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[null,"1.","Record Nr.",null,"UNINA9910144117403321"]
[null,null,"Titolo",null,"Proteomics in practice : a guide to successful experimental design / / edited by Reiner Westermeier, Tom Naven, Hans-Rudolf Höpker"]
[null,null,"Pubbl/distr/stampa",null,"Weinheim, Germany : , : WILEY-VCH Verlag GmbH & Co. KGaA, , 2008"]
[{"$nulls":4},"©2008"]
[null,null,"ISBN",null,"1-281-94680-X"]
[{"$nulls":4},"9786611946807"]
[{"$nulls":4},"3-527-62229-2"]
[{"$nulls":4},"3-527-62230-6"]
[null,null,"Edizione",null,"[Second, completely revised edition.]"]
[null,null,"Descrizione fisica",null,"1 online resource (504 p.)"]
[null,null,"Disciplina",null,"572.6"]
[{"$nulls":4},"572.64"]
[null,null,"Soggetti",null,"Proteomics"]
[{"$nulls":4},"Proteins - Analysis"]
[{"$nulls":4},"Molecular biology"]
[{"$nulls":4},"Electronic books."]
[null,null,"Lingua di pubblicazione",null,"Inglese"]
[null,null,"Formato",null,"Materiale a stampa"]
[null,null,null,"Livello bibliografico","Monografia"]
[null,null,null,"Note generali","Description based upon print version of record."]
[null,null,null,"Nota di bibliografia","Includes bibliographical references and index."]
[null,null,"Nota di contenuto",null,"Proteomics in Practice; Contents; Preface; Foreword; Abbreviations, Symbols, Units; Introduction; 1 History; 2 Critical Points; 2.1 Challenges of the Protein Samples; 2.2 Challenges of the Analysis Systems; 3 Proteomics Strategies; 3.1 Proteome Mapping; 3.2 Differential Analysis; 3.3 Time Point Experiments; 3.4 Verification of Targets or Biomarkers; 3.5 Integration of Results into Biological Context; 3.6 Systems Biology; 4 Concept of Experimental Planning; 4.1 Biological Replicates; 4.2 Pooling of Samples: Yes or No?; 4.3 Pre-fractionation of Samples: Yes or No?"]
[{"$nulls":4},"4.4 Which is the Best Workflow to Start With?Part I: Proteomics Technology; 1 Electrophoretic Techniques; 1.1 The Principle of Electrophoresis and Some Methodological Background; 1.1.1 Free Flow Electrophoretic Methods; 1.1.2 Gels for Electrophoretic Techniques; 1.1.3 Electroendosmosis Effects; 1.2 Polyacrylamide Gel Electrophoresis; 1.2.1 The Polyacrylamide Gel; 1.2.2 SDS Polyacrylamide Gel Electrophoresis; 1.2.3 Blue Native Electrophoresis; 1.2.4 Cationic Detergent Electrophoresis; 1.3 Blotting; 1.3.1 Electrophoretic Transfer; "]
[{"$nulls":4},"1.3.2 Protein Detection on the Membrane"]
[{"$nulls":4},"1.4 Isoelectric Focusing1.4.1 Theoretical Background; 1.4.2 Preparation of IEF Gels; 1.4.3 Isoelectric Focusing in Proteomics; 1.5 Two-dimensional Electrophoresis; 1.5.1 Sample Preparation; 1.5.2 Pre-labeling of Proteins for Difference Gel Electrophoresis; 1.5.3 First Dimension: Isoelectric Focusing in IPG Strips; 1.5.4 Second Dimension: SDS Electrophoresis; 1.5.5 Detection of Protein Spots; 1.6 Image Analysis; 1.6.1 Image Acquisition; 1.6.2 Image Analysis and Evaluation; 1.6.3 Use of 2-D Electrophoresis Data; 1.7 Spot Handling; 1.7.1 Spot Picking; 1.7.2 Protein Cleavage"]
[{"$nulls":4},"2 Liquid Chromatography Techniques2.1 Basic Principles of Important Liquid Chromatography Techniques; 2.1.1 Ion Exchange Chromatography; 2.1.2 Reversed Phase Chromatography; 2.1.3 Affinity Chromatography; 2.1.4 Gel Filtration; 2.2 Strategic Approach and General Applicability; 2.3 Liquid Chromatography Techniques and Applications in Proteome Analysis; 2.3.1 Peptide Separation; 2.3.2 2DLC Peptide Separation; 2.3.3 Affinity Chromatography and LC-MS/MS; 2.3.4 Protein Pre-fractionation; 2.4 Practical Considerations and Application of LC-based Protein Pre-fractionation"]
[{"$nulls":4},"2.4.1 Sample Extraction and Preparation2.4.2 Experimental Setup; 2.4.3 Ion Exchange Chromatography and Protein Pre-fractionation; 2.4.4 Reversed Phase Chromatography and Protein Pre-fractionation; 2.4.5 Fraction Size and Number of Fractions; 2.5 Critical Review and Outlook; 3 Mass Spectrometry; 3.1 Ionization; 3.1.1 Matrix Assisted Laser Desorption Ionization; 3.1.2 Electrospray Ionization; 3.2 Ion Separation; 3.2.1 Time-of-Flight Analyzer; 3.2.2 Triple Quadrupole Analyzer; 3.2.3 Quadrupole Ion Trap; 3.2.4 Quadrupole Time-of-Flight; 3.2.5 Hybrid Triple Quadrupole Linear Ion Trap"]
[{"$nulls":4},"3.2.6 TOF/TOF Analyzer"]
[null,null,null,"Sommario/riassunto","Still the only concise practical guide to laboratory experiments in proteomics, this new edition now also covers DIGE technology and liquid-chromatography, while the troubleshooting section has been considerably extended. Adopting a practical approach, the authors present the relevant techniques and explain the route to successful experimental design and optimal method selection. They cover such electrophoretic techniques as isoelectric focusing, SDS page, 2-D page, and DIGE, as well as liquid-chromatography techniques, such as ion exchange, affinity chromatography and reversed-phase HPLC."]
[null,"2.","Record Nr.",null,"UNINA9910794133503321"]
[null,null,"Autore",null,"Lancaster Rosemary"]
[null,null,"Titolo",null,"Women writing on the French Riviera : travellers and trendsetters, 1870-1970 / / by Rosemary Lancaster"]
[null,null,"Pubbl/distr/stampa",null,"Boston, Massachusetts : , : Brill Rodopi, , 2020"]
[null,null,"ISBN",null,"90-04-43392-9"]
[null,null,"Descrizione fisica",null,"1 online resource (287 pages) : illustrations"]
[null,null,"Collana",null,"Faux titre, , 0167-9392 ; ; volume 442"]
[null,null,"Disciplina",null,"840.9/9287"]
[null,null,"Soggetti",null,"Literary landmarks - France - Riviera"]
[{"$nulls":4},"Women authors, American - Homes and haunts - France - Riviera"]
[{"$nulls":4},"Women authors, English - Homes and haunts - France - Riviera"]
[{"$nulls":4},"Women authors, French - Homes and haunts - France - Riviera"]
[{"$nulls":4},"Women authors, Russian - Homes and haunts - France - Riviera"]
[{"$nulls":4},"Riviera (France) Intellectual life 20th century"]
[{"$nulls":4},"Riviera (France) Intellectual life 19th century"]
[{"$nulls":4},"Riviera (France) Biography"]
[null,null,"Lingua di pubblicazione",null,"Inglese"]
[null,null,"Formato",null,"Materiale a stampa"]
[null,null,null,"Livello bibliografico","Monografia"]
[null,null,null,"Nota di bibliografia","Includes bibliographical references (pages [263]-271) and index."]
[null,null,null,"Sommario/riassunto","\"Destination for artists and convalescents, playground of the rich, site of foreign allure, the French Riviera has long attracted visitors to its shores. Ranging through the late nineteenth century, the Belle Epoque, the 'roaring twenties', and the emancipatory post-war years, Rosemary Lancaster highlights the contributions of nine remarkable women to the cultural identity of the Riviera in its seminal rise to fame. Embracing an array of genres, she gives new focus to feminine writings never previously brought together, nor as richly critically explored. Fiction, memoir, diary, letters, even cookbooks and choreographies provide compelling evidence of the innovativeness of women who seized the challenges and opportunities of their travels in a century of radical social and artistic change\"--"]