Practical biotransformations [[electronic resource] ] : a beginner's guide / / Gideon Grogan

• Autore: Grogan Gideon
• Pubblicazione: Chichester, West Sussex, : Wiley, 2009
• Descrizione fisica: 1 online resource (353 p.)
• Disciplina: 660.6
• Soggetto topico: Biosynthesis; Enzymes - Biotechnology; Microbial biotechnology; Organic compounds - Synthesis
• Note generali: Description based upon print version of record.
• Nota di bibliografia: Includes bibliographical references and index.
• Nota di contenuto: Practical Biotransformations; Contents; Foreword; Preface; 1 Biotransformations, Microbes and Enzymes; 1.1 Introduction; 1.2 Biotransformations; 1.3 Microorganisms; 1.4 Organism Nomenclature; 1.5 Enzymes; 1.6 Types of Enzymatic Reactions; 1.7 Enzymatic Cofactors; 1.8 Some Basic Characteristics of Enzyme Catalysis; 1.9 Types of Biocatalyst - Biotransformations by 'Whole Cells' or 'Isolated Enzymes'; 1.10 Conclusion; References; 2 An Overview of Biocatalyst Sources and Web-Based Information; 2.1 Introduction; 2.2 Microbial Culture Collections; 2.3 Obtaining Organisms from Other Research Groups ; 2.4 Selective Enrichments 2.5 Metagenomics; 2.6 Enzyme Suppliers and Biocatalyst Development Companies; 2.7 Genome Mining for Biocatalysts; 2.8 Obtaining Amino Acid and Gene Sequence Information on Biocatalysts; 2.9 Obtaining DNA Templates for Cloning; 2.10 Custom Gene Synthesis; 2.11 Other Web Resources for Finding Information on Biocatalysts; 2.12 Conclusion; References; 3 Setting up a Laboratory for Biotransformations; 3.1 Introduction; 3.2 Microbiological Containment; 3.3 On Containment Issues and Genetically Modified Organisms (GMOs); 3.4 Equipment for Handling Microorganisms ; 3.5 Techniques and Terms in Microbiology - Sterility, Asepsis and Aseptic Technique 3.6 Disposal of Viable Microbial Waste and Disinfection of Reusable Equipment; 3.7 Equipment for Enzymology and Molecular Biology; 3.8 General Reagents and Chemicals in a Biotransformations Laboratory; 3.9 Conclusion; References; 4 A Beginner's Guide to Preparative Whole-Cell Microbial Transformations; 4.1 Introduction; 4.2 Storage, Maintenance and Growth of Microorganisms; 4.3 General Microbiological Methods; 4.4 Examples of Whole-Cell Biotransformations Using Bacteria ; 4.5 Biotransformation by Filamentous Fungi and Yeasts 4.6 Whole-Cell Biotransformations by Recombinant Strains of E. Coli; 4.7 Conclusion; References; 5 A Beginner's Guide to Biotransformations Catalysed by Commercially Available Isolated Enzymes; 5.1 Introduction; 5.2 Lipases; 5.3 Hydrolytic Reactions Using Lipases; 5.4 Using Lipases for Acylation Reactions; 5.5 Other Hydrolases; 5.6 Commercially Available Coenzyme-Dependent Enzymes; 5.7 Carbon-Carbon Bond Forming Reactions; 5.8 Conclusion; References; 6 A Beginner's Guide to the Isolation, Analysis and Use of Home-Grown Enzyme Biocatalysts ; 6.1 Introduction 6.2 Cell Growth and Harvesting; 6.3 Cell Disruption; 6.4 A Typical Procedure for Making a Cell Extract from a Recombinant Strain of E. Coli; 6.5 Purification of Enzymes - A Brief Guide; 6.6 Techniques for Protein Purification; 6.7 Isolation of Recombinant Enzymes Using Histidine Tags; 6.8 Estimation of Protein Concentration; 6.9 Concentrating Protein Samples by Centrifugation; 6.10 Analysis of Protein Samples by Sodium Dodecylsulfate Polyacylamide Gel Electrophoresis; 6.11 Examples of Enzyme Assays; 6.12 Using Home-Grown Enzymes for Biotransformations - Some Recent Examples ; 6.13 Conclusion
• Sommario/riassunto: The development of new asymmetric catalytic methods is of fundamental importance to industrial synthetic chemistry. The demand for optically pure synthetic intermediates and the drive to adopt greener methods of synthesis have stimulated a growing interest in biocatalysis as a selective and environmentally benign synthetic technique. Practical Biotransformations: A Beginner's Guide provides an introduction to microbes and enzymes and demonstrates their practical applications in synthetic organic chemistry. Designed as a laboratory manual, this user-friendly guide discusses standard la
• Titolo autorizzato: Practical biotransformations
• ISBN: 1-4051-7125-1; 1-282-12408-0; 9786612124082; 1-4051-9368-9
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Lingua di pubblicazione: Inglese
• Record Nr.: 9910780898303321
• Lo trovi qui: Univ. Federico II
• Serie: Postgraduate chemistry series.