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[null,"1.","Record Nr.",null,"UNINA9910707096203321"]
[null,null,"Autore",null,"Volkert Richard A."]
[null,null,"Titolo",null,"Geochemistry and stratigraphic relations of Middle Proterozoic rocks of the New Jersey Highlands / / by Richard A. Volkert and Avery Ala Drake, Jr"]
[null,null,"Pubbl/distr/stampa",null,"Washington : , : United States Government Printing Office"]
[{"$nulls":4},"Reston, Va. : , : U.S. Geological Survey, , 1999"]
[null,null,"Descrizione fisica",null,"v, 77 pages : illustrations ; ; 28 cm"]
[null,null,"Collana",null,"Geologic studies in New Jersey and eastern Pennsylvania ; ; C"]
[{"$nulls":4},"U.S. Geological Survey professional paper ; ; 1565"]
[null,null,"Disciplina",null,"557.49 s"]
[{"$nulls":4},"551.7/15/09749"]
[null,null,"Soggetti",null,"Geology, Stratigraphic - Proterozoic"]
[{"$nulls":4},"Geology - New Jersey - New Jersey Highlands"]
[{"$nulls":4},"Geochemistry - New Jersey - New Jersey Highlands"]
[{"$nulls":4},"Geochemistry"]
[{"$nulls":4},"Geology"]
[{"$nulls":4},"Geology, Stratigraphic"]
[{"$nulls":4},"Proterozoic Geologic Period"]
[{"$nulls":4},"New Jersey New Jersey Highlands"]
[null,null,"Lingua di pubblicazione",null,"Inglese"]
[null,null,"Formato",null,"Materiale a stampa"]
[null,null,null,"Livello bibliografico","Monografia"]
[null,null,null,"Note generali","\"Prepared in cooperation with the New Jersey Geological Survey.\""]
[{"$nulls":4},"\"New Jersey Highlands Middle Proterozoic rocks include sodic basement rocks of the Losee Metamorphic Suite and an unconformably overlying metasedimentary sequence intruded by synkinematic granitoids (~1,090 Ma) and postkinematic granite (1,020 Ma).\""]
[null,null,null,"Nota di bibliografia","Includes bibliographical references (page 29-31)."]
[null,"2.","Record Nr.",null,"UNINA9910831037603321"]
[null,null,"Titolo",null,"Detection of highly dangerous pathogens [[electronic resource] ] : microarray methods for BSL 3 and BSL 4 agents / / edited by Tanja Kostic, Patrick Butaye, and Jacques Schrenzel"]
[null,null,"Pubbl/distr/stampa",null,"Weinheim, : Wiley-VCH, 2009"]
[null,null,"ISBN",null,"1-282-29205-6"]
[{"$nulls":4},"9786612292057"]
[{"$nulls":4},"3-527-62668-9"]
[{"$nulls":4},"3-527-62669-7"]
[null,null,"Descrizione fisica",null,"1 online resource (193 p.)"]
[null,null,"Disciplina",null,"579.165"]
[null,null,"Soggetti",null,"Pathogenic microorganisms - Detection"]
[{"$nulls":4},"DNA microarrays"]
[null,null,"Lingua di pubblicazione",null,"Inglese"]
[null,null,"Formato",null,"Materiale a stampa"]
[null,null,null,"Livello bibliografico","Monografia"]
[null,null,null,"Note generali","Description based upon print version of record."]
[null,null,null,"Nota di bibliografia","Includes bibliographical references and index."]
[null,null,"Nota di contenuto",null,"Detection of Highly Dangerous Pathogens: Microarray Methods for the Detection of BSL 3 and BSL 4 Agents; Contents; This Publication is Supported by COST; Preface; List of Contributors; 1 Introduction to Microarray-Based Detection Methods; 1.1 Introduction to Microarray Technology; 1.2 Technical Aspects of Microarray Technology; 1.2.1 Probes; 1.2.1.1 Genome Fragments; 1.2.1.2 PCR Products; 1.2.1.3 Oligonucleotide Probes; 1.2.2 Substrates for Printing; 1.2.2.1 Slides with Poly-L-lysine Coating; 1.2.2.2 Slides with Amino Silane Coating; 1.2.2.3 Slides with Aldehyde Coating"]
[{"$nulls":4},"1.2.2.4 Slides with Epoxy Coating1.2.2.5 Proprietary Surface Chemistries; 1.2.2.6 Probe Spacers; 1.2.3 Targets for Microarray Analysis; 1.2.3.1 Target Amplifications and Sensitivity Issues; 1.2.3.2 Labeling of the Targets; 1.2.3.3 Hybridization and Wash Conditions; 1.2.4 Classical Commercially Available Microarray Formats; 1.2.4.1 Spotting Approaches; 1.2.4.2 In Situ Synthesis; 1.2.5 Alternative Methods for Improving Microarray-Based Detection Sensitivity; 1.2.5.1 Resonance-Light Scattering (RLS); 1.2.5.2 Planar-Waveguide Technology (PWT); 1.2.5.3 Liquid Arrays"]
[{"$nulls":4},"1.2.5.4 Three-Dimensional Microarray Formats1.2.6 Marker Genes Used on MDMs; 1.3 Analysis and Quality Control Aspects; 1.4 Applications of Microarray Technology in Microbial Diagnostics; 1.4.1 "]
[{"$nulls":4},"Gene Expression Studies; 1.4.2 Comparative Genomic Hybridization (CGH); 1.4.3 Generic or Universal Microarrays; 1.4.4 Microarrays for Sequence Analysis; 1.4.5 Microbial Diagnostic Microarrays (MDMs); 1.5 Further Developments and New Perspectives Regarding Array Sensitivity and Specificity; 1.6 Conclusions; References; Part I: Methods; 2 Long Oligonucleotide Microarray-Based Microbial Detection"]
[{"$nulls":4},"2.1 Introduction2.2 Method; 2.2.1 DNA Extraction; 2.2.2 Ф29 Amplification; 2.2.3 Klenow Amplification/Labeling; 2.2.4 Probe and Slide Preparation; 2.2.5 Slide Processing Protocol (for Amino Surfaces); 2.2.6 Hybridization and Slide Washing; 2.2.7 Comments; 2.3 Our Test System and Results; 2.4 Conclusions; References; 3 Sequence-Specific End-Labeling of Oligonucleotides; 3.1 Introduction; 3.2 Probe Design; 3.3 Slide Preparation (Spotting); 3.4 Slide Processing Protocol ( for Aldehyde Surfaces); 3.5 DNA Extraction and PCR Amplification of the Targeted Gene"]
[{"$nulls":4},"3.6 Shrimp Alkaline Phosphatase Treatment3.7 Labeling; 3.8 Hybridization and Slide Washing; 3.9 Data Analysis; 3.10 Costs; 3.11 Microarray for Detection of Pathogenic Bacteria; References; 4 Non-Cognate Approaches for Pathogen Detection on Microarrays; 4.1 Introduction; 4.2 Non-Cognate Hybridization System; 4.2.1 Concept; 4.2.2 Definition of the Optimal Probe Length; 4.2.3 Virtual Assessment of Array Performances (in Silico Experiments); 4.2.4 Array Manufacturing and Hybridization (Wet-Lab Experiments); 4.2.5 Analysis; 4.3 Perspectives; References; 5 Patterning Techniques for Array Platforms"]
[{"$nulls":4},"5.1 Introduction"]
[null,null,null,"Sommario/riassunto","Written by leading experts in the field as part of an interdisciplinary pan-European research program funded by the EU, the results provided in this booklet provide a unique and comprehensive overview of how microarray technology can be used in safely tracking the most highly dangerous pathogens. A must-have for public health agencies focused on bioterrorism as well as all laboratories working with BSL3 and/or BSL 4 agents."]