{"text_format":"2","workbook":"xls","n_sheets":1,"sheets":["report2"],"not_rendered":{"charts":0,"images":0,"macros":0,"external_links":1,"defined_names":2,"comments":0,"pivot_tables":0},"unopened":[],"bounds":{"document_bytes":16777216,"sheet_cells":100000,"run_cost":"one","rows_first":2000,"rows_last":500,"rows_between":2000,"halvings":0,"cell_chars":16384,"cell_head_chars":4096},"cells_cut":0,"cells_raw":0,"read":"whole","date_system":"1900","formulas":"cached_only"}

{"sheet":"report2","index":0,"n_rows":48,"n_cols":5,"rows_kept":48,"cut":false,"hidden":false,"merged":[[0,2,0,3],[0,4,0,5],[1,2,1,3],[1,4,1,5],[2,2,2,3],[2,4,2,5],[3,2,3,3],[4,2,4,3],[7,2,7,3],[8,2,8,3],[9,2,9,3],[11,2,11,3],[14,2,14,3],[15,2,15,3],[19,2,19,3],[26,2,26,3],[26,4,26,5],[27,2,27,3],[27,4,27,5],[28,2,28,3],[28,4,28,5],[29,2,29,3],[32,2,32,3],[33,2,33,3],[34,2,34,3],[35,2,35,3],[40,2,40,3],[41,2,41,3],[43,2,43,3]],"flags":["formulas_cached_only"]}
[null,"1.","Record Nr.",null,"UNINA9910457970603321"]
[null,null,"Autore",null,"Becker Jeffrey M"]
[null,null,"Titolo",null,"Biotechnology [[electronic resource] ] : a laboratory course / / Jeffrey M. Becker, Guy A. Caldwell, Eve Ann Zachgo"]
[null,null,"Pubbl/distr/stampa",null,"San Diego, : Academic Press, c1996"]
[null,null,"ISBN",null,"1-281-18650-3"]
[{"$nulls":4},"9786611186500"]
[{"$nulls":4},"0-08-052819-8"]
[null,null,"Edizione",null,"[2nd ed.]"]
[null,null,"Descrizione fisica",null,"1 online resource (283 p.)"]
[null,null,"Disciplina",null,"660.6"]
[{"$nulls":4},"660/.6/078 20"]
[null,null,"Soggetti",null,"Biochemical engineering"]
[{"$nulls":4},"Biotechnology"]
[{"$nulls":4},"Electronic books."]
[null,null,"Lingua di pubblicazione",null,"Inglese"]
[null,null,"Formato",null,"Materiale a stampa"]
[null,null,null,"Livello bibliografico","Monografia"]
[null,null,null,"Note generali","Description based upon print version of record."]
[null,null,null,"Nota di bibliografia","Includes bibliographical references and index."]
[null,null,"Nota di contenuto",null,"Front Cover; Biotechnology: A Laboratory Course; Copyright Page; Contents; Preface to the Second Edition; Preface to the First Edition; Acknowledgments; Suggested Schedule for Exercises; Introductory Notes: Record Keeping and Safety Rules; Format of Student Laboratory Records; The Ten Commandments of Record Keeping; Safety Rules in the Laboratory; Exercise 1. Aseptic Technique and Establishing Pure Cultures: The Streak Plate and Culture Transfer; Exercise 2. Preparation of Culture Media; Exercise 3. The Growth Curve; Exercise 4. Isolation of Plasmid DNA from Escherichia coli: The Mini-Prep"]
[{"$nulls":4},"Exercise 5. Purification, Concentration, and Quantitation of DNAExercise 6. Large-Scale Isolation of Plasmid DNA by Column Chromatography; Exercise 7. Amplification of a lacZ Gene Fragment by the Polymerase Chain Reaction; Exercise 8. Restriction Digestion and Agarose Gel Electrophoresis; Exercise 9. Southern Transfer; Exercise 10. Preparation, Purification, and Hybridization of Probe; Exercise 11. Transformation of Saccharomyces cerevisiae; Exercise 12. Isolation of Plasmid from Yeast and Escherichia coil Transformation; Exercise 13. Protein Assays"]
[{"$nulls":4},"Exercise 14. Qualitative Assay for b-Galactosidase in Yeast ColoniesExercise 15. Determination of b-Galactosidase in Permeabilized Yeast Cells; Exercise 16. Assay of b-Galactosidase in Cell "]
[{"$nulls":4},"Extracts; Exercise 17. b-Galactosidase Purification; Exercise 18. Western Blot: Probe of Protein Blot with Antibody to b-Galactosidase; Appendix 1. Alternative Protocols and Experiments; Exercise 1A Isolation and Characterization of Auxotrophic Yeast Mutants; Exercise 2A Measurement of pH; Exercise 3A Use of the Spectrophotometer; Exercise 6A Isolation of Plasmid DNA: The Maxi-Prep"]
[{"$nulls":4},"Exercise 10A Colony HybridizationAppendix 2. Buffer Solutions; Appendix 3. Preparation of Buffers and Solutions; Appendix 4. Properties of Some Common Concentrated Acids and Bases; Appendix 5. Use of Micropipettors; Appendix 6. Safe Handling of Microorganisms; Appendix 7. List of Cultures; Appendix 8. Storage of Cultures and DNA; Appendix 9. Sterilization Methods; Appendix 10. Preparation of Stock Solutions for Culture Media; Appendix 11. Growth in Liquid Medium; Appendix 12. Determination of Viable Cells; Appendix 13. Determination of Cell Mass; Appendix 14. Determination of Cell Number"]
[{"$nulls":4},"Appendix 15. Nomenclature of StrainsAppendix 16. Glassware and Plasticware; Appendix 17. Preparation of Tris and EDTA; Appendix 18. Basic Rules for Handling Enzymes; Appendix 19. Effects of Common Contaminants on Protein Assays; Appendix 20. Manufacturers' and Distributors' Addresses; Appendix 21. Surfing the Bionet: World Wide Web Addresses; Glossary; Index"]
[null,null,null,"Sommario/riassunto","The objectives of this Second Edition of Biotechnology: A Laboratory Course remain unchanged: to create a text that consists of a series of laboratory exercises that integrate molecular biology with protein biochemistry techniques while providing a continuum of experiments. The course begins with basic techniques and culminates in the utilization of previously acquired technical experience and experimental material. Two organisms, Sacchaomyces cerevisiae and Escherichia coli, a single plasmid, and a single enzyme are the experimental material, yet the procedures and"]
[null,"2.","Record Nr.",null,"UNINA9910513706203321"]
[null,null,"Autore",null,"Grant M. J"]
[null,null,"Titolo",null,"Auld Lang Syne : A Song and Its Culture"]
[null,null,"Pubbl/distr/stampa",null,"Open Book Publishers, 2021"]
[{"$nulls":4},"Cambridge : , : Open Book Publishers, , 2021"]
[{"$nulls":4},"©2021"]
[null,null,"ISBN",null,"1-80064-067-6"]
[null,null,"Edizione",null,"[1st ed.]"]
[null,null,"Descrizione fisica",null,"1 online resource (360 pages)"]
[null,null,"Soggetti",null,"Theory of music & musicology"]
[{"$nulls":4},"Music reviews & criticism"]
[{"$nulls":4},"Folk & traditional music"]
[{"$nulls":4},"Scotland"]
[{"$nulls":4},"Social & cultural history"]
[null,null,"Lingua di pubblicazione",null,"Inglese"]
[null,null,"Formato",null,"Materiale a stampa"]
[null,null,null,"Livello bibliografico","Monografia"]
[null,null,"Nota di contenuto",null,"Intro -- Contents -- Introduction -- Note on the Text -- Acknowledgements -- 1. Elements of a Theory of Song -- 1.1 The Social Functions of Song -- 1.2 The Songs Folk Sing: Some Historical Evidence -- 1.3 Implied and Inherited Significance -- 1.4 Auld Lang Syne as an Object of Research: Some Issues -- The Tunes -- The Words -- The Traditions -- 2. Auld Lang Syne: Context and Genesis -- 2.1 Being a Short Discourse on Song in the Eighteenth Century -- 2.2 Auld Lang Syne before Burns -- FIRST PART -- SECOND PART -- 2.3 The Jacobite Songs -- 3. Burns's Song -- 3.1 Mrs Dunlop's Song -- 3.2 Burns's Text -- 3.3 Burns's Tune -- 3.4 What Thomson Did -- 3.5 From M1 to M2 -- 3.6 The Legacy of the Old Songs and Two Contemporaries of the New -- 4. Auld Lang Syne in the Early Nineteenth Century -- 4.1 \"We'll toom the cup to friendship's growth\" -- 4.2 The Establishment of M2 -- 4.3 Performance and Periodicals -- 4.4 Mr Sinclair's Song -- 4.5 After Rob Roy Macgregor -- 4.6 American Sources -- 5. The Song of Union -- 5.1 The Freemasons -- 5.2 The Fraternalist's Song -- 5.3 Immortal Memory: The Burns Clubs and the Burns Cult -- 5.4 Solidarity -- 6. The Song of Parting -- 6.1 Good Night, And Joy Be With You All -- 6.2 The Song of Empire -- 6.3 The Song of Parting -- 7. The Folk's Song -- 7.1 Mr Micawber's Song -- 7.2 The Song of Conflict and Reconciliation -- 7.3 Variations on a Theme -- 7.4 Iconography and "]
[{"$nulls":4},"Reminiscence -- 7.5 The Sentimentalist's Song -- 7.6 Auld Lang Syne at the Threshold of the Information Revolution -- 8.The Song of New Year -- 8.1 A Guid New Year To Ane And A': The Scots and New Year -- 8.2 New Year at St. Paul's -- 8.3 America and the Bells -- 8.4 Traditions Come Together -- 9. Take Leave, Brothers: The German Reception of Auld Lang Syne -- 9.1 The Art Composer's Song -- 9.2 Active and Passive Reception -- 9.3 The Scout's Song."]
[{"$nulls":4},"9.4 Closing the Circle -- 10. A Song Abroad -- 10.1 Princess Constance Magogo's Song -- 10.2 Foreign-Language Versions of Auld Lang Syne -- 10.3 Bells and Anthems -- 10.4 Quotation and Quodlibet -- 10.5 The Song of War and Peace -- 10.6 Threads Lead Back to the Centre -- 11. Preliminary Conclusions: A Song and Its Culture -- 12. Auld Acquaintance: Auld Lang Syne Comes Home -- 12.1 The Road to Devolution -- 12.2 The Return of M1 and the Rise of M3 -- 12.3 What Does Auld Lang Syne Have to Do with Burns? -- Appendix 1: Eight Jacobite Songs Related to Auld Lang Syne -- 1. \"The true Scots Mens Lament for the Loss of the Rights of their Ancient Kingdom\", published by John Read of Pearson's Close Edinburgh, 1718. -- 2. \"A SONG To the tune of AULD LANG SYNE\" -- 3. \"A ballad for those whose honour is sound, Who cannot be named, and must not be found. Written by a Sculpter in the Year 1746\" -- 4. Jacobite \"Auld Lang Syne\" attributed to Lochiel's Regiment (Le Régiment d'Albanie), 1747 -- 5. \"Ballad. Tune Auld Lang Syne\" -- 6.  \"Song. To the same Tune\" [i.e., Auld Lang Syne] -- 7. \"Shall Monarchy Be Quite Forgot\" -- 8. Jacobite \"Auld Lang Syne\", by Andrew Lang (1844-1912) -- Appendix 2: Burns's Auld Lang Syne-The Five Versions (B1-B5) -- B1 The version sent to Frances Dunlop, 7 December 1788 -- B2 The version published in The Scots Musical Museum, 1796 -- B3 A version written by Burns into a copy of vol. I of the Scots Musical Museum -- B4 The version sent to George Thomson, September 1793 -- B5 What may have been a \"working version\", now held in the Burns Cottage Museum in Alloway -- Appendix 3: Seven Parodies and Contrafacta from The Universal Songster, vols. II-III (1829, 1834) -- 1. \"I'll drive dull sorrow from my mind\" -- 2. \"'Tis true this life's a languid stream\" -- 3. \"Winny won't be mine\" -- 4. \"Should brandy ever be forgot? A parody\"."]
[{"$nulls":4},"5. \"Auld lang syne\" (J. H. Dixon) -- 6. \"Should lovers' joys be e'er forgot?\" -- 7. \"War was proclaimed 'twixt love and I\" -- Appendix 4: Eight Nineteenth-Century German Translations -- 1. \"Die alte gute Zeit\" (Wilhelm Gerhard) -- 2. \"Soll alte Freundschaft vergessen sein\" (Eduard Fiedler) -- 3. \"Die alte Zeit\" (Heinrich Julius Heintze) -- 4. \"'S ist lange her\" (L. G. Silbergleit) -- 5. \"Die liebe, alte Zeit\" (Otto Baisch) -- 6. \"Lang, lang dohin\" (Gustav Legerlotz) -- 7. \"Die gute alte Zeit\" (Wilhelmine Prinzhorn) -- 8. Auf gute alte Zeit (K. Bartsch) -- Appendix 5: Four Versions in Jèrriais -- 1. Version by Ph'lippe Langliais (died 1884) -- 2. Version by John D. Hubert (1895) -- 3. Version published in Nouvelle Chronique de Jersey, 15 November 1902 -- 4. Version by Mathilde dé Faye, \"Georgie\" -- Bibliography -- Bibliography I: Main Burns Editions Cited -- Bibliography II: Musical and Poetical Sources without Author/Editor Names -- Bibliography III: Other Sources Referenced Using the Author-Date System -- Discography for Recordings Discussed in Chapter 12 -- List of Illustrations -- Audio Examples -- Index."]
[null,null,null,"Sommario/riassunto","In Auld Lang Syne: A Song and its Culture, M. J. Grant explores the history of this iconic song, demonstrating how its association with ideas of fellowship, friendship and sociality has enabled it to become so significant for such a wide range of individuals and communities around the world."]