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[null,"1.","Record Nr.",null,"UNINA990002760290403321"]
[null,null,"Autore",null,"Johansen, Leif <1930-1982>"]
[null,null,"Titolo",null,"Production functions. / by Leif Johansen"]
[null,null,"Pubbl/distr/stampa",null,"Amsterdam : North Holland, 1972"]
[null,null,"Locazione",null,"ECA"]
[null,null,"Collocazione",null,"7-0-16"]
[null,null,"Lingua di pubblicazione",null,"Inglese"]
[null,null,"Formato",null,"Materiale a stampa"]
[null,null,null,"Livello bibliografico","Monografia"]
[null,"2.","Record Nr.",null,"UNINA9910966245903321"]
[null,null,"Titolo",null,"Caco-2 cells and their uses / / Megan A. Schulz, editor"]
[null,null,"Pubbl/distr/stampa",null,"Hauppauge, N.Y., : Nova Science Publishers, Inc., c2011"]
[null,null,"ISBN",null,"1-62081-613-X"]
[null,null,"Edizione",null,"[1st ed.]"]
[null,null,"Descrizione fisica",null,"1 online resource (219 p.)"]
[null,null,"Collana",null,"Cell biology research progress"]
[null,null,"Disciplina",null,"611/.0187"]
[null,null,"Soggetti",null,"Epithelial cells"]
[{"$nulls":4},"Monomolecular films"]
[{"$nulls":4},"Intestinal absorption"]
[null,null,"Lingua di pubblicazione",null,"Inglese"]
[null,null,"Formato",null,"Materiale a stampa"]
[null,null,null,"Livello bibliografico","Monografia"]
[null,null,null,"Note generali","Description based upon print version of record."]
[null,null,null,"Nota di bibliografia","Includes bibliographical references and index."]
[null,null,"Nota di contenuto",null,"Intro -- CACO-2 CELLS AND THEIR USES -- CACO-2 CELLS AND THEIR USES -- Library of Congress Cataloging-in-Publication Data -- CONTENTS -- PREFACE -- Chapter 1  THE INDUSTRIAL AND ACADEMIC RATIONALE OF CACO-2 CELL MODELS TODAY -- ABSTRACT -- ABBREVIATIONS -- 1. INTRODUCTION -- 2. CACO-2 CELL ADVANTAGES, DRAWBACKS  AND TRANSPORTER-ENZYME EXPRESSION PATTERNS -- 2.1. Applicability of CACO-2 Models to Predict the Fraction  of Drug Absorbed in Humans -- 2.2. CACO-2 Transporters and Enzyme Expression and the Comparison with the In Vivo Human Expression -- 3. CACO-2 CELLS AS AN IN VITRO PROGNOSTIC TOOL FOR ASSESSING PHARMACOKINETIC DRUG-GARLIC INTERACTIONS -- 4. CACO-2 CELLS FOR THE EVALUATION OF SELF-MICROEMULSIFYING DRUG DELIVERY SYSTEMS (SMEDDS) -- 4.1. Self-Microemulsifying Drug Delivery Systems:The Objectives and Advantages -- 4.2. Studies Performed to Evaluate SMEDDS Impact on Intestinal Absorption of Drugs -- 4.3. The Mechanism Responsible for Increased Absorption of Furosemide Incorporated in SMEDDS -- CONCLUSION -- REFERENCES -- Chapter 2  A CRITICAL PERSPECTIVE ON CELL LINES STUDIES IN NUTRITION: THE CASE OF INTESTINAL ABSORPTION -- ABSTRACT -- INTRODUCTION -- RESEARCH WITH CELL LINES -- CELL LINES IN INTESTINAL ABSORPTION STUDIES -- NON-HUMAN CELL LINES IN INTESTINAL ABSORPTION STUDIES -- IN VITRO CELL CULTURES AS AN ALTERNATIVE  TO ANIMAL TESTING -- INTESTINAL CELL LINES AS AN IN VITRO MODEL  OF CLINICAL VALUE -- CONCLUSION -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 3  CO-CULTURE "]
[{"$nulls":4},"SYSTEMS WITH CACO-2 CELLS TO EVALUATE THE BIOACTIVITY OF PLANT EXTRACTS -- ABSTRACT -- INTRODUCTION -- CO-CULTURE SYSTEMS USING CACO-2 CELLS:  CELL VIABILITY AND FUNCTIONALITY -- WORKING WITH NATURAL EXTRACTS: CACO-2 AS A FILTER TO SIMULATE THE HUMAN INTESTINAL BARRIER -- WORKING WITH NATURAL EXTRACTS: ARE THESE FILTERED EXTRACTS BIOACTIVE? -- CONCLUSION."]
[{"$nulls":4},"ACKNOWLEDGMENTS -- REFERENCES -- Chapter 4  THE USE OF CACO-2 CELLS IN THE STUDY OF INTESTINAL TRANSPORT -- ABSTRACT -- INTRODUCTION -- USES OF CACO-2 CELLS -- Paracellular Pathway -- Transcellular Pathway -- CONCLUSION -- REFERENCES -- Chapter 5  CACO-2 CELLS AS AN EXPERIMENTAL MODEL FOR THE STUDY OF CELL-MATRIX INTERACTIONS AND WOUND HEALING IN INTESTINAL VILLUS CELLS -- ABSTRACT -- INTRODUCTION -- CACO-2 CELLS AND CELL-MATRIX INTERACTIONS -- CACO-2 CELLS AND CELL-MATRIX INTERACTIONS IN CO-CULTURE -- CACO-2 CELLS IN CO-CULTURE: A WOUND HEALING MODEL -- CONCLUSIONS -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 6  EFFECT OF POLYUNSATURATED FATTY ACIDS AND BILE SALTS ON BUTYRATE UPTAKE BY INTESTINAL EPITHELIAL CELLS -- ABSTRACT -- INTRODUCTION -- MATERIALS AND METHODS -- CACO-2 Cell Culture -- IEC-6 Cell Culture -- Determination of 14C-BT uptake by CACO-2 and IEC-6 Cells -- Treatment of the Cells with Compounds -- Determination of Cell Viability -- Protein Determination -- Calculation and Statistics -- Materials -- RESULTS -- Effect of Pufas and Bile Acids on 14C-BT Apical uptake by CACO-2 Cells -- Effect of Pufas and Bile Acids on 14C-BT Apical uptake by IEC-6 Cells -- CONCLUSION -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 7  ANALYSIS OF BOTULINUM TOXIN: INTESTINAL EPITHELIAL BARRIER INTERACTION USING EPITHELIAL MONOLAYER CULTURE SYSTEMS -- ABSTRACT -- INTRODUCTION -- 1. TRANSCYTOSIS OF BONT ACROSS CULTURED INTESTINAL EPITHELIAL MONOLAYERS -- 2. ADHESION OF HA TO INTESTINAL EPITHELIAL CELLS -- 3. DISRUPTION OF THE INTERCELLULAR EPITHELIAL BARRIER BY HA -- 4. POSSIBLE MECHANISMS OF BONT COMPLEX TRANSPORT ACROSS THE INTESTINAL EPITHELIAL MONOLAYER -- CONCLUSION -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 8  CACO-2 CELLS AND GENOTOXICITY STUDIES OF FOOD CONTAMINANTS -- ABSTRACT -- INTRODUCTION ON IN VITRO GENOTOXICITY TESTING."]
[{"$nulls":4},"IN VITRO HUMAN INTESTINAL CELLS: PERTINENCE FOR GENOTOXICITY TESTING OF FOOD CONTAMINANTS -- USE OF CACO-2 CELLS IN GENOTOXICITY TESTS -- Comet Assay -- Others -- CYTOKINESIS-BLOCK MICRONUCLEUS ASSAY -- On Undifferentiated Cells -- On Differentiated Cells: Development and Application -- IMPROVEMENT OF THIS CELL MODEL TO PREDICT TOXICITY AND MECHANISTIC ENDPOINTS -- Co-Cultures CACO-2/TK6 -- Metabolism Regulation -- CONCLUSIONS -- ACKNOWLEDGMENT -- REFERENCES -- Chapter 9  CACO-2 CELLS FOR STUDYING INTESTINAL ABSORPTION MECHANISMS: COMPARISON AND CORRELATIONS WITH RAT MODEL -- ABSTRACT -- INTRODUCTION: IN VITRO MODELS OF INTESTINAL PERMEATION -- ABSORPTION IN THE GASTROINTESTINAL TRACT: ROUTES OF PERMEATION ACROSS INTESTINAL CELLS. -- IN VITRO MODELS OF THE INTESTINAL BARRIER:  CELL MODELS -- CACO-2 -- Other Cell Lines -- HT29 Cells -- CACO-2 CELLS FOR PREDICTING ORAL FRACTION ABSORBED: COMPARISON WITH HUMAN, RAT AND PAMPA MODELS -- CACO-2 CELLS FOR THE STUDY OF DRUG ABSORPTION MECHANISMS: MODELING APPROACHES FOR PARAMETER ESTIMATION -- Apparent Permeability Estimation (Sink versus Non Sink Conditions) -- Mathematical Modeling of CACO-2 Data for Parameter Estimation -- P "]
[{"$nulls":4},"versus C -- Amounts versus Time -- CORRELATION BETWEEN CACO-2 CELLS DATA AND IN SITU RAT DATA FOR THE STUDY OF P-GP MEDIATED SECRETION -- CONCLUSIONS -- REFERENCES -- Chapter 10  NANOCARRIER ABSORPTION STUDIES  WITH CACO-2 CELLS -- ABSTRACT -- INTRODUCTION -- I. METHOD -- a. Absorption Study -- b. Mechanistic Studies -- II. RESULTS -- a. Absorption Study -- b. Mechanistics Study -- III. DISADVANTAGE/ LIMIT OF CACO-2 TO STUDY DRUG TRANSPORT / OTHER METHOD -- a. Parallel Artificial Membrane Permeation Assay (PAMPA) -- b. Alternative Cell Culture Models -- c. In Vivo or Ex Vivo Studies -- CONCLUSION -- ACKNOWLEDGMENT -- BIBLIOGRAPHY."]
[{"$nulls":4},"Chapter 11  CACO-2 CELLS AS A MODEL TO STUDY THE INTESTINAL EFFECTS OF NON-ABSORBABLE PHYTOCHEMICALS -- ABSTRACT -- ABBREVIATIONS -- 1. INTRODUCTION -- 2. PROCYANIDINS:STRUCTURE AND METABOLISM -- 3. PROCYANIDINS AND THE HEALTH  OF THE GASTROINTESTINAL TRACT -- 4. INTERACTIONS OF PROCYANIDINS WITH CACO-2 CELL MEMBRANES -- 5. PROCYANIDINS PROTECTCACO-2 CELLSFROM OXIDATION AND PERMEABILIZATION -- 6. PROCYANIDINS PROTECTCACO-2  CELLS FROM INFLAMMATION -- CONCLUSION -- ACKNOWLEDGMENTS -- REFERENCES -- INDEX."]
[null,null,null,"Sommario/riassunto","Presents topical research in the study of CACO-2 cells and their uses. This book discusses topics that include the application of CACO-2 cell models as a supportive in vitro tool during drug discovery and formulation; co-culture systems with CACO-2 cells to evaluate the bioactivity of plant extracts; and, more."]