Flow cytometry [[electronic resource] ] : first principles / / Alice Longobardi Givan

• Autore: Givan Alice Longobardi
• Pubblicazione: New York, : Wiley-Liss, c2001
• Edizione: 2nd ed.
• Descrizione fisica: 1 online resource (292 p.)
• Disciplina: 571.6/028; 571.60282
• Soggetto topico: Flow cytometry; Cytology
• Note generali: Description based upon print version of record.
• Nota di bibliografia: Includes bibliographical references (p. 229-231) and index.
• Nota di contenuto: Contents; Preface; Acknowledgments (First Edition); Acknowledgments (Second Edition); 1 The Past as Prologue; 2 Setting the Scene; 3 Instrumentation: Into the Black Box; Illumination of the Stream; Centering Cells in the Illuminating Beam; Detection of Signals from Cells; Electronics; 4 Information: Harnessing the Data; Data Storage; Data Analysis; 5 Seeing the Light: Lasers, Fluorochromes, and Filters; General Theory; Lasers; Fluorochromes; Partitioning the Signal with Lenses, Filters, and Mirrors; Spectral Compensation ; 6 Cells from Without: Leukocytes, Surface Proteins, and the Strategy of GatingCells from Blood; Staining for Surface Markers; Controls; Quantitation; Sensitivity; The Strategy of Gating; Gating on Fluorescence; 7 Cells from Within: Intracellular Proteins; Methods for Permeabilizing Cells; Examples of Intracellular Staining; 8 Cells from Within: DNA in Life and Death; Fluorochromes for DNA Analysis; Ploidy; Cell Cycle Analysis; Two-Color Analysis for DNA and Another Parameter; Chromosomes; Apoptosis; Necrosis; 9 The Sorting of Cells; Sorting Theory; Characterization of Sorted Cells ; Alternative Methods for SortingThe Condition of Cells After Sorting; 10 Disease and Diagnosis: The Clinical Laboratory; The Hematology Laboratory; The Pathology Laboratory; Solid Organ Transplantation; Comments; 11 Out of the Mainstream: Research Frontiers; Functional Assays; The Aquatic Environment; Reporter Molecules; Microbiology; Molecular Biology; Multiplex Cytometry for Soluble Analytes; Reproductive Technology; 12 Flowing On: The Future; General References; Glossary; A; B; C; D; E; F; G; H; I; L; M; N; O; P; Q; R; S; T; V; W; Figure Credits; Index; A; B; C; D; E; F; G; H; I; J; K; L; M ; NO; P; Q; R; S; T; U; V; W; X; Y; Z
• Sommario/riassunto: Flow cytometry continually amazes scientists with its ever-expanding utility. Advances in flow cytometry have opened new directions in theoretical science, clinical diagnosis, and medical practice. The new edition of Flow Cytometry: First Principles provides a thorough update of this now classic text, reflecting innovations in the field while outlining the fundamental elements of instrumentation, sample preparation, and data analysis. Flow Cytometry: First Principles, Second Edition explains the basic principles of flow cytometry, surveying its primary scientific and clinical a
• Titolo autorizzato: Flow cytometry
• ISBN: 1-118-68839-2; 1-280-54171-7; 9786610541713; 0-471-45911-9; 0-471-22394-8
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Lingua di pubblicazione: Inglese
• Record Nr.: 9910678158303321
• Lo trovi qui: Univ. Federico II