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[null,"1.","Record Nr.",null,"UNINA9910780728103321"]
[null,null,"Autore",null,"Lim Chang Kee"]
[null,null,"Titolo",null,"High-performance liquid chromatography and mass spectrometry of porphyrins, chlorophylls and bilins [[electronic resource] /] / Chang Kee Lim"]
[null,null,"Pubbl/distr/stampa",null,"River Edge, N.J., : World Scientific, 2010"]
[null,null,"ISBN",null,"1-282-75824-1"]
[{"$nulls":4},"9786612758249"]
[{"$nulls":4},"981-4277-19-3"]
[null,null,"Descrizione fisica",null,"1 online resource (245 p.)"]
[null,null,"Collana",null,"Methods in chromatography ; ; v. 2"]
[null,null,"Disciplina",null,"547/.593"]
[null,null,"Soggetti",null,"Porphyrins"]
[{"$nulls":4},"Chlorophyll"]
[{"$nulls":4},"Tetrapyrroles"]
[{"$nulls":4},"High performance liquid chromatography"]
[{"$nulls":4},"Mass spectrometry"]
[null,null,"Lingua di pubblicazione",null,"Inglese"]
[null,null,"Formato",null,"Materiale a stampa"]
[null,null,null,"Livello bibliografico","Monografia"]
[null,null,null,"Note generali","Description based upon print version of record."]
[null,null,null,"Nota di bibliografia","Includes bibliographical references and index."]
[null,null,"Nota di contenuto",null,"Contents; Preface; Chapter 1. Structure, Distribution, Biosynthesis and Function; 1.1. Introduction; 1.2. Nomenclature; 1.3. Biosynthesis of Porphyrins, Haem and Chlorophyll; 1.4. Biosynthesis of Bilins in Animals and Plants; 1.4.1. Degradation of haem to bile pigments; 1.4.2. Biosynthesis of bilins in plants, algae and cyanobacteria; 1.4.3. Degradation of chlorophyll in senescent higher plants; 1.5. Function of Porphyrins and Other Tetrapyrroles; References; Chapter 2. High-Performance Liquid Chromatography of Porphyrins; 2.1. Introduction; 2.2. HPLC of Porphyrin Methyl Esters"]
[{"$nulls":4},"2.3. HPLC of Porphyrin Free Acids2.3.1. Stationary phases for reversed-phase HPLC of porphyrins; 2.3.2. Mobile phases for reversed-phase HPLC of porphyrins; 2.3.3. Procedure for optimisation of porphyrin separation by reversed-phase HPLC; 2.3.4. Retention behaviour of porphyrins in reversed-phase HPLC; 2.4 HPLC of Porphyrinogens; References; Chapter 3. Mass Spectrometry of Porphyrins; 3.1. Introduction; 3.2. Fast Atom Bombardment (FAB) Mass Spectrometry of Porphyrins; 3.3. Laser Desorption/Ionisation Time-of-Flight (LDI-TOF) Mass Spectrometry of Porphyrins"]
[{"$nulls":4},"3.4 Electrospray Ionisation Mass Spectrometry (ESI-MS) and HPLC/ESI-"]
[{"$nulls":4},"MS of Porphyrins3.4.1. HPLC/ESI-MS of porphyrins; 3.4.2. ESI-MS/MS fragmentation of porphyrins; 3.4.2.1. ESI-MS/MS product ion spectrum and fragmentation pathways of uroporphyrin; 3.4.3. ESI-MS/MS fragmentation of hydroxyuroporphyrins; 3.4.3.1. ESI-MS/MS product ion spectrum and fragmentation pathways of meso-hydroxyuroporphyrin; 3.4.3.2. ESI-MS/MS product ion spectrum of hydroxyacetic acid uroporphyrin; 3.4.3.3. ESI-MS/MS product ion spectrum of α-hydroxypropionic acid uroporphyrin"]
[{"$nulls":4},"3.4.3.4. ESI-MS/MS product ion spectrum and fragmentation pathways of β-hydroxypropionic acid uroporphyrin3.4.3.5. ESI-MS/MS product ion spectra and fragmentation pathways of cis- and transhydroxyspirolactoneurochlorins; 3.4.3.6. ESI-MS/MS product ion spectra and fragmentation pathways of cis- and transdihydroxyurochlorins; 3.4.3.7. Characteristic ESI-MS/MS product ions of hydroxyuroporphyrins; 3.4.4. ESI-MS/MS product ion spectra of ketoacid uroporphyrins; 3.4.5. ESI-MS/MS of heptacarboxylic porphyrin; 3.4.6. ESI-MS/MS of hydroxyheptacarboxylic porphyrins"]
[{"$nulls":4},"3.4.7. ESI-MS/MS of ketoacid heptacarboxylic porphyrins and formyl heptacarboxylic porphyrin3.4.8. ESI-MS/MS product ion spectrum and fragmentation pattern of coproporphyrin; 3.4.9. ESI-MS/MS of hydroxymethylcoproporphyrin; 3.4.10. ESI-MS/MS of β-ketopropionic acid  coproporphyrin and formylcoproporphyrin; 3.4.11. ESI-MS/MS product ion spectrum and fragmentation pattern of protoporphyrin; 3.4.12. HPLC/ESI-MS and MS/MS of meso-tetraphenylporphyrin derivatives; References; Chapter 4. Porphyrin Profiles in Blood, Urine and Faeces by HPLC and HPLC/ESI-MS; 4.1. Introduction"]
[{"$nulls":4},"4.2. Sample Collection and Preparation"]
[null,null,null,"Sommario/riassunto","The porphyrins, chlorophylls, bilins and related tetrapyrroles are vital for all living organisms. Natural and synthetic tetrapyrroles are used extensively in foods, cosmetics, biotechnology, pharmaceuticals, diagnostics and medicine. Methods for their separation and characterization therefore, have a very wide area of applications. Yet, there is a dearth of books dedicated to HPLC and HPLC/MS of tetrapyrroles. Lim addresses this problem admirably by providing practical HPLC and HPLC/MS protocols coupled with in-depth chromatographic and mass spectrometric reference data. These are invaluable"]