Decellularized materials : preparations and biomedical applications / / Xiaoming Li, Huiqi Xie, editors

• Pubblicazione: Gateway East, Singapore : , : Springer, , [2021]; ©2021
• Descrizione fisica: 1 online resource (517 pages)
• Disciplina: 610.28
• Soggetto topico: Tissue engineering; Enginyeria de teixits
• Soggetto genere / forma: Llibres electrònics
• Persona (resp. second.): LiXiaoming; XieHuiqi
• Nota di contenuto: Intro -- Acknowledgements -- Contents -- About the Editors -- Chapter 1: Overview of Decellularized Materials for Tissue Repair and Organ Replacement -- 1.1 Background of Decellularized Materials -- 1.2 Preparation of Decellularized Materials -- 1.3 Composition and Structure of Decellularized Materials -- 1.3.1 Composition of Decellularized Materials -- 1.3.1.1 Collagen -- 1.3.1.2 Elastin -- 1.3.1.3 Proteoglycan and Glycosaminoglycan -- 1.3.1.4 Fibronectin and Laminin -- 1.3.1.5 Growth Factor -- 1.3.1.6 Matrix-Bound Nanovesicles -- 1.3.2 Structure of Decellularized Materials -- 1.4 Degradation of Decellularized Materials -- 1.5 Mechanisms of Decellularized Materials on Promoting Tissue Repair/Organ Replacement -- 1.5.1 Initiating Relatively Low Host Tissue Response to Provide Regeneration Microenvironment -- 1.5.2 Containing Bioactive Factors to Recruit Endogenous Stems/Progenitor Cells and Promote Matrix Production and Angiogenesis -- 1.6 Decellularized Tissues and Their Applications -- 1.7 Decellularized Organs and Their Recellularization -- 1.8 The Structure and Main Content of this Book -- References -- Chapter 2: The Decellularization of Tissues -- 2.1 Description of Decellularization Protocols -- 2.1.1 Physical Methods -- 2.1.1.1 Freeze-Thaw -- 2.1.1.2 Ultrasound -- 2.1.1.3 Pressure Gradient -- 2.1.1.4 Supercritical Fluid Extraction -- 2.1.1.5 Electroporation -- 2.1.1.6 Immersion and Agitation -- 2.1.2 Chemical Methods -- 2.1.2.1 Alkaline and Acid Treatments -- 2.1.2.2 Non-ionic Detergents -- 2.1.2.3 Ionic Detergents -- 2.1.2.4 Zwitterionic Detergents -- 2.1.2.5 Tri(n-butyl)phosphate -- 2.1.2.6 Hypotonic and Hypertonic Treatments -- 2.1.2.7 Polyethylene Glycol -- 2.1.2.8 Alcohols and Acetone -- 2.1.3 Biological Methods -- 2.1.3.1 Nuclease -- 2.1.3.2 Trypsin -- 2.1.3.3 Dispase -- 2.1.3.4 Lipase -- 2.1.3.5 α-Galactosidase. ; 2.1.3.6 Chelating Agents -- 2.1.4 New Methods -- 2.1.4.1 Apoptosis -- 2.1.4.2 Serum -- 2.1.5 Combinatorial Methods -- 2.2 Evaluation of Decellularization -- 2.2.1 Establishing Metrics for Effective Decellularization -- 2.2.2 Evaluation Methods -- 2.2.2.1 Histological Analysis -- 2.2.2.2 Immunohistochemistry -- 2.2.2.3 DNA Assay -- 2.2.2.4 SDS-PAGE -- 2.2.2.5 Mechanical Testing -- 2.2.3 Effect of Ineffective Decellularization -- 2.3 Subsequent Cleaning and Sterilization -- 2.3.1 Subsequent Cleaning of Decellularized Tissues -- 2.3.2 Regulatory Requirements for Sterilization -- 2.3.3 Sterilization of Decellularized Tissues -- 2.3.3.1 γ-Irradiation -- 2.3.3.2 Ethylene Oxide Exposure -- 2.3.3.3 Supercritical Carbon Dioxide -- 2.4 Conclusions and Future Considerations -- References -- Chapter 3: Different Forms of Decellularized Tissues and Their Characteristics, Applications in Tissue Repair as Well as Perfo... -- 3.1 Scaffolds in Sheet and Tubular -- 3.2 Extracellular Matrix Powders -- 3.2.1 Preparation Methods and Characteristics -- 3.2.2 Optimization and Applications -- 3.2.3 Usage Concentration -- 3.2.4 Particle Morphology -- 3.2.5 Sterilization Methods -- 3.2.6 Solubilization Properties -- 3.2.7 Crosslinking Methods -- 3.2.8 The Applications of ECM Powder in Tissue Engineering -- 3.2.8.1 ECM Powder as Carrier -- 3.2.8.2 Cell Culture Carrier -- 3.2.8.3 Drug Delivery Vehicle -- 3.2.8.4 Powder Substrate -- 3.2.8.5 ECM Modification -- 3.2.8.6 3D Bioprinting Ink -- 3.2.8.7 Clinical Application of Powder-Based ECM -- 3.3 Extracellular Matrix Hydrogels -- 3.3.1 Preparation Methods -- 3.3.2 Characteristics -- 3.3.2.1 Microstructure -- 3.3.2.2 Rheological Properties -- 3.3.3 Applications and Efforts in Performance Optimization -- 3.4 Extracellular Matrix Coating -- 3.4.1 Preparation Methods and Characteristics. ; 3.4.2 Applications and Efforts in Performance Optimization -- 3.5 3D Printing Tissue ECM Scaffolds -- 3.5.1 Preparation Methods and Characteristics -- 3.5.2 Applications and Efforts in Performance Optimization -- 3.6 Conclusions -- References -- Chapter 4: Applications of Decellularized Materials for Tissue Repair -- 4.1 Introduction -- 4.2 Decellularized Materials for Musculoskeletal Tissue Engineering -- 4.2.1 Bone Tissue Engineering -- 4.2.1.1 Structure and Characteristics of Bone -- 4.2.1.2 Requirements for Bone Tissue Engineering Materials -- 4.2.2 Cartilage Tissue Engineering -- 4.2.2.1 Structure and Characteristics of Cartilage -- 4.2.2.2 Requirements for Cartilage Tissue Engineering Materials -- 4.2.2.3 Applications of Decellularized Materials in Cartilage -- 4.2.3 Ligament and Tendon Tissue Engineering -- 4.2.3.1 Structure and Characteristics of Tendon and Ligament -- 4.2.3.2 Requirements for Tendon and Ligament Tissue Engineering Materials -- 4.2.3.3 Applications of Decellularized Materials in Tendon and Ligament -- 4.3 Decellularized Materials for Skin Tissue Engineering -- 4.3.1 Structure and Characteristics of Skin -- 4.3.2 Requirements for Skin Tissue Engineering Materials -- 4.3.3 Applications of Decellularized Materials in Skin -- 4.4 Decellularized Materials for Bladder Tissue Engineering -- 4.4.1 Structure and Characteristics of Bladder -- 4.4.2 Requirements for Bladder Tissue Engineering Materials -- 4.4.3 Applications of Decellularized Materials in Bladder -- 4.5 Decellularized Materials for Abdominal Wall Tissue Engineering -- 4.5.1 Structure and Characteristics of Abdominal Wall -- 4.5.2 Requirements for Abdominal Wall Tissue Engineering Materials -- 4.5.3 Applications of Decellularized Materials in Abdominal Wall -- 4.6 Decellularized Materials for Cardiovascular Tissue Engineering. ; 4.6.1 Structure and Characteristics of Cardiovascular -- 4.6.2 Requirements for Cardiovascular Tissue Engineering Materials -- 4.6.3 Applications of Decellularized Materials in Cardiovascular -- 4.7 Others -- 4.7.1 Trachea Tissue Engineering -- 4.7.1.1 Structure and Characteristics of Trachea Tissue -- 4.7.1.2 Requirements of Trachea Tissue Engineering -- 4.7.1.3 Applications of Decellularized Materials for Trachea Tissue Engineering -- 4.7.2 Gastrointestinal Tissue Engineering -- 4.7.2.1 Structure and Characteristics of Gastrointestinal -- 4.7.2.2 Requirements for Gastrointestinal Tissue Engineering Materials -- 4.7.2.3 Applications of Decellularized Materials in Gastrointestinal -- 4.7.3 Esophagus Tissue Engineering -- 4.7.3.1 Structure and Characteristics of Esophagus -- 4.7.3.2 Requirements for Esophagus Tissue Engineering Materials -- 4.7.3.3 Applications of Decellularized Materials in Esophagus -- 4.7.4 Nerve Tissue Engineering -- 4.7.4.1 Structure and Characteristics of Nerve -- 4.7.4.2 Requirements for Nerve Tissue Engineering Materials -- 4.7.4.3 Applications of Decellularized Materials in Nerve -- References -- Chapter 5: The Decellularization of Whole Organs -- 5.1 Decellularization Method -- 5.1.1 Physical Method -- 5.1.2 Chemical Method -- 5.1.3 Enzyme Decellularization -- 5.1.4 Decellularized Perfusion -- 5.2 General Effective Standards for the Decellularization of Organs -- 5.2.1 Cell Composition -- 5.2.2 ECM Structure -- 5.2.3 ECM Composition -- 5.2.4 Immunological Analysis -- 5.2.5 Mechanical Strength -- 5.2.6 Detergent Residue -- 5.3 The Decellularization of Different Whole Organs -- 5.3.1 Heart -- 5.3.2 Lung -- 5.3.3 Liver -- 5.3.4 Kidney -- 5.3.5 Pancreas -- 5.3.6 Intestine -- 5.3.7 Uterus -- References -- Chapter 6: Recellularization of Decellularized Whole Organ Scaffolds: Elements, Progresses, and Challenges -- 6.1 Introduction. ; 6.2 Cell Sources for Whole Organ Engineering -- 6.2.1 General Considerations -- 6.2.1.1 Differentiated Versus Stem Cells -- 6.2.1.2 Autologous Versus Allogenic Cells -- 6.2.1.3 Other Considerations -- 6.2.2 Cell Lines -- 6.2.3 Endothelial Progenitor Cells and Endothelial Cells -- 6.2.3.1 Endothelial Progenitor Cells (EPCs) -- 6.2.3.2 Endothelial Cells (ECs) -- 6.2.4 Organ-Derived Cells for Whole Organ Engineering -- 6.2.4.1 Differentiated Cells Isolated from the Organ of Interest -- 6.2.4.2 Organ-Derived Stem/Progenitor Cells -- 6.2.5 Stem Cells for Whole Organ Engineering -- 6.2.5.1 ESCs for Whole Organ Engineering -- 6.2.5.2 iPSCs for Whole Organ Engineering -- 6.2.5.3 Adult Stem Cells for Whole Organ Engineering -- Perinatal Stem Cells -- Mesenchymal stem cells (MSCs) -- 6.3 Decellularization of Whole Organ Scaffolds -- 6.3.1 Decellularization Methods -- 6.3.1.1 Set up a Perfusion System -- 6.3.1.2 Factors Used in Whole Organ Decellularization -- Physical Factors -- Chemical Factors -- Enzymatic Factors -- 6.3.1.3 Sterilization of the Whole Organ Scaffolds -- 6.3.2 Evaluation of Decellularization -- 6.3.2.1 Integrity of Vascular Structures -- 6.3.2.2 Detergent Residual -- 6.3.2.3 Cellular Components -- 6.3.2.4 ECM Components -- 6.3.2.5 Mechanics of the Decellularized Scaffolds -- 6.3.3 Decellularized Whole Organ Scaffolds -- 6.3.3.1 Decellularized Heart Scaffolds -- 6.3.3.2 Decellularized Liver Scaffolds -- 6.3.3.3 Decellularized Lung Scaffolds -- 6.3.3.4 Decellularized Kidney Scaffolds -- 6.3.3.5 Decellularized Pancreas Scaffolds -- 6.4 Recellularization Methods for Whole Organ Engineering -- 6.4.1 Recellularization Methods -- 6.4.1.1 Cell Delivery -- Cell Number -- Delivery Routes -- Cell Concentration -- 6.4.1.2 Perfusion Culture -- 6.4.1.3 Endpoints -- 6.4.2 Bioreactor -- 6.4.2.1 The Basic Framework of a Bioreactor. ; 6.4.2.2 Organ-Specific Bioreactors.
• Titolo autorizzato: Decellularized materials
• ISBN: 981-336-962-0
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Lingua di pubblicazione: Inglese
• Record Nr.: 9910484442703321
• Lo trovi qui: Univ. Federico II