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[null,"1.","Record Nr.",null,"UNINA9910139923703321"]
[null,null,"Autore",null,"Gjerde Douglas T"]
[null,null,"Titolo",null,"RNA purification and analysis [[electronic resource] ] : sample preparation, extraction, chromatography / / by Douglas T. Gjerde, Lee Hoang and David Hornby"]
[null,null,"Pubbl/distr/stampa",null,"Weinheim, : Wiley-VCH"]
[{"$nulls":4},"Chichester, : John Wiley [distributor], c2009"]
[null,null,"ISBN",null,"1-282-68979-7"]
[{"$nulls":4},"9786612689796"]
[{"$nulls":4},"3-527-62719-7"]
[{"$nulls":4},"3-527-62720-0"]
[null,null,"Descrizione fisica",null,"1 online resource (209 p.)"]
[null,null,"Disciplina",null,"572.88"]
[{"$nulls":4},"572.8836"]
[{"$nulls":4},"574.87/3283"]
[null,null,"Soggetti",null,"RNA - Analysis"]
[{"$nulls":4},"RNA - Purification"]
[null,null,"Lingua di pubblicazione",null,"Inglese"]
[null,null,"Formato",null,"Materiale a stampa"]
[null,null,null,"Livello bibliografico","Monografia"]
[null,null,null,"Note generali","Description based upon print version of record."]
[null,null,null,"Nota di bibliografia","Includes bibliographical references and index."]
[null,null,"Nota di contenuto",null,"RNA Purification and Analysis: Sample Preparation, Extraction, Chromatography; Contents; Preface; Acknowledgments; 1: RNA Extraction, Separation, and Analysis; 1.1 The Need to Be Able to Extract, Manipulate, and Analyze RNA; 1.2 Using Chemical Tools to Solve the Problem of Analysis of Biological Processes; 1.3 The Principle of Chromatography and Solid-Phase Extraction; 1.3.1 Principle of Chromatography; 1.3.2 Mobile Phase Gradient Controls Elution; 1.3.3 Different Types of Column and Eluent Chemistries; 1.3.4 The Principle of Solid-Phase Extraction; 1.4 RNA Chromatography"]
[{"$nulls":4},"1.5 Enzymatic Treatment of RNA and Analysis1.5.1 Polyacrylamide Gel Electrophoresis; 1.5.2 RNA Structure Probing with Ribonuclease Enzymes; 1.6 Content and Organization of This Book; References; 2: Biological and Chemical RNA; 2.1 Why Classify RNA with Biology and Chemistry?; 2.1.1 Chemical Classification of RNA; 2.1.2 Biological Classification of RNA; 2.2 Prokaryotic Cellular RNA; 2.3 Prokaryote Sample Type; 2.3.1 Escherichia coli; 2.3.2 Other Bacteria; 2.4 Eukaryotic Cellular RNA; 2.5 Eukaryote Sample Type; 2.5.1 Yeast; 2.5.2 Other Fungi; 2.5.3 Simple Multicellular Organism"]
[{"$nulls":4},"2.5.4 Soft Animal2.5.5 Hard Animal; 2.5.6 Plant; 2.5.7 Cell Culture; 2.6 Other Samples; 2.6.1 Virus; 2.6.2 Soil and Rock; 2.7 Synthetic RNA; 2.7.1 Aptamers; 2.7.2 SELEX; 2.7.3 Short Hairpin RNAs; References; 3: RNA Separation: Substrates, Functional Groups, Mechanisms, and Control; 3.1 Solid-Phase Interaction; 3.1.1 Adsorption of Sample Compounds and Sample Matrix Compounds; 3.1.2 Roles of Solid-Phase Substrate and Functional Group; 3.1.3 Correlation of Interaction Type, Functional Group, and Substrate; 3.1.4 RNA Structure and Solid Surface Interaction"]
[{"$nulls":4},"3.2 The Solid-Phase Substrate and Attachment of Functional Groups3.2.1 Polymeric Resin Substrates; 3.2.2 Porous and Nonporous Polymeric Resins; 3.2.3 Monolith Polymeric Columns; 3.2.4 Functionalization of the Polymer; 3.2.5 Silica-Glass-Based Substrates; 3.2.6 Functionalization of Silica; 3.2.7 Agarose and Cellulose Affinity Substrates; 3.2.8 Dextran and Polyacrylamide Gel Filtration Substrates; 3.3 Reverse-Phase Ion-Pairing Separation Mechanism; 3.4 Ion-Exchange Separation Mechanism; 3.5 Chaotropic Denaturing Interaction Mechanism; 3.6 Hybridization; 3.6.1 SELEX; 3.7 Gel Filtration"]
[{"$nulls":4},"References4: RNA Extraction and Analysis; 4.1 Transcription; 4.1.1 RNA Catalysis; 4.1.2 RNA-Protein Complex Interactions; 4.1.3 Pre-mRNA Splicing; 4.2 Translation; 4.2.1 Post-Transcriptional Control of Eukaryotic Gene Expression; 4.3 Gene Regulation; 4.3.1 RNA Interference Pathway; 4.3.2 Micro RNAs and Their Role in Gene Regulation; 4.4 Use of siRNA to Investigate Gene Function; References; 5: RNA Chromatography; 5.1 Development of RNA Chromatography; 5.2 RNA Chromatography Instrumentation; 5.2.1 The Column Oven; 5.2.2 Ultraviolet (UV) and Fluorescence Detection; 5.2.3 Fragment Collection"]
[{"$nulls":4},"5.3 RNA Chromatography Conditions"]
[null,null,null,"Sommario/riassunto","This first book on the market covers the many new and important RNA species discovered over the past five years, explaining current methods for the enrichment, separation and purification of these novel RNAs.Building up from general principles of RNA biochemistry and biophysics, this book addresses the practical aspects relevant to the laboratory researcher throughout, while discussing the performance and potential problems of the methods discussed. An appendix contains a glossary with the important terms and techniques used in RNA analysis.By explaining the basic and working principle"]
[null,"2.","Record Nr.",null,"UNINA9910485011003321"]
[null,null,"Titolo",null,"Frontiers of Combining Systems : 9th International Symposium, FroCoS 2013, Nancy, France, September 18-20, 2013, Proceedings / / edited by Pascal Fontaine, Christophe Ringeissen, Renate Schmidt"]
[null,null,"Pubbl/distr/stampa",null,"Berlin, Heidelberg : , : Springer Berlin Heidelberg : , : Imprint : Springer, , 2013"]
[null,null,"ISBN",null,"3-642-40885-0"]
[null,null,"Edizione",null,"[1st ed. 2013.]"]
[null,null,"Descrizione fisica",null,"1 online resource (XII, 359 p. 35 illus.)"]
[null,null,"Collana",null,"Lecture Notes in Artificial Intelligence, , 2945-9141 ; ; 8152"]
[null,null,"Disciplina",null,"006.3"]
[null,null,"Soggetti",null,"Artificial intelligence"]
[{"$nulls":4},"Machine theory"]
[{"$nulls":4},"Computer science"]
[{"$nulls":4},"Software engineering"]
[{"$nulls":4},"Algorithms"]
[{"$nulls":4},"Computer programming"]
[{"$nulls":4},"Artificial Intelligence"]
[{"$nulls":4},"Formal Languages and Automata Theory"]
[{"$nulls":4},"Computer Science Logic and Foundations of Programming"]
[{"$nulls":4},"Software Engineering"]
[{"$nulls":4},"Programming Techniques"]
[null,null,"Lingua di pubblicazione",null,"Inglese"]
[null,null,"Formato",null,"Materiale a stampa"]
[null,null,null,"Livello bibliografico","Monografia"]
[null,null,null,"Note generali","Bibliographic Level Mode of Issuance: Monograph"]
[null,null,"Nota di contenuto",null,"Invited Talk 1 -- MetiTarski’s Menagerie of Cooperating Systems -- Inductive Theorem Proving -- Combining Superposition and Induction: A Practical Realization -- Arrays and Memory Access Optimization -- Definability of Accelerated Relations in a Theory of Arrays and Its Applications -- Verification of Composed Array-Based Systems with Applications to Security-Aware Workflows -- Presburger Arithmetic in Memory Access Optimization for Data-Parallel Languages -- Approximation and Forgetting -- Roughening the EL Envelope -- Uniform Interpolation of ALC-Ontologies Using Fixpoints -- Abduction in Logic Programming as Second-Order Quantifier Elimination -- Invited Talk 2 -- Witness Runs for Counter Machines -- Temporal and Description Logic Techniques -- Decidability and Complexity via Mosaics of the Temporal Logic of the Lexicographic Products of "]
[{"$nulls":4},"Unbounded Dense Linear Orders -- Temporal Query Answering in the Description Logic DL-Lite -- Verification of Golog Programs over Description Logic Actions -- Invited Talk 3 -- Specification and Verification of Linear Dynamical Systems: Advances and Challenges -- Theorem Proving with Theories and Sorts -- Obtaining Finite Local Theory Axiomatizations via Saturation -- Non-cyclic Sorts for First-Order Satisfiability -- Detection of First Order Axiomatic Theories -- Mechanizing the Metatheory of Sledgehammer -- Invited Talk 4 -- From Resolution and DPLL to Solving Arithmetic Constraints -- Modal Logic and Description Logic -- Tableaux for Relation-Changing Modal Logics -- Computing Minimal Models Modulo Subset-Simulation for Modal Logics -- Hybrid Unification in the Description Logic EL -- Rewriting -- Disproving Confluence of Term Rewriting Systems by Interpretation and Ordering -- On Forward Closure and the Finite Variant Property -- Term Rewriting with Logical Constraints."]
[null,null,null,"Sommario/riassunto","This book constitutes the refereed proceedings of the 9th International Symposium on Frontiers of Combining Systems, FroCoS 2013, held in Nancy, France, in September 2013. The 20 revised full papers presented together with 4 invited papers were carefully reviewed and selected from 33 submissions. FroCoS'13 seeks to offer a common forum for research in the general area of combination,modularization and integration of systems, with emphasis on logic-based ones, and of their practical use.  Typical topics of interest include following subjects: combinations of logics such as combined predicate, temporal, modal or epistemic logics, combinations and modularity in ontologies, combination of decision, procedures, of satisfiability, procedures and of constraint solving techniques, combinations and modularity in term rewriting, integration of equational and other theories into deductive systems, combination of deduction systems and computer algebra, integration of data structures into constraint logic programming and deduction, and modularizing programs and specifications."]