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[null,"1.","Record Nr.",null,"UNINA9910132278903321"]
[null,null,"Autore",null,"Gerczei Timea"]
[null,null,"Titolo",null,"Biochemistry laboratory manual for undergraduates : an inquiry-based approach / / Timea Gerczei, Scott Pattison; managing editor, Anna Rulka"]
[null,null,"Pubbl/distr/stampa",null,"Warsaw, [Poland] ; ; Berlin, [Germany] : , : De Gruyter Open, , 2014"]
[{"$nulls":4},"©2014"]
[null,null,"ISBN",null,"1-5231-0070-2"]
[{"$nulls":4},"3-11-042625-0"]
[{"$nulls":4},"3-11-041133-4"]
[null,null,"Descrizione fisica",null,"1 online resource (186 pages) : illustrations, (some colour)"]
[null,null,"Disciplina",null,"572.078"]
[null,null,"Soggetti",null,"Biochemistry"]
[{"$nulls":4},"Molecular biology"]
[{"$nulls":4},"Drug resistance in microorganisms"]
[null,null,"Lingua di pubblicazione",null,"Inglese"]
[null,null,"Formato",null,"Materiale a stampa"]
[null,null,null,"Livello bibliografico","Monografia"]
[null,null,null,"Note generali","Bibliographic Level Mode of Issuance: Monograph"]
[null,null,"Nota di contenuto",null,"Front matter -- Preface -- Contents -- 1 Introducing the Bacterial Antibiotic Sensor Mini Project -- 2 Identifying Conserved Elements in the Toxin Sensor and Designing Mutants to Test Whether They are Important for Function -- 3 Designing Primers for Site-Directed Mutagenesis -- 4 Performing Site-Directed Mutagenesis -- 5 Purifying Mutant Toxin Sensor DNA from Bacterial Cells and Evaluating its Quality Using Agarose Gel Electrophoresis and UV Spectroscopy -- 6 Preparing DNA Template for Mutant RNA Sensor Synthesis Using a Restriction Endonuclease -- 7 Synthesizing the ykkCD Mutant Toxin Sensor RNA in vitro -- 8 Purifying the ykkCD Mutant Toxin Sensor RNA and Evaluating its Purity Using Denaturing PAGE and UV spectrometry -- 9 Evaluating the Ability of the ykkCD Toxin Sensor to Recognize the Antibiotic Tetracycline Using Fluorescent Quenching -- 10 Evaluating Antibiotic Binding to Blood Serum Albumin Using Fluorescence Spectroscopy -- 11 Understanding the Importance of Buffers in Biological Systems -- 12 Molecular Visualization of an Enzyme, Acetylcholinesterase -- 13 Determining the Efficiency of the Enzyme Acetylcholine Esterase Using Steady-State Kinetic Experiment -- 14 Separation of the Phosphatidylcholines Using Reverse Phase HPLC -- List of Figures"]
[null,null,null,"Sommario/riassunto","Biochemistry laboratory manual for undergraduates - an inquiry based "]
[{"$nulls":4},"approach by Gerczei and Pattison is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology while incorporating the blossoming field of bioinformatics. The novelty of this manual is the incorporation of a student-driven real real-life research project into the undergraduate curriculum. Since students test their own mutant design, even the most experienced students remain engaged with the process, while the less experienced ones get their first taste of biochemistry research. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic."]
[null,"2.","Record Nr.",null,"UNINA9910786083403321"]
[null,null,"Autore",null,"McCool Daniel"]
[null,null,"Titolo",null,"River Republic [[electronic resource] ] : The Fall and Rise of America's Rivers"]
[null,null,"Pubbl/distr/stampa",null,"New York, : Columbia University Press, 2012"]
[null,null,"Descrizione fisica",null,"1 online resource (655 p.)"]
[null,null,"Disciplina",null,"333.91620973"]
[null,null,"Soggetti",null,"Rivers - Environmental aspects - United States - History"]
[{"$nulls":4},"Rivers -- Environmental aspects -- United States -- History"]
[{"$nulls":4},"Stream conservation - United States"]
[{"$nulls":4},"Stream conservation -- United States"]
[{"$nulls":4},"Stream restoration - United States"]
[{"$nulls":4},"Stream restoration -- United States"]
[{"$nulls":4},"Water - Pollution - United States - History"]
[{"$nulls":4},"Water -- Pollution -- United States -- History"]
[null,null,"Lingua di pubblicazione",null,"Inglese"]
[null,null,"Formato",null,"Materiale a stampa"]
[null,null,null,"Livello bibliografico","Monografia"]
[null,null,null,"Note generali","Description based upon print version of record."]
[null,null,"Nota di contenuto",null,"Cover; Half title; Title; Copyright; Contents; Preface; Acknowledgments; Green River; Map: Selected Sites; Part I: The Fall; 1. Crumbling Edifice; The Disjuncture; Instigators; The Glen; Water Hubris; 2. Planters, Sawyers, and Snags: The U.S. Army Corps of Engineers; \"Building Strong\"; Not Just Peanuts; Rusting Rebar; The Big Boo-Boo; Under Siege; The Invitation; 3. The Manless Land: The Bureau of Reclamation; Watery Science; The Inland Empire and the Fifty Princesses; Dinosaur; Dominy; Sugar City; Turning the Corner; Blueprint for Reform; The Pendulum and the Rogue; Water 2025"]
[{"$nulls":4},"Heritage of ConflictPart II: Dismemberment; 4. Handout Horticulture: Farming and the Feds; The Agro-industrial Complex; Fish Traps; Dying 4 Water; Crops, Capitalism, and Context; 5. Falling Waters: Hydropower and Renewable Energy; Smolts and Volts; The Scenic and the Sacred; Bucket of Ashes; 6. Rivers Into Waterways: Barging, Locks, and Dams; Tallstacks; Cooking the Data; Dinner Buckets and Party Barges; A National Duty; 7. Black Water Rising: The Myth of Flood Control; The Storm of Good Friday; Galloway; House-a-Fire; Coon-Ass; The House of Death Floats By"]
[{"$nulls":4},"8. Downstream Dilemma: Water PollutionToxics Run Through It; Song "]
[{"$nulls":4},"of the Chattahoochee; Too Many Federal Toilets, Not Enough Senators; Superfund River; Pure River of Water of Fife; Part III: Resurrection; 9. River City: Urban Riverscapes; Belle Isle; River of the Angels; River of Democracy and the Emerald Necklace; A Great Multitude of Fish; The Pleasantness of the River; 10. Net Losses: Habitat and Endangered Species; Alphabet Gumbo; God Bless America; Ten Thousand Years of Fishing; A Foot Deep with Fish; The Other Side of the River; Show Me the Money"]
[{"$nulls":4},"11. Playground on the Move: River RecreationMoving-Water Recreation; Agents of Change; Lunkers and Extreme Instant Shade; Water Warriors; 12. The River Commons; Kitty Clyde's Sister; Black-Side Up; The Katrina Syndrome; Amenity Rivers; Instigators Redux; Lotic Dreams; Notes; Index"]
[null,null,null,"Sommario/riassunto","Daniel McCool not only chronicles the history of water development agencies in America and the way in which special interests have abused rather than preserved the country's rivers. He also narrates the second, brighter act in this ongoing story: the surging, grassroots movement to bring these rivers back to life and ensure they remain pristine for future generations.The culmination of ten years of research and observation, McCool's book confirms the surprising news that America's rivers are indeed returning to a healthier, free-flowing condition. The politics of river restoration"]