Enzymes [[electronic resource] ] : a practical introduction to structure, mechanism, and data analysis / / Robert A. Copeland

• Autore: Copeland Robert Allen
• Pubblicazione: New York, : J. Wiley, c2014
• Edizione: 2nd ed.
• Descrizione fisica: 1 online resource (417 p.)
• Disciplina: 572.7
• Soggetto topico: Enzymes; Enzymology
• Note generali: "Published simultaneously in Canada."
• Nota di bibliografia: Includes bibliographical references and index.
• Nota di contenuto: ENZYMES SECOND EDITION; CONTENTS; Preface; Acknowledgments; Preface to the First Edition; 1 A Brief History of Enzymology; 1.1 Enzymes in Antiquity; 1.2 Early Enzymology; 1.3 The Development of Mechanistic Enzymology; 1.4 Studies of Enzyme Structure; 1.5 Enzymology Today; 1.6 Summary; References and Further Reading; 2 Chemical Bonds and Reactions in Biochemistry; 2.1 Atomic and Molecular Orbitals; 2.2 Thermodynamics of Chemical Reactions; 2.3 Acid-Base Chemistry; 2.4 Noncovalent Interactions in Reversible Binding; 2.5 Rates of Chemical Reactions; 2.6 Summary; References and Further Reading ; 3 Structural Components of Enzymes3.1 The Amino Acids; 3.2 The Peptide Bond; 3.3 Amino Acid Sequence or Primary Structure; 3.4 Secondary Structure; 3.5 Tertiary Structure; 3.6 Subunits and Quaternary Structure; 3.7 Cofactors in Enzymes; 3.8 Summary; References and Further Reading; 4 Protein-Ligand Binding Equilibria; 4.1 The Equilibrium Dissociation Constant, K(d); 4.2 The Kinetic Approach to Equilibrium; 4.3 Binding Measurements at Equilibrium; 4.4 Graphic Analysis of Equilibrium Ligand Binding Data; 4.5 Equilibrium Binding with Ligand Depletion (Tight Binding Interactions) ; 4.6 Competition Among Ligands for a Common Binding Site4.7 Experimental Methods for Measuring Ligand Binding; 4.8 Summary; References and Further Reading; 5 Kinetics of Single-Substrate Enzyme Reactions; 5.1 The Time Course of Enzymatic Reactions; 5.2 Effects of Substrate Concentration on Velocity; 5.3 The Rapid Equilibrium Model of Enzyme Kinetics; 5.4 The Steady State Model of Enzyme Kinetics; 5.5 The Significance of k(cat) and K(m); 5.6 Experimental Measurement of k(cat) and K(m); 5.7 Other Linear Transformations of Enzyme Kinetic Data; 5.8 Measurements at Low Substrate Concentrations ; 5.9 Deviations from Hyperbolic Kinetics5.10 Transient State Kinetic Measurements; 5.11 Summary; References and Further Reading; 6 Chemical Mechanisms in Enzyme Catalysis; 6.1 Substrate-Active Site Complementarity; 6.2 Rate Enhancement Through Transition State Stabilization; 6.3 Chemical Mechanisms for Transition State Stabilization; 6.4 The Serine Proteases: An Illustrative Example; 6.5 Enzymatic Reaction Nomenclature; 6.6 Summary; References and Further Reading; 7 Experimental Measures of Enzyme Activity; 7.1 Initial Velocity Measurements; 7.2 Detection Methods ; 7.3 Separation Methods in Enzyme Assays7.4 Factors Affecting the Velocity of Enzymatic Reactions; 7.5 Reporting Enzyme Activity Data; 7.6 Enzyme Stability; 7.7 Summary; References and Further Reading; 8 Reversible Inhibitors; 8.1 Equilibrium Treatment of Reversible Inhibition; 8.2 Modes of Reversible Inhibition; 8.3 Graphic Determination of Inhibitor Type; 8.4 Dose-Response Curves of Enzyme Inhibition; 8.5 Mutually Exclusive Binding of Two Inhibitors; 8.6 Structure-Activity Relationships and Inhibitor Design; 8.7 Summary; References and Further Reading; 9 Tight Binding Inhibitors ; 9.1 Identifying Tight Binding Inhibition
• Sommario/riassunto: Fully updated and expanded-a solid foundation for understanding experimental enzymology. This practical, up-to-date survey is designed for a broad spectrum of biological and chemical scientists who are beginning to delve into modern enzymology. Enzymes, Second Edition explains the structural complexities of proteins and enzymes and the mechanisms by which enzymes perform their catalytic functions. The book provides illustrative examples from the contemporary literature to guide the reader through concepts and data analysis procedures. Clear, well-written descriptions sim
• Titolo autorizzato: Enzymes
• ISBN: 1-280-36703-2; 9786610367030; 0-470-23916-6; 0-471-46185-7; 0-471-22063-9
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Lingua di pubblicazione: Inglese
• Record Nr.: 9910677846903321
• Lo trovi qui: Univ. Federico II