DNA vaccines : types, advantages and limitations / / Erin C. Donnelly and Arthur M. Dixon, editors

• Pubblicazione: Hauppauge, N.Y., : Nova Science, c2011
• Edizione: 1st ed.
• Descrizione fisica: 1 online resource (214 p.)
• Disciplina: 615/.372
• Soggetto topico: DNA vaccines
• Altri autori: DonnellyErin C DixonArthur M
• Note generali: Description based upon print version of record.
• Nota di bibliografia: Includes bibliographical references and index.
• Nota di contenuto: Intro -- DNA VACCINES: TYPES, ADVANTAGES AND LIMITATIONS -- DNA VACCINES: TYPES, ADVANTAGES AND LIMITATIONS -- Library of Congress Cataloging-in-Publication Data -- Contents -- Preface -- Chapter I Novel Carrier Adjuvant for DNA Vaccination -- Abstract -- 1. Introduction -- 2. Immunology of DNA Vaccines: Mode of Action and Immune Response Induction -- 3. Barriers in DNA Vaccine Delivery and Possible Modes to Overcome these Barriers -- 4. Novel Strategies for DNA Delivery -- 4.1. Lipid Based Carrier Adjuvants for DNA Vaccination -- 4.1.1. Liposomes -- 4.1.2. Virosomes -- 4.2. Polymers and Polymeric Carriers Systems -- 4.2.1. Polyethylenimine -- 4.2.2. Polylysine -- 4.2.3. Chitosan -- 4.2.4. Dendrimers -- 4.2.5. Poly (Lactide-Co-Glycolide) (PLG) -- 5. Concluding Remark and Future Prospects -- References -- Chapter II Improving the Immunogenicity of DNA Vaccines: A Nano-Sized Task? -- Abstract -- Introduction -- Improving Immunogenicity of Plasmid DNA Vaccines -- Routes of Delivery of Plasmid DNA Vaccines -- Physical Delivery Systems of Plasmid DNA Vaccines -- Needle-Free Delivery Methods -- Expression Optimization -- Cytokines -- Delivery Vectors for DNA Vaccines -- Bacterial Vectors -- Viral Vectors -- DNA and RNA Replicons -- Nano-Sized Vehicles for Gene Delivery -- Inorganic to Biomaterial - Past to Future -- Size Determines the Fate of Particles -- Conclusion -- References -- Chapter III Novel Delivery Strategies for DNA -- Abstract -- I. Introduction -- II. Optimization of Immunogenicity of DNA Vaccines -- 2.1. Choice of Targets and Expression Plasmid for DNA Vaccines -- 2.2. Selection of High Expression Plasmids for DNA Vaccines -- 2.3. Codon Optimization for Higher Gene Expression -- 2.4. Adjuvant Choice for DNA Vaccines -- III. DNA Delivery Strategies -- 3.1. Invasive Delivery -- 3.1.1. Needle Injection. ; 3.1.2. In Vivo Electroporation -- 3.1.3. Tattoo Gun Delivery -- 3.2. Non-Invasive Topical Delivery Method -- 3.2.1. Tape Stripping -- 3.2.2. Nanopatch Delivery -- 3.2.3. Nano-Particle Mediated Delivery -- 3.3. Viral-Mediated DNA Delivery Systems -- 3.4. Non-Viral Gene Delivery Systems -- 3.5. Novel Delivery Methods -- 3.5.1. Laser Mediated Delivery -- 3.5.2. Ultrasound Mediated Delivery System -- III. Future Directions -- References -- Chapter IV Recent Advances in DNA Vaccines -- Abstract -- Introduction -- DNA Vaccines X Traditional Vaccine Approaches -- Components of a DNA Vaccine -- How DNA Vaccines Work -- Delivery Methods -- Advantages X Limitations -- Strategies to Enhance Immunogenicity of DNA Vaccines -- Modifications of Plasmid Basic Design -- Delivery Methods -- Target to Dendritic Cells -- Improved Immunization Protocols -- Adjuvants -- Conclusion -- References -- Chapter V Co-Administration of Gamma Interferon Gene with DNA Vaccine Expressing Duck Hepatitis B Virus (DHBV) Proteins Enhances Therapeutic Efficacy of DNA-Based Immunization in Chronic Virus Carriers -- Abstract -- Introduction -- Materials and Methods -- Animals -- DNA Immunization -- Analysis of Viral DNA -- Analysis of Infectivity of Liver Extracts Collected from Resolved Ducks -- Histopathological and Immuno-Histochemical Analysis -- Statistical Analysis -- Results -- Decrease in Viremia following Co-Immunization with Cytokine Expressing Plasmids -- Enhancement of DHBV DNA clearance by Co-Immunization with IFN- expressing Plasmid -- Virological Analysis and Infectivity of Liver Samples Collected from Resolved Animals -- Adverse Effects of Therapy -- Conclusion -- Acknowledgments -- References -- Chapter VI The Role of DNA-IL-12 Vaccination in Eosinophilic Inflammation: A Review -- Abstract -- Basic DNA Vaccine Concepts -- Strategies for DNA Vaccine Administration. ; Structure and Function of Interleukin-12 -- IL-12 Therapy -- Eosinophilia and Asthma -- Impact of pcDNA-IL-12 Administration on Eosinophilia and Pulmonary Hyperreactivity in a Murine Model -- References -- Chapter VII Cured By DNA-Genetic Immunization in the Therapeutic Sector -- Abstract -- Introduction -- Modulation of the Immune System -- The Big Four - Cancer, HIV, Hepatitis and Tuberculosis -- Cancer -- HIV -- Hepatitis -- Tuberculosis -- Clinical Trials -- Safety Issues, Limitations and Disadvantages -- Conclusion -- References -- Chapter VIII DNA Vaccination against Herpesvirus -- Abstract -- Introduction -- Bovine Herpesvirus-1 -- DNA Vaccine against Bovine Herpesvirus-1 -- Route of Immunization -- Chemical Adjuvants -- Animal Response Following -- DNA Vaccination -- References -- Chapter IX DNA Vaccination: Progress and Challenges -- Abstract -- Abbreviations -- Introduction -- Advantages of DNA Vaccines -- Limitations of DNA Vaccines -- Conclusion -- Acknowledgments -- References -- Index.
• Sommario/riassunto: DNA vaccination is one of the most promising techniques for immunisation against diseases caused by viruses, protozoa, bacteria, and even for tumours and illnesses with genetic origins. These vaccines can be administered by direct inoculation of plasmid by several routes. In this book, the authors present topical research in the study of the types, advantages and limitations of DNA vaccines. Topics discussed include the role of novel carrier constructs in effective and safe DNA vaccine delivery; improving the immunogenicity of DNA vaccines; the role of DNA-IL-12 vaccination in cosinphilic inflammation; genetic immunisation enabling re-direction of the host immune system resulting in the development of effective immune responses; and DNA vaccination against herpesvirus.
• Titolo autorizzato: DNA vaccines
• ISBN: 1-62081-045-X
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Lingua di pubblicazione: Inglese
• Record Nr.: 9910958471103321
• Lo trovi qui: Univ. Federico II
• Serie: Immunology and immune system disorders.