymmetric Flow Field Flow Fractionation takes an all-in-one approach to
• Titolo autorizzato: Light scattering, size exclusion chromatography, and asymmetric flow field flow fractionation
• ISBN: 1-118-10272-X; 1-283-02506-X; 9786613025067; 0-470-87797-9; 0-470-87796-0
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Lingua di pubblicazione: Inglese
• Record Nr.: 9910139214603321
• Lo trovi qui: Univ. Federico II