{"text_format":"2","workbook":"xls","n_sheets":1,"sheets":["report2"],"not_rendered":{"charts":0,"images":0,"macros":0,"external_links":1,"defined_names":1,"comments":0,"pivot_tables":0},"unopened":[],"bounds":{"document_bytes":16777216,"sheet_cells":100000,"run_cost":"one","rows_first":2000,"rows_last":500,"rows_between":2000,"halvings":0,"cell_chars":16384,"cell_head_chars":4096},"cells_cut":0,"cells_raw":0,"read":"whole","date_system":"1900","formulas":"cached_only"}

{"sheet":"report2","index":0,"n_rows":25,"n_cols":5,"rows_kept":25,"cut":false,"hidden":false,"merged":[[0,2,0,3],[0,4,0,5],[1,2,1,3],[1,4,1,5],[2,2,2,3],[2,4,2,5],[3,2,3,3],[4,2,4,3],[7,2,7,3],[8,2,8,3],[9,2,9,3],[11,2,11,3],[13,2,13,3],[14,2,14,3],[18,2,18,3]],"flags":["formulas_cached_only"]}
[null,"1.","Record Nr.",null,"UNINA9910784529603321"]
[null,null,"Autore",null,"Becker Jeffrey M"]
[null,null,"Titolo",null,"Biotechnology [[electronic resource] ] : a laboratory course / / Jeffrey M. Becker, Guy A. Caldwell, Eve Ann Zachgo"]
[null,null,"Pubbl/distr/stampa",null,"San Diego, : Academic Press, c1996"]
[null,null,"ISBN",null,"1-281-18650-3"]
[{"$nulls":4},"9786611186500"]
[{"$nulls":4},"0-08-052819-8"]
[null,null,"Edizione",null,"[2nd ed.]"]
[null,null,"Descrizione fisica",null,"1 online resource (283 p.)"]
[null,null,"Disciplina",null,"660.6"]
[{"$nulls":4},"660/.6/078 20"]
[null,null,"Soggetti",null,"Biochemical engineering"]
[{"$nulls":4},"Biotechnology"]
[null,null,"Lingua di pubblicazione",null,"Inglese"]
[null,null,"Formato",null,"Materiale a stampa"]
[null,null,null,"Livello bibliografico","Monografia"]
[null,null,null,"Note generali","Description based upon print version of record."]
[null,null,null,"Nota di bibliografia","Includes bibliographical references and index."]
[null,null,"Nota di contenuto",null,"Front Cover; Biotechnology: A Laboratory Course; Copyright Page; Contents; Preface to the Second Edition; Preface to the First Edition; Acknowledgments; Suggested Schedule for Exercises; Introductory Notes: Record Keeping and Safety Rules; Format of Student Laboratory Records; The Ten Commandments of Record Keeping; Safety Rules in the Laboratory; Exercise 1. Aseptic Technique and Establishing Pure Cultures: The Streak Plate and Culture Transfer; Exercise 2. Preparation of Culture Media; Exercise 3. The Growth Curve; Exercise 4. Isolation of Plasmid DNA from Escherichia coli: The Mini-Prep"]
[{"$nulls":4},"Exercise 5. Purification, Concentration, and Quantitation of DNAExercise 6. Large-Scale Isolation of Plasmid DNA by Column Chromatography; Exercise 7. Amplification of a lacZ Gene Fragment by the Polymerase Chain Reaction; Exercise 8. Restriction Digestion and Agarose Gel Electrophoresis; Exercise 9. Southern Transfer; Exercise 10. Preparation, Purification, and Hybridization of Probe; Exercise 11. Transformation of Saccharomyces cerevisiae; Exercise 12. Isolation of Plasmid from Yeast and Escherichia coil Transformation; Exercise 13. Protein Assays"]
[{"$nulls":4},"Exercise 14. Qualitative Assay for b-Galactosidase in Yeast ColoniesExercise 15. Determination of b-Galactosidase in Permeabilized Yeast Cells; Exercise 16. Assay of b-Galactosidase in Cell Extracts; Exercise 17. b-Galactosidase Purification; Exercise 18. "]
[{"$nulls":4},"Western Blot: Probe of Protein Blot with Antibody to b-Galactosidase; Appendix 1. Alternative Protocols and Experiments; Exercise 1A Isolation and Characterization of Auxotrophic Yeast Mutants; Exercise 2A Measurement of pH; Exercise 3A Use of the Spectrophotometer; Exercise 6A Isolation of Plasmid DNA: The Maxi-Prep"]
[{"$nulls":4},"Exercise 10A Colony HybridizationAppendix 2. Buffer Solutions; Appendix 3. Preparation of Buffers and Solutions; Appendix 4. Properties of Some Common Concentrated Acids and Bases; Appendix 5. Use of Micropipettors; Appendix 6. Safe Handling of Microorganisms; Appendix 7. List of Cultures; Appendix 8. Storage of Cultures and DNA; Appendix 9. Sterilization Methods; Appendix 10. Preparation of Stock Solutions for Culture Media; Appendix 11. Growth in Liquid Medium; Appendix 12. Determination of Viable Cells; Appendix 13. Determination of Cell Mass; Appendix 14. Determination of Cell Number"]
[{"$nulls":4},"Appendix 15. Nomenclature of StrainsAppendix 16. Glassware and Plasticware; Appendix 17. Preparation of Tris and EDTA; Appendix 18. Basic Rules for Handling Enzymes; Appendix 19. Effects of Common Contaminants on Protein Assays; Appendix 20. Manufacturers' and Distributors' Addresses; Appendix 21. Surfing the Bionet: World Wide Web Addresses; Glossary; Index"]
[null,null,null,"Sommario/riassunto","The objectives of this Second Edition of Biotechnology: A Laboratory Course remain unchanged: to create a text that consists of a series of laboratory exercises that integrate molecular biology with protein biochemistry techniques while providing a continuum of experiments. The course begins with basic techniques and culminates in the utilization of previously acquired technical experience and experimental material. Two organisms, Sacchaomyces cerevisiae and Escherichia coli, a single plasmid, and a single enzyme are the experimental material, yet the procedures and"]