{"text_format":"2","workbook":"xls","n_sheets":1,"sheets":["report2"],"not_rendered":{"charts":0,"images":0,"macros":0,"external_links":1,"defined_names":1,"comments":0,"pivot_tables":0},"unopened":[],"bounds":{"document_bytes":16777216,"sheet_cells":100000,"run_cost":"one","rows_first":2000,"rows_last":500,"rows_between":2000,"halvings":0,"cell_chars":16384,"cell_head_chars":4096},"cells_cut":0,"cells_raw":0,"read":"whole","date_system":"1900","formulas":"cached_only"}

{"sheet":"report2","index":0,"n_rows":21,"n_cols":5,"rows_kept":21,"cut":false,"hidden":false,"merged":[[0,2,0,3],[0,4,0,5],[1,2,1,3],[1,4,1,5],[2,2,2,3],[4,2,4,3],[7,2,7,3],[8,2,8,3],[11,2,11,3],[12,2,12,3],[15,2,15,3]],"flags":["formulas_cached_only"]}
[null,"1.","Record Nr.",null,"UNINA9910590101003321"]
[null,null,"Titolo",null,"Molecular fluorescent sensors for cellular studies / / edited by Elizabeth J. New"]
[null,null,"Pubbl/distr/stampa",null,"Chichester, West Sussex : , : John Wiley & Sons, Incorporated, , 2022"]
[{"$nulls":4},"©2022"]
[null,null,"ISBN",null,"9781119749844"]
[{"$nulls":4},"1-119-74984-0"]
[{"$nulls":4},"1-119-74980-8"]
[null,null,"Descrizione fisica",null,"1 online resource (307 pages)"]
[null,null,"Soggetti",null,"Fluorescent probes"]
[{"$nulls":4},"Biosensors"]
[{"$nulls":4},"Fluorescence spectroscopy"]
[null,null,"Lingua di pubblicazione",null,"Inglese"]
[null,null,"Formato",null,"Materiale a stampa"]
[null,null,null,"Livello bibliografico","Monografia"]
[null,null,null,"Nota di bibliografia","Includes bibliographical references and index."]
[null,null,"Nota di contenuto",null,"Cover -- Title Page -- Copyright Page -- Contents -- List of Contributors -- Chapter 1 An Introduction to Small Molecule Fluorescent Sensors -- 1.1 What is Fluorescence? -- 1.2 Why Is Fluorescence Useful? -- 1.3 What Is a Fluorescent Sensor? -- 1.4 General Types of Fluorescent Sensors -- 1.5 Important Parameters -- 1.5.1 Excitation Maxima -- 1.5.2 Emission Maxima -- 1.5.3 Stokes Shift -- 1.5.4 Quantum Yield -- 1.5.5 Molar Extinction Coefficient -- 1.5.6 Brightness -- 1.5.7 Lifetime -- 1.5.8 Photobleaching -- 1.5.9 Anisotropy -- 1.5.10 Quenching -- 1.6 Fluorescence Mechanisms Used in Fluorescent Sensors -- 1.6.1 Photoinduced Electron Transfer -- 1.6.2 Internal Charge Transfer -- 1.6.3 Förster Resonance Energy Transfer -- 1.6.4 Through Bond Energy Transfer -- 1.6.5 Excited-State Intramolecular Proton Transfer -- 1.6.6 Aggregation-Induced Emission -- 1.6.7 Excimer Formation -- 1.7 Commonly Used Fluorophores -- 1.7.1 Fluorescein -- 1.7.2 Rhodamine -- 1.7.3 Coumarin -- 1.7.4 Naphthalimide -- 1.7.5 BODIPY (4,4-Difluoro4-bora-3a,4a-diaza-s-indacene) -- 1.7.6 Cyanine -- 1.8 Summary -- References -- Chapter 2 The Applications of Responsive Fluorescent Sensors to Biological Systems -- 2.1 Criteria for Biologically Relevant Fluorescent Sensors -- 2.2 Microscopy for Visualising Fluorescent Sensors -- 2.2.1 Important Considerations in Microscopy -- 2.2.2 Common Microscopy Techniques "]
[{"$nulls":4},"-- 2.3 Other Instrumental Techniques for Studying Cells Treated with Fluorescent Sensors -- 2.3.1 Flow Cytometry -- 2.3.2 Fluorescence Plate-readers -- 2.4 Biological Samples to Which Fluorescent Sensors Can Be Applied -- 2.4.1 Cultured Mammalian Cells -- 2.4.2 Bacteria -- 2.4.3 Plants -- 2.4.4 Multi-cellular Organisms -- 2.4.5 Towards In Vivo Imaging -- 2.5 Common Challenges and Misconceptions in the Applications of Fluorescent Sensors."]
[{"$nulls":4},"2.5.1 Important Considerations in Applying Sensors -- 2.5.2 Common Misconceptions About the Use of Sensors - The Bridge Between Multiple Disciplines -- 2.6 Conclusions -- References -- Chapter 3 Methods to Control the Subcellular Localisation of Fluorescent Sensors -- 3.1 Introduction -- 3.2 Targeting the Nucleus -- 3.3 Targeting Mitochondria -- 3.4 Targeting Lysosomes -- 3.5 Targeting Endosomes -- 3.6 Targeting Autophagic Compartments -- 3.7 Targeting Peroxisomes -- 3.8 Targeting the Endoplasmic Reticulum -- 3.9 Targeting the Golgi Apparatus -- 3.10 Targeting Lipid Droplets -- 3.11 Targeting the Plasma Membrane -- 3.12 Targeting the Cytoskeleton -- 3.13 Targeting the Cytosol -- 3.14 Trapping and Accumulation of Sensors -- References -- Chapter 4 Recognition-based Sensors for Cellular Imaging -- 4.1 Considerations for Recognition-based Sensing -- 4.1.1 Receptor-Analyte Recognition and Binding Affinity -- 4.1.2 Key Considerations to Enhance Selective Receptor to Analyte Recognition -- 4.2 Recognition-based Cation Sensing -- 4.2.1 Group I and II Metal Sensing -- 4.2.2 Essential Transition Metal Sensing -- 4.2.3 Toxic Metal Sensing -- 4.3 Recognition-based Anion Sensing -- 4.3.1 Anion Sensing Approaches -- 4.3.2 Halogen Ions Sensing -- 4.3.3 Inorganic Phosphates and Pyrophosphates -- 4.3.4 Bicarbonate, Hydrogen Sulfate, and Nitrate -- 4.4 Conclusions -- References -- Chapter 5 Activity-based Fluorescent Sensors and Their Applications in Biological Studies -- 5.1 Introduction -- 5.1.1 Design Principles -- 5.2 Oxidation Reactions for Sensing Oxidative Species -- 5.2.1 Fluorescent Sensors for Hydrogen Peroxide -- 5.2.2 Fluorescent Sensors for Peroxynitrite -- 5.2.3 Fluorescent Sensors for Hypochlorous Acid -- 5.2.4 Fluorescent Sensors for Nitric Oxide -- 5.2.5 Fluorescent Sensors for Singlet Oxygen -- 5.3 Reduction Reactions for Sensing Reductive Species."]
[{"$nulls":4},"5.3.1 Fluorescent Sensors for Hydrogen Sulfides -- 5.3.2 Fluorescent Sensors for Glutathione, Cysteine, and Homocysteine -- 5.3.3 Fluorescent Sensors for Selenocysteine -- 5.4 Reactions for Sensing Carbonyl Species -- 5.4.1 Fluorescent Sensors for Formaldehyde -- 5.4.2 Fluorescent Sensors for Methylglyoxal -- 5.5 Metal-mediated Reactions -- 5.6 Metal-sensing Reactions -- 5.7 Enzymatic Reactions -- 5.8 Reversible Reactions -- 5.8.1 Nucleophilic Conjugate Additions -- 5.8.2 Nucleophilic Addition -- 5.8.3 Imine Formation -- 5.8.4 Oxidation-Reduction Reactions -- 5.9 Analyte Regeneration -- 5.10 Summary -- References -- Chapter 6 Fluorescent Sensors of the Cellular Environment -- 6.1 Fluorescent Sensors for Polarity and Viscosity -- 6.1.1 The Biological Significance of Polarity and Viscosity -- 6.1.2 Twisted Intramolecular Charge Transfer as a Mechanism for Polarity and Viscosity Sensing -- 6.1.3 Polarity Sensors Based on Other Mechanisms -- 6.2 Fluorescent Sensors for pH -- 6.2.1 The Regulation of pH in Health and Disease -- 6.2.2 Considerations and Design Strategies for the Preparation of pH Sensors -- 6.2.3 Examples of pH Sensors -- 6.3 Fluorescent Redox Sensors for Biological Studies -- 6.3.1 The Regulation of Redox State in Health and Disease -- 6.3.2 Design Strategies of Fluorescent Redox Sensors and Key Examples -- 6.4 Conclusions -- References -- Chapter 7 Labelling Proteins and Biomolecules with Small Fluorescent Sensors -- "]
[{"$nulls":4},"7.1 Labelling Biomolecules in Cells with Fluorescent Sensors -- 7.2 Small-molecule Modifications and Bioorthogonal Reactions -- 7.2.1 Polar Ketone and Aldehyde Condensations -- 7.2.2 Azide Bioorthogonal Chemistry -- 7.2.3 Tetrazine Ligation -- 7.2.4 Commercial Fluorescent Labels -- 7.3 Short peptide Recognition Sequences -- 7.4 Fusion Protein Tagging Systems -- 7.4.1 FKBP Tag -- 7.4.2 eDHFR Tag -- 7.4.3 PYP Tag."]
[{"$nulls":4},"7.4.4 SNAP-Tag and CLIP-Tag -- 7.4.5 HaloTag -- 7.5 Enzymatic Modifications for Labelling Proteins -- 7.5.1 The LAP-tag System -- 7.5.2 Protein Trans-splicing -- 7.6 Future Developments -- References -- Chapter 8 Future Directions of Fluorescence Sensors for Cellular Studies -- 8.1 Fluorescence Lifetime Imaging Microscopy -- 8.1.1 Introduction -- 8.1.2 Advantages of Fluorescence Lifetime Imaging Microscopy -- 8.1.3 Examples of Sensors for FLIM -- 8.1.4 Future Directions -- 8.2 Near-infrared Sensors -- 8.2.1 Strategies to Make NIR Sensors -- 8.2.2 NIR Fluorophore Scaffolds -- 8.2.3 Future Directions -- 8.3 Dual-analyte Sensing -- 8.3.1 Introduction -- 8.3.2 Recognition-Based Dual-analyte Sensors -- 8.3.3 Activity-based Dual-analyte Sensors -- 8.3.4 Mixed Dual-analyte Sensors -- 8.3.5 Sequence-specific Reactions -- 8.3.6 Conclusions and Future Directions -- 8.4 Super-resolution Microscopy -- 8.4.1 Introduction -- 8.4.2 Super-resolution Microscopy Techniques -- 8.4.3 Considerations for Use of Super Resolution Microscopy -- 8.4.4 Fluorescent Sensors for Super-resolution Microscopy -- 8.4.5 Future Directions -- 8.5 Multimodality -- 8.5.1 Introduction -- 8.5.2 Radioisotope Techniques -- 8.5.3 Computed Tomography -- 8.5.4 Magnetic Resonance Imaging -- 8.5.5 Photoacoustic Imaging -- 8.5.6 Vibrational Spectroscopy -- 8.5.7 Synchrotron X-ray Techniques -- 8.5.8 Mass Spectrometry -- 8.5.9 Electron Microscopy -- 8.5.10 Three or More Modalities -- 8.5.11 Future Directions -- References -- Index -- EULA."]