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[null,"1.","Record Nr.",null,"UNINA9910955394303321"]
[null,null,"Titolo",null,"Mutation detection : a practical approach / / edited by R.G.H. Cotton, E. Edkins and S. Forrest"]
[null,null,"Pubbl/distr/stampa",null,"Oxford : , : IRL Press at Oxford University Press, , 2023"]
[null,null,"ISBN",null,"1-383-04935-1"]
[{"$nulls":4},"0-19-156569-5"]
[{"$nulls":4},"1-280-37548-5"]
[{"$nulls":4},"9786610375486"]
[{"$nulls":4},"0-585-48413-9"]
[null,null,"Edizione",null,"[1st ed.]"]
[null,null,"Descrizione fisica",null,"1 online resource (263 p.)"]
[null,null,"Collana",null,"Practical approach series"]
[{"$nulls":4},"Oxford scholarship online"]
[{"$nulls":4},"Practical approach series ; ; 188"]
[null,null,"Disciplina",null,"576.5/49"]
[null,null,"Soggetti",null,"Mutation (Biology)"]
[{"$nulls":4},"Molecular genetics"]
[{"$nulls":4},"Chromosome abnormalities"]
[null,null,"Lingua di pubblicazione",null,"Inglese"]
[null,null,"Formato",null,"Materiale a stampa"]
[null,null,null,"Livello bibliografico","Monografia"]
[null,null,null,"Note generali","Previously issued in print: 1998."]
[null,null,null,"Nota di bibliografia","Includes bibliographical references."]
[null,null,"Nota di contenuto",null,"Cover; Contents; List of Contributors; Abbreviations; Introduction; References; 1. Single-strand conformation polymorphism analysis; 1. Introduction; 2. PCR-SSCP using polyacrylamide slab gel; PCR Optimization and primer design; Pre-amplification and isolation by agarose gel electrophoresis; PCR using [[Sup(32)]P]deoxynucleotide triphosphate; Removal of 3' appendage; SSCP gel electrophoresis; Interpretation of autoradiogram; Re-amplification and direct sequencing; Gel matrices other than polyacrylamide; Restriction endonuclease fingerprinting and dideoxy fingerprinting"]
[{"$nulls":4},"3. Fluorescent SSCP in an automated DNA sequencerPrimer design in post-PCR fluorescent labelling; Fluorescent labelling by 3' exchange reaction; SSCP in capillary electrophoresis (CE-SSCP); Data processing; Acknowledgements; References; 2. Single-stranded conformation polymorphism and heteroduplex analysis; 1. Introduction; 2. Optimization of the PCR reaction; 3. SSCP sample prepration; 4. Optimization of SSCP/HA detection; 5. Multiplexing; 6. Interpretation of results; 7. Applications; 8. Other methods; References"]
[{"$nulls":4},"3. Comprehensive mutation detection with denaturing gradient gel electrophoresis1. Introduction; The scope of DGGE, its distinctive capabilities, and the nature of results; 2. Background; 3. Basic principle, the physical properties of DNA; 4. Overview of the procedures in searching for mutants; Defining segments for scrutiny; Sample preparation; Gradient and velocity separations; Features of the gel patterns; Discrimination of zygozygosity; Comments; 5. Use of the psoralen cross-link as a clamp; The psoralen protocol; 6. Computational tools; What is a meltmap?; Meltmap protocol"]
[{"$nulls":4},"Predicting electrophoretic separationsComputer operations for MUTRAV; 7. Other members of the DGGE family; Gel separations in a uniform, partially denaturing environment; Capillary electrophoresis; The thermal gradient; The temperature ramp; 2D length and gradient separations; 8. End notes; Acknowledgments; References; 4. Cleavage using RNase to detect mutations; 1. Introduction; 2. RNase protection assay for mutation detection; Evaluation of the sensitivity; Source material; PCR for RNase protection assay; RNA probe preparation; RNase protection; Detection of digested probe"]
[{"$nulls":4},"Mutation detection by sequencing of the PCR productsOther modified methodologies for mutation detection; Acknowledgements; References; 5. Cleavage of mismatched bases using chemical reagents; 1. Introduction; 2. Basic procedures; Comments on the basic procedures; 3. Ultra fast chemical mismatch detection; Labelling; Solid phase; Comments; References; 6. Mutation detection using T4 endonuclease VII; 1. Introduction; 2. The biology of Endo VII; The role of Endo VII in vivo; Characterization of Endo VII; Action of Endo VII on heteroduplex DNA; 3. Use of Endo VII for mutation detection"]
[{"$nulls":4},"Enzyme mismatch cleavage"]
[null,null,null,"Sommario/riassunto","This volume offers the latest tried and tested protocols for a range of detection methods, from the labs of the leading researchers in the field."]