Human molecular biology laboratory manual [[electronic resource] /] / Stefan Surzycki

• Autore: Surzycki Stefan <1936->
• Pubblicazione: Malden, MA, : Blackwell Pub., 2003
• Descrizione fisica: 1 online resource (246 p.)
• Disciplina: 611.01816; 611/.01816
• Soggetto topico: Molecular biology; Human genetics
• Note generali: Description based upon print version of record.
• Nota di bibliografia: Includes bibliographical references and index.
• Nota di contenuto: Human Molecular Biology Laboratory; Contents; Preface; Chapter 1 Preparation of Human Genomic DNA; Introduction; Background; Cell Breakage; Removal of Protein; Deproteinization using organic solvents; Deproteinization using enzymes; Removal of RNA; Concentrating the DNA; Determination of the Purity and Quantity of DNA; First Laboratory Period; Safety precautions; Technical tips; Protocol; Second Laboratory Period; Safety precautions; Protocol; Technical tips; Expected results; References; Chapter 2 DNA Fingerprinting: Multi-locus Analysis; Introduction; Background; First Laboratory Period ; Experiment 1: Restriction Enzyme DigestionIntroduction; Background; Technical tips; Protocol; References; Experiment 2: agarose gel electrophoresis; Introduction; Background; Safety precautions; Protocol; References; Second Laboratory Period; Experiment 3: Southern blotting; Introduction; Background; Safety precautions; Technical tips; Protocol; References; Third Laboratory Period; Experiment 4: preparation of probe and hybridization; Introduction; Background; Technical tips; Protocol; References; Fourth Laboratory Period; Protocol; Fifth Laboratory Period; Protocol; Data analysis ; Chapter 3 DNA Fingerprinting: Single-locus AnalysisIntroduction; Background; First Laboratory Period; Protocol; Second Laboratory Period; Protocol; Data analysis; Expected results; References; General reading; Chapter 4 Out of Africa: Origin of Modern Humans; Introduction; Background; Origin of humans; PCR; First Laboratory Period; Technical tips; Protocol; Second Laboratory Period; Safety precautions; Protocol; Data analysis; Expected results; References; Chapter 5 DNA Sequencing; Introduction; Background; DNA sequencing methods; Sequencing strategies; References; First Laboratory Period ; Experiment 1: nebulization shearing of DNAIntroduction; Background; Safety precautions; Technical tips; Protocol; Expected results; Experiment 2: repair of the ends of sheared DNA; Introduction; Safety precautions; Technical tips; Protocol; References; Second Laboratory Period; Experiment 3: ligation to sequencing vector; Introduction; Background; Technical tips; Protocol; References; Experiment 4: transformation of bacteria by electroporation; Introduction; Background; Technical tips; Protocol; Expected results; References; Third Laboratory Period ; Experiment 5: preparation of plasmid for DNA sequencingIntroduction; Background; Technical tips; Protocol; Experiment 6: sequencing reactions for an ABI 3700 sequencer; Introduction; Technical tips; Protocol; Fourth Laboratory Period; Experiment 7: removing dideoxy terminators; Protocol; References; Chapter 6 Computer Analysis of Sequencing Data; Introduction; Background; Databases and sequence formats; Sequence alignments; BLAST; FASTA; BLAST versus FASTA; Single-sequence analysis; Dot matrix analysis; Technical tips; Protocol; References; Sequence Alignment with BLAST ; Search of ""nr"" database
• Sommario/riassunto: Human Molecular Biology Laboratory Manual offers a hands-on, state-of-the-art introduction to modern molecular biology techniques as applied to human genome analysis. In eight unique experiments, simple step-by-step instructions guide students through the basic principles of molecular biology and the latest laboratory techniques. This laboratory manual's distinctive focus on human molecular biology provides students with the opportunity to analyze and study their own genes while gaining real laboratory experience. A Background section highlighting the theoretical princip
• Titolo autorizzato: Human molecular biology laboratory manual
• ISBN: 1-281-31269-X; 9786611312695; 0-470-99925-X; 0-470-99924-1
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Lingua di pubblicazione: Inglese
• Record Nr.: 996205987903316
• Lo trovi qui: Univ. di Salerno