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[null,"1.","Record Nr.",null,"UNINA9910954876203321"]
[null,null,"Autore",null,"Xia Wenshui"]
[null,null,"Titolo",null,"Chitosan hydrolysis by non-specific enzymes / / Wenshui Xia and Ping Liu"]
[null,null,"Pubbl/distr/stampa",null,"New York, : Nova Science Publishers, c2010"]
[null,null,"ISBN",null,"1-61324-220-4"]
[null,null,"Edizione",null,"[1st ed.]"]
[null,null,"Descrizione fisica",null,"1 online resource (78 p.)"]
[null,null,"Collana",null,"Environmental Science, Engineering and Technology"]
[null,null,"Disciplina",null,"612.3/9"]
[null,null,"Soggetti",null,"Chitosan - Metabolism"]
[null,null,"Lingua di pubblicazione",null,"Inglese"]
[null,null,"Formato",null,"Materiale a stampa"]
[null,null,null,"Livello bibliografico","Monografia"]
[null,null,null,"Note generali","Description based upon print version of record."]
[null,null,null,"Nota di bibliografia","Includes bibliographical references and index."]
[null,null,"Nota di contenuto",null,"Intro -- CHITOSAN HYDROLYSIS  BY NON-SPECIFIC ENZYMES -- CHITOSAN HYDROLYSIS  BY NON-SPECIFIC ENZYMES -- CONTENTS -- ABSTRACT -- Chapter 1  INTRODUCTION -- Chapter 2  CHARACTERIZATION OF CHITOSAN HYDROLYSIS BY NONSPECIFIC ENZYMES -- 2.1. CHARACTERIZATION OFCHITOSAN HYDROLYSIS  BY NON-SPECIFIC CELLULASES -- (1) Temperature and pH -- (2) Molecular Weight -- (3) Reduction of the Viscosity -- (4) Kinetic Parameters -- (5) Metal Ions -- 2.2. CHARACTERIZATION OF CHITOSAN HYDROLYSIS  BY NON-SPECIFIC LIPASE -- 2.3. Characterization of Chitosan Hydrolysis by Non-Specific Papain -- Chapter 3  MECHANISM OF NON-SPECIFIC ENZYMES TOWARD CHITOSAN -- 3.1. PURIFICATION AND CHARACTERIZATION  OF CHITOSANOLYTIC COMPONENTS FROM  NON -SPECIFIC ENZYMES -- 3.1.1. Purification and Characterization of a Bifunctional Enzyme with Chitosanolytic and Cellulolytic Activity from Commercial Cellulase -- 3.1.2. Purification and Characterization of a Bifunctional Chitosanase with Chitinase Activity from Commercial Lipase -- 3.2. ACTION MODE ANALYSIS OF BIFUNCTIONAL  ENZYMES ON CHITOSAN -- 3.2.1. Substrate Specificity -- 3.2.2. Chitosan and Chitooligosaccharide Hydrolysis Analysis by CCBE -- 3.2.3. TLC Analysis of COS Hydrolysis Products by the  Purified CNBE -- Chapter 4  STRUCTURE AND FUNCTION ANALYSIS  OF BIFUNCTIONAL ENZYMES WITH CHITOSANOLYTIC ACTIVITY -- 4.1. AMINO ACID COMPOSITION OF PURIFIED CCBE AND CNBE -- 4.2. STRUCTURE ANALYSIS OF PURIFIED CCBE AND CNBE -- 4.2.1. N-Terminal Sequencing Analysis of CNBE -- 4.2.2. Sequencing Analysis of the Purified CCBE by  MALDI-TOF Mass -- 4.3. DETERMINATION OF THE ACTIVE SITES OF CCBE  AND CNBE BY CHEMICAL MODIFICATION -- 4.3.1. The Essential Carboxyl Group "]
[{"$nulls":4},"Modification using EDC -- 4.3.2. Modification of Tryptophan Residues using NBS -- 4.3.3. Modification of Histidine Residue using DEPC."]
[{"$nulls":4},"4.3.4. Modification of Tyrosine, Threonine/Serine, Arginine, Methionine Residues Etc -- Chapter 5  IDENTIFICATION OF CCBE FROM T.VIRIDE  BY MOLECULAR CLONING -- 5.1. DETERMINATION OF FERMENTATION  CONDITION OF T.VIRIDE -- 5.2. CLONING AND SEQUENCE ANALYSIS OF CCBE GENE -- 5.3. EXPRESSION ANALYSIS OF CCBE SPLICING  GENES IN PICHIA PATORIS -- ACKNOWLEDGMENTS -- REFERENCE -- INDEX -- Blank Page."]
[null,null,null,"Sommario/riassunto","The focus of this book is the characterizations and hydrolyzing mechanism of the non-specific enzymes toward chitosan choosing the three typical non-specific enzymes: cellulase, lipase and papain as objects. The authors studied the enzymatic characteristics, purification, product analysis, glycoside bond cleavage, active sites and gene cloning of these enzymes to expatiate their non-specific hydrolysis mechanism."]