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[null,"1.","Record Nr.",null,"UNINA9910461071703321"]
[null,null,"Titolo",null,"Civilians and modern war : armed conflict and the ideology of violence / / edited by Daniel Rothbart, Karina V. Korostelina and Mohammed D. Cherkaoui"]
[null,null,"Pubbl/distr/stampa",null,"London : , : Routledge, , 2012"]
[null,null,"ISBN",null,"1-283-54634-5"]
[{"$nulls":4},"9786613858795"]
[{"$nulls":4},"1-136-33340-1"]
[{"$nulls":4},"0-203-12256-9"]
[null,null,"Descrizione fisica",null,"1 online resource (369 p.)"]
[null,null,"Collana",null,"War, conflict and ethics"]
[null,null,"Disciplina",null,"305.9/0695"]
[null,null,"Soggetti",null,"Civilians in war"]
[{"$nulls":4},"Combatants and noncombatants (International law)"]
[{"$nulls":4},"Civilian war casualties"]
[{"$nulls":4},"War victims"]
[{"$nulls":4},"Military history, Modern - 20th century"]
[{"$nulls":4},"Military history, Modern - 21st century"]
[{"$nulls":4},"War - Moral and ethical aspects"]
[{"$nulls":4},"Electronic books."]
[null,null,"Lingua di pubblicazione",null,"Inglese"]
[null,null,"Formato",null,"Materiale a stampa"]
[null,null,null,"Livello bibliografico","Monografia"]
[null,null,null,"Note generali","Description based upon print version of record."]
[null,null,null,"Nota di bibliografia","Includes bibliographical references and index."]
[null,null,"Nota di contenuto",null,"Cover; Civilians and Modern War; Copyright; Contents; List of figures and tables; Notes on contributors; Acknowledgments; 1. The place and plight of civilians in modern war; Part I: Targeting civilians; 2. The role of civilians in American war ideology; 3. Devastating civilians at home: the plight of Crimean Tatars and Californians of Asian descent during World War; 4. Military culture and civilian victimization: the Allied bombing of Germany in World War II; 5. Double victims: the recruitment and treatment of child soldiers in Chechnya; Part II: Preserving civilian immunity"]
[{"$nulls":4},"6. The politics of civilian identity7. Israeli soldiers' perceptions of Palestinian civilians during the 2009 Gaza War; 8. Civilian vulnerability in asymmetric conflict: lessons from the Second Lebanon and Gaza Wars; 9. In the shadow of soldiers: faceless victims in public media narrative; 10. Civilians, pundits, and the mediatized ideology; Part III: "]
[{"$nulls":4},"Redressing anti-civilian; 11. Trans-regional military dimensions of civilian protection: a two-part problem with a two-part solution; 12. Civilians under the law: inequality, universalisms, and intersectionality as intervention"]
[{"$nulls":4},"13. The price of justice14. Preventing genocide: towards systematic engagement by states; 15. Making amends: a new expectation for civilian losses in armed conflict; 16. Conclusion: the road ahead; Name index; Subject index"]
[null,null,null,"Sommario/riassunto","This book explores the issue of civilian devastation in modern warfare, focusing on the complex processes that effectively establish civilians' identity in times of war.Underpinning the physicality of war's tumult are structural forces that create landscapes of civilian vulnerability. Such forces operate in four sectors of modern warfare: nationalistic ideology, state-sponsored militaries, global media, and international institutions. Each sector promotes its own constructions of civilian identity in relation to militant combatants: constructions that prove lethal to the civil"]
[null,"2.","Record Nr.",null,"UNINA9910830656003321"]
[null,null,"Titolo",null,"Handbook of RNA biochemistry / / edited by Roland K. Hartmann"]
[null,null,"Pubbl/distr/stampa",null,"Weinheim : , : John Wiley & Sons, Inc., , 2014"]
[null,null,"ISBN",null,"3-527-64706-6"]
[null,null,"Edizione",null,"[Second edition.]"]
[null,null,"Descrizione fisica",null,"1 online resource (xlii, 1314 pages)"]
[null,null,"Disciplina",null,"574.192"]
[null,null,"Soggetti",null,"Biochemistry"]
[{"$nulls":4},"Biochemistry - Outlines, syllabi, etc"]
[null,null,"Lingua di pubblicazione",null,"Inglese"]
[null,null,"Formato",null,"Materiale a stampa"]
[null,null,null,"Livello bibliografico","Monografia"]
[null,null,"Nota di contenuto",null,"Cover; Title Page; Copyright; Contents; Preface; List of Contributors; Part I RNA Synthesis and Detection; Chapter 1 Enzymatic RNA Synthesis Using Bacteriophage T7 RNA Polymerase; 1.1 Introduction; 1.2 Description of Method -- T7 Transcription In vitro; 1.2.1 Templates; 1.2.1.1 Strategy (i): Insertion into a Plasmid; 1.2.1.2 Strategy (ii): Direct Use of Templates Generated by PCR; 1.2.1.3 Strategy (iii): Annealing of a T7 Promoter DNA Oligonucleotide to a Single-Stranded Template; 1.2.2 Special Demands on the RNA Product 1.2.2.1 Homogeneous 5' and 3' Ends, Small RNAs, Functional Groups at the 5' End1.2.2.2 Modified Substrates; 1.3 Transcription Protocols; 1.3.1 Transcription with Unmodified Nucleotides; 1.3.2 Transcription with 2' -Fluoro-Modified Nucleotides; 1.3.3 T7 Transcripts with 5' -- Cap Structures; 1.3.4 Purification; 1.4 Troubleshooting; 1.4.1 Low or No Product Yield; 1.5 Rapid Preparation of T7 RNA Polymerase; 1.5.1 Required Material; 1.5.1.1 Medium; 1.5.1.2 Buffers and Solutions; 1.5.1.3 Electrophoresis and Chromatography; 1.5.2 Procedure 1.5.2.1 Cell Growth, Induction, and Test for Expression of T7 RNAP1.5.2.2 Purification of T7 RNAP; 1.5.3 Notes and Troubleshooting; References; Chapter 2 Production of RNAs with Homogeneous 5' -- and 3' -Ends; 2.1 Introduction; 2.2 Description of Approach; 2.2.1 Cis-Cleaving Autocatalytic Ribozyme Cassettes; 2.2.1.1 The 5' -Cassette; 2.2.1.2 The 3' -Cassette; 2.2.1.3 Purification of Released RNA Product and Conversion of End Groups; 2.2.2 Trans-Cleaving Ribozymes for the Generation of Homogeneous 3' Ends; 2.2.3 Further Strategies toward Homogeneous Ends 2.3 Critical Experimental Steps, Changeable Parameters, Troubleshooting2.3.1 Construction of Cis-Cleaving 5'- and 3'-Cassettes; 2.4 PCR Protocols; "]
[{"$nulls":4},"2.5 Potential Problems; References; Chapter 3 RNA Ligation; 3.1 General Introduction; 3.1.1 T4 Polynucleotide Ligases; 3.1.2 Reaction Mechanism; 3.1.3 Advantages of T4 DNA Ligase for RNA Ligation; 3.1.4 Chapter Structure; 3.2 RNA Ligation Using T4 DNA Ligase (T4 Dnl); 3.2.1 Overview of the RNA Ligation Method Using the T4 DNA Ligase (T4 Dnl); 3.2.2 Large-Scale Transcription and Purification of RNAs 3.2.3 Generating Homogeneous Acceptor 3' -Ends for Ligation3.2.4 Site-Directed Cleavage with RNase H; 3.2.5 Dephosphorylation and Phosphorylation of RNAs; 3.2.6 RNA Ligation; 3.2.7 Troubleshooting; 3.3 Simultaneous Splint Ligation of Five RNA Fragments to Generate RNAs for FRET Experiments; 3.3.1 Introduction; 3.3.2 Construct Design; 3.3.3 Troubleshooting; 3.3.3.1 Low Overall Ligation Efficiency; 3.3.3.2 Undesired Ligation By-products; 3.3.3.3 RNA Degradation; 3.4 T4 RNA Ligase(s); 3.4.1 Introduction; 3.4.2 Mechanism and Substrate Specificity; 3.4.2.1 Early Studies."]
[null,null,null,"Sommario/riassunto","The second edition of a highly acclaimed handbook and ready reference. Unmatched in its breadth and quality, around 100 specialists from all over the world share their up-to-date expertise and experiences, including hundreds of protocols, complete with explanations, and hitherto unpublished troubleshooting hints. They cover all modern techniques for the handling, analysis and modification of RNAs and their complexes with proteins. Throughout, they bear the practising bench scientist in mind, providing quick and reliable access to a plethora of solutions for practical questions of RNA research."]