Bioluminescence: Fundamentals and Applications in Biotechnology - Volume 2 / / edited by Gérald Thouand, Robert Marks

• Pubblicazione: Berlin, Heidelberg : , : Springer Berlin Heidelberg : , : Imprint : Springer, , 2014
• Edizione: 1st ed. 2014.
• Descrizione fisica: 1 online resource (VI, 224 p. 40 illus., 19 illus. in color.) : online resource
• Disciplina: 572.4358
• Soggetto topico: Microbiology; Applied Microbiology
• Persona (resp. second.): ThouandGérald; MarksRobert (Robert S.)
• Note generali: Includes index.
• Nota di contenuto: Intro -- Contents -- Part I Fundamentals of Bioluminescence -- 1 How Synthetic Biology Will Reconsider Natural Bioluminescence and Its Applications -- Abstract -- 1...Introduction -- 2...Applications -- 2.1 Illumination -- 2.2 Aesthetics -- 2.3 Communication with Humans -- 2.4 Communication with Other Engineered Systems -- 3...Feasibility of Bio-lighting -- 3.1 Photosynthetic Efficiency -- 3.2 Energy Output -- 3.3 Competing with Conventional Lighting -- 4...Bioluminescent Parts and Modules in Nature -- 4.1 Firefly Luminescence -- 4.2 Bacterial Bioluminescence -- 4.3 Other Natural Luminescence Systems -- 5...Selecting Chassis Organisms -- 6...Controlling Luminescence -- 7...Containment -- 8...Improving and Altering Luminescence -- 8.1 Alterations -- 9...Conclusions -- References -- 2 Optimization of the Firefly Luciferase Reaction for Analytical Purposes -- Abstract -- 1...Introduction -- 2...Pretreatment of Samples -- 2.1 Concentration of Bacteria -- 2.2 Dilution -- 2.3 Pretreatment to Remove Specific Pools of ATP -- 2.4 Resuscitation -- 3...Extraction of Intracellular ATP -- 4...Selection of Luciferase for Different Applications -- 4.1 Reporter Gene Assays -- 4.2 ATP Assays -- 5...Luciferin -- 5.1 d-Luciferin -- 5.2 Luciferin Analogues and Derivatives -- 6...Kinetics of the Luciferase Reaction -- 7...Optimization of Reagents -- 7.1 Optimization of Assays of ATP -- 7.2 Optimization of Assays of Luciferase -- 7.3 Optimization of Coupled Enzyme Assays -- 7.3.1 Endpoint Assays of Metabolites -- 7.3.2 Kinetic Assays of Metabolites -- 7.3.3 Kinetic Assays of ATP-Forming Enzymes -- 7.3.4 Kinetic Assays of ATP-Depleting Enzymes -- 8...Stabilization of Reagents -- 9...Calibration of Assays -- 10...Sources of Background and Ways to Reduce Them -- 11...Effect of Measuring Different Light Parameters/Read-Outs -- 12...Conclusions -- Acknowledgements -- References. ; Part II Applications of Bioluminescencein Environment and Security -- 3 Evaluation of the Ecotoxicity of Pollutants with Bioluminescent Microorganisms -- Abstract -- 1...Introduction -- 2...Naturally Bioluminescent Microorganisms: Their Use in Environmental Monitoring -- 3...Recombinant Bioluminescent Microorganisms: Their Use in Environmental Monitoring -- 3.1 Specific Soil Bioreporters -- 3.2 Bioreporters Specially Designed to Assess Bioremediation Programs and Wastewater Toxicity Evaluation -- 3.3 Freshwater Bioreporters -- 3.4 Eukaryotic Bioreporters -- 3.5 Aequorin-Based Bioreporters -- 4...Relevance of QSAR Models and Hormesis in Ecotoxicology -- 4.1 Bioluminescent Microorganisms and QSAR Models -- 4.2 Hormetic Response of Bioluminescent Organisms and Its Implications in Ecotoxicity Testing -- 5...Use of Bioluminescent Microorganisms to Assess Mixture Toxicity: From Whole Toxicity Determination to Development of Conceptual Models to Study the Toxicity of Complex Mixtures of Pollutants -- 5.1 Whole Toxicity Assessment -- 5.2 Bioluminescent Microorganisms and Toxicology of Mixtures -- 6...Concluding Remarks -- References -- 4 Molecular Manipulations for Enhancing Luminescent Bioreporters Performance in the Detection of Toxic Chemicals -- Abstract -- Abstract -- 1...Introduction -- 2...Genetic Manipulations of the Host Strain and Expression System -- 3...Genetic Manipulations of the Sensing Element -- 4...Genetic Manipulations of lux Reporting Elements -- 5...Additional Genetic Manipulations and the Advent of Synthetic Biology -- 6...Summary -- References -- Part III Applications of Bioluminescencein Agriculture and Bioprocess -- 5 Biosensors, Antibiotics and Food -- Abstract -- 1...Introduction -- 2...Antibiotic Use and Associated Risks -- 3...Control of Antibiotic Use -- 4...Detection Methods of Antibiotic Residues in Food -- 4.1 Confirmatory Analysis of Antibiotic Residues. ; 4.2 Screening Methods for Antibiotic Residues -- 5...Biosensors for Detection of Antibiotics in Food -- 5.1 Whole-Cell Biosensors -- 5.2 Inducible Whole-Cell Biosensors for Antibiotic Detection -- 6...Conclusions -- References -- Part IV Applications of Bioluminescencein Health -- 6 Rapid In-vitro Testing for Chemotherapy Sensitivity in Leukaemia Patients -- Abstract -- 1...Introduction: Bioluminescent Biosensors for Drug Sensitivity -- 1.1 Bioluminescent Reporter Technology -- 1.2 Real-Time Monitoring of Anti-microbial Effects -- 1.3 Intracellular Anti-microbial Effects -- 2...The Clinical Need in Leukaemia -- 3...Construction of a Bacterial Biosensor for Chemotherapy Sensitivity Testing -- 3.1 Mode of Action of Target Drug -- 3.2 Construction of Cytarabine Sensitive E. Coli Host -- 3.3 Bioluminescent Biosensor Development -- 4...Validation of the Biosensor Assay EA -- 4.1 Validation of Ara-CTP Measurement -- 4.2 Assay Sensitivity and Specificity -- 4.3 Assay Reproducibility and Stability -- 5...Clinical Testing -- 6...Future Perspectives -- 6.1 Multi Drug Testing -- 6.2 Cytarabine Biosensor -- 6.3 Applications with Other Drugs -- 6.4 Additional Chemotherapy Biosensors -- 6.5 Rapid Testing in Other Cancers -- References -- Index.
• Sommario/riassunto: This book review series presents current trends in modern biotechnology. The aim is to cover all aspects of this interdisciplinary technology where knowledge, methods and expertise are required from chemistry, biochemistry, microbiology, genetics, chemical engineering and computer science. Volumes are organized topically and provide a comprehensive discussion of developments in the respective field over the past 3-5 years. The series also discusses new discoveries and applications. Special volumes are dedicated to selected topics which focus on new biotechnological products and new processes for their synthesis and purification. In general, special volumes are edited by well-known guest editors. The series editor and publisher will however always be pleased to receive suggestions and supplementary information. Manuscripts are accepted in English.
• Titolo autorizzato: Bioluminescence: Fundamentals and Applications in Biotechnology - Volume 2
• ISBN: 3-662-43619-1
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Lingua di pubblicazione: Inglese
• Record Nr.: 9910298647303321
• Lo trovi qui: Univ. Federico II
• Serie: Advances in Biochemical Engineering/Biotechnology, . 0724-6145 ; ; 145