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[null,"1.","Record Nr.",null,"UNINA9910483771803321"]
[null,null,"Autore",null,"Reneau Allyson"]
[null,null,"Titolo",null,"Moon first and Mars second : a practical approach to human space exploration / / Allyson Reneau"]
[null,null,"Pubbl/distr/stampa",null,"Cham, Switzerland : , : Springer, , [2021]"]
[{"$nulls":4},"Â©2021"]
[null,null,"ISBN",null,"3-030-54230-0"]
[null,null,"Edizione",null,"[1st ed. 2021.]"]
[null,null,"Descrizione fisica",null,"1 online resource (XVII, 104 p. 36 illus., 31 illus. in color.)"]
[null,null,"Collana",null,"SpringerBriefs in space development"]
[null,null,"Disciplina",null,"629.45"]
[null,null,"Soggetti",null,"Manned space flight"]
[{"$nulls":4},"Astronautics and state - United States"]
[{"$nulls":4},"Moon Exploration"]
[{"$nulls":4},"Mars (Planet) Exploration"]
[null,null,"Lingua di pubblicazione",null,"Inglese"]
[null,null,"Formato",null,"Materiale a stampa"]
[null,null,null,"Livello bibliografico","Monografia"]
[null,null,null,"Nota di bibliografia","Includes bibliographical references and index."]
[null,null,"Nota di contenuto",null,"Chapter 1: introduction and the importance of Focusing on the Moon -- Chapter 2: Understanding the Options for Human Exploration and Ultimate Colonization -- Chapter 3: The Advantages of the Moon in terms of Transportation, Fuel Expenditures, Access to Water, and Expense -- Chapter 4: The Advantages of the Moon in terms of Telecommunications and IT Services -- Chapter 5: The Advantages of the Moon in the Context of Near Instantaneous Support Services from Earth -- Chapter 6: Financial, Technical, Utility Infrastructure feasibility to creating a Lunar Colony/ Habitat versus doing so on Mars -- Chapter 7: Examining New Capabilities that could be Created and Tested on the Moon before seeking a Permanent Mars Presence -- Chapter 8: Planning for the Future -- Chapter 9: Conclusions and Next Steps -- Appendix 1: Key Terms and Concepts -- Appendix 2: Space Policy Directive 1 -- About the Author -- Index."]
[null,null,null,"Sommario/riassunto","This SpringerBrief explores the technological, economic, physiological, and psychological comparisons between a journey to the Moon versus a journey to Mars, taking into consideration the national and international perspectives at play. The author spent over six years interviewing leading space experts from around the world to learn why lunar habitats and the creation of a permanent presence on the Moon are an essential next step to human exploration and settlement in space. Practical reasons related to energy, telecommunications and "]
[{"$nulls":4},"networking, robotic systems, medical and scientific research, material processing, and more show why it must be the Moon First and Mars Second. These findings and recommendations have been adopted by current NASA Administrator Jim Bridenstine, as well as the current U. S. president. The research in this text reflects the author's experiences working internally within NASA Headquarters, the FAA Commercial Spaceflight Office, as well as the International Space University. It is partially based on Reneau’s award-winning Harvard thesis in conjunction with her Master's in International Relations."]
[null,"2.","Record Nr.",null,"UNINA9910830959103321"]
[null,null,"Titolo",null,"Genetic manipulation : techniques and applications / / edited by J.M. Grange, A. Fox & N.L. Morgan"]
[null,null,"Pubbl/distr/stampa",null,"Oxford ; ; Boston, : Blackwell Scientific Publications, 1991"]
[null,null,"ISBN",null,"1-282-23726-8"]
[{"$nulls":4},"9786612237263"]
[{"$nulls":4},"1-4443-1414-9"]
[{"$nulls":4},"1-4443-1413-0"]
[null,null,"Descrizione fisica",null,"1 online resource (417 p.)"]
[null,null,"Collana",null,"The Society for Applied Bacteriology technical series ; ; no. 28"]
[null,null,"Disciplina",null,"575.10724"]
[{"$nulls":4},"660/.65"]
[null,null,"Soggetti",null,"Genetic engineering"]
[{"$nulls":4},"Biotechnology"]
[null,null,"Lingua di pubblicazione",null,"Inglese"]
[null,null,"Formato",null,"Materiale a stampa"]
[null,null,null,"Livello bibliografico","Monografia"]
[null,null,null,"Note generali","\"Based on the Society of Applied Bacteriology Autumn Meeting held at the South Bank Polytechnic, London, on 18 October 1989\"--Pref."]
[null,null,null,"Nota di bibliografia","Includes bibliographical references and index."]
[null,null,"Nota di contenuto",null,"Genetic Manipulation: Techniques and Applications; Contents; Contributors; Preface; Extraction, Purification and Assay of DNA; Isolation of DNA; Isolation of Plasmid DNA from Bacteria; Purification of DNA; Isolating Genomic DNA From Bacterial Cells; Isolating Mammalian DNA; Assay of DNA; References; Restriction Mapping of DNA; Restriction Enzymes; Approaches to Mapping; References; The Construction and Use of Cloning Vectors; The Commercially Available Vectors; The Construction of Vectors for Use with the Clostridia; References; Appendix I: Addresses of Suppliers of Cloning Vectors"]
[{"$nulls":4},"Appendix II: Addresses of Suppliers of Other Products Useful for Molecular BiologyAdaptor Based cDNA Cloning in the Phage Vectors lgt10 and lgt11; Experimental Methods; Results of cDNA Library Analysis; Discussion; Acknowledgements; References; Plasmid Profiling and DNA/DNA Hybridization for Distinguishing Between Mesophilic Aeromonas Bacteria; DNA/DNA Hybridization; Plasmid Profiling; Methods; Results; Acknowledgements; References; Appendix: Reagents; Preparation and Screening of Bacterial Genomic Libraries; General Strategy; Construction of a Genomic Library by using lEMBL4"]
[{"$nulls":4},"Screening of a lEMBL4 Library with Gene ProbesConstruction of a lgt11 Library and Screening with Antibodies; Acknowledgements; References; "]
[{"$nulls":4},"DNA Probes for Detection and Identification of Bacteria; Principle of Nucleic Acid Hybridization; Application of DNA Hybridization; The Polymerase Chain Reaction; References; Extraction and Purification of Eukaryotic mRNA; Basic Precautions Prior to RNA Extraction; RNA Extraction Protocols; Analysis of Purified RNA; Isolation of Polyadenylated mRNA from Total RNA; Storage of RNA Samples; Acknowledgements; References; cDNA Cloning"]
[{"$nulls":4},"How Many Clones are Required?Cloning Strategies; First-strand Synthesis; Second-strand Synthesis; Preparation of the cDNA for Cloning; Methylation of the cDNA; Linker Kinase Reaction; Test of Ligation Efficiency; Ligation of the Phosphorylated Linkers to the cDNA; EcoRI Digestion and Removal of Excess Linkers from the cDNA; Size Fractionation of the Linkered cDNA; Ligating Fragments into pUC Vectors; Transformation of Host Bacteria; Screening for Recombinant Phages and Plasmids; References; Primer Extension Sequencing of RNA Viruses; Precautions when Handling RNA"]
[{"$nulls":4},"Nucleotide Sequencing of Influenza Virus GenesPrimer Extension Sequencing of Influenza A Viruses; Primer Extension Sequencing of Flavivirus Genes; Comments; References; Appendix: Buffers and Reagents; Forensic Applications of DNA Profiling; MuItiIocus Probes; Single Locus Probes; Methods; DNA Profiling in Forensic Casework; References; Application of Nucleic Acid Probes to the Identification of Bacterial Enteric Pathogens; Identification of Enterotoxigenic Escherichia coli from Faecal Specimens; Gene Probes for other Bacterial Enteric Pathogens"]
[{"$nulls":4},"Prospects for Nucleic Acid Probes in Clinical Microbiology"]
[null,null,null,"Sommario/riassunto","Genetic manipulation is no longer the province of the specialized researcher. It is finding widespread application in all fields of medicine and biology. Nevertheless, application of these relatively new techniques to new areas of research is often fraught with unexpected problems and difficulties. Based on the Society for Applied Bacteriology's Autumn 1989 Conference, this unique volume covers a wide and very up-to-date range of techniques used in genetic engineering. These include the isolation and analysis of DNA and RNA from cells and tissues, the selection and use of phage and plasmic vec"]