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Autore: |
Frank J (Joachim), <1940->
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Titolo: |
Three-dimensional electron microscopy of macromolecular assemblies : visualization of biological molecules in their native state / / Joachim Frank
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Pubblicazione: | Oxford ; ; New York, : Oxford University Press, 2006 |
Edizione: | 2nd ed. |
Descrizione fisica: | 1 online resource (427 p.) |
Disciplina: | 570/.28/25 |
Soggetto topico: | Three-dimensional imaging in biology |
Electron microscopy | |
Note generali: | Description based upon print version of record. |
Nota di bibliografia: | Includes bibliographical references (p. 345-398) and index. |
Nota di contenuto: | Contents; CHAPTER 1 Introduction; 1 The Electron Microscope and Biology; 1.1 General Remarks; 1.2 Three-Dimensional Electron Microscopy; 2 Single-Particle Versus Crystallographic Analysis; 3 Crystallography without Crystals; 4 Toward a Unified Approach to Structural Analysis of Macromolecules; 5 Single-Particle Reconstruction, Macromolecular Machines, and Structural Proteomics; 6 The Electron Microscope and the Computer; CHAPTER 2 Electron Microscopy of Macromolecular Assemblies; 1 Principle of the Transmission Electron Microscope; 2 Specimen Preparation Methods; 2.1 Introduction |
2.2 Negative Staining2.3 Glucose Embedment; 2.4 Use of Tannic Acid; 2.5 Ice-Embedded Specimens; 2.6 Hybrid Techniques: Cryo-Negative Staining; 2.7 Labeling with Gold Clusters; 2.8 Support Grids; 3 Principle of Image Formation in the Transmission Electron Microscope; 3.1 Introduction; 3.2 The Weak-Phase Object Approximation; 3.3 The Contrast Transfer Theory; 3.4 Amplitude Contrast; 3.5 Formulation of Bright-Field Image Formation Using Complex Atomic Scattering Amplitudes; 3.6 Optical and Computational Diffraction Analysis-The Power Spectrum; 3.7 Determination of the Contrast Transfer Function | |
3.8 Instrumental Correction of the Contrast Transfer Function3.9 Computational Correction of the Contrast Transfer Function; 3.10 Locally Varying CTF and Image Quality; 4 Special Imaging Techniques and Devices; 4.1 Low-Dose Electron Microscopy; 4.2 Spot Scanning; 4.3 Energy Filtration; 4.4 Direct Image Readout and Automated Data Collection; CHAPTER 3 Two-Dimensional Averaging Techniques; 1 Introduction; 1.1 The Different Sources and Types of Noise; 1.2 Principle of Averaging: Historical Notes; 1.3 Equivalence between Averaging and Quasi-Optical Fourier Filtration | |
1.4 A Discourse on Terminology: Views Versus Projections1.5 The Role of Two-Dimensional Averaging in the Three-Dimensional Analysis of Single Molecules; 1.6 Origins of Orientational Preferences; 2 Digitization and Selection of Particles; 2.1 Hardware for Digitization; 2.2 The Sampling Theorem; 2.3 Interactive Particle Selection; 2.4 Automated Particle Selection; 3 Alignment Methods; 3.1 Quantitative Definitions of Alignment; 3.2 Homogeneous Versus Heterogeneous Image Sets; 3.3 Translational and Rotational Cross-Correlation; 3.4 Reference-Based Alignment Techniques | |
3.5 Reference-Free Alignment Techniques3.6 Alignment Using the Radon Transform; 4 Averaging and Global Variance Analysis; 4.1 The Statistics of Averaging; 4.2 The Variance Map and the Analysis of Statistical Significance; 4.3 Signal-to-Noise Ratio; 5 Resolution; 5.1 The Concept of Resolution; 5.2 Resolution Criteria; 5.3 Resolution and Cross-Resolution; 5.4 Resolution-Limiting Factors; 5.5 Statistical Requirements following the Physics of Scattering; 5.6 Noise Filtering; 6 Validation of the Average Image; CHAPTER 4 Multivariate Data Analysis and Classification of Images; 1 Introduction | |
1.1 Heterogeneity of Image Sets | |
Sommario/riassunto: | 1. Introduction2. Electron Microscopy of Macromolecular Assemblies3. Two-Dimensional Averaging Techniques4. Multivariate Data Analysis and Classification of Images5. Three-Dimensional Reconstruction6. Interpretation of Three-Dimensional Images of MacromoleculesAppendix 1: Some Important Definitions and TheoremsAppendix 2: Profiles, Point-Spread Functions, and Effects of Commonly Used Low-Pass FiltersAppendix 2: Bibliography of MethodsAppendix 2: Bibliography of StructuresAppendix 2: Special Journal Issues on Image Processing Techniques |
Titolo autorizzato: | Three-dimensional electron microscopy of macromolecular assemblies ![]() |
ISBN: | 0-19-029288-1 |
0-19-803438-5 | |
1-4294-1521-5 | |
0-19-515096-1 | |
1-280-84551-1 | |
Formato: | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione: | Inglese |
Record Nr.: | 9910820080403321 |
Lo trovi qui: | Univ. Federico II |
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