{"text_format":"2","workbook":"xls","n_sheets":1,"sheets":["report2"],"not_rendered":{"charts":0,"images":0,"macros":0,"external_links":1,"defined_names":1,"comments":0,"pivot_tables":0},"unopened":[],"bounds":{"document_bytes":16777216,"sheet_cells":100000,"run_cost":"one","rows_first":2000,"rows_last":500,"rows_between":2000,"halvings":0,"cell_chars":16384,"cell_head_chars":4096},"cells_cut":0,"cells_raw":0,"read":"whole","date_system":"1900","formulas":"cached_only"}

{"sheet":"report2","index":0,"n_rows":26,"n_cols":5,"rows_kept":26,"cut":false,"hidden":false,"merged":[[0,2,0,3],[0,4,0,5],[1,2,1,3],[1,4,1,5],[2,2,2,3],[3,2,3,3],[7,2,7,3],[8,2,8,3],[10,2,10,3],[13,2,13,3],[14,2,14,3],[18,2,18,3]],"flags":["formulas_cached_only"]}
[null,"1.","Record Nr.",null,"UNINA9910146370303321"]
[null,null,"Titolo",null,"High content screening [[electronic resource] ] : science, techniques and applications / / edited by Steven A. Haney"]
[null,null,"Pubbl/distr/stampa",null,"Hoboken, N.J., : Wiley-Interscience, c2008"]
[null,null,"ISBN",null,"1-281-28456-4"]
[{"$nulls":4},"9786611284565"]
[{"$nulls":4},"0-470-22986-1"]
[{"$nulls":4},"0-470-22985-3"]
[null,null,"Descrizione fisica",null,"1 online resource (444 p.)"]
[null,null,"Disciplina",null,"615.19"]
[{"$nulls":4},"615/.19"]
[null,null,"Soggetti",null,"Biological systems - Research - Methodology"]
[{"$nulls":4},"Combinatorial chemistry"]
[{"$nulls":4},"Computational biology"]
[null,null,"Lingua di pubblicazione",null,"Inglese"]
[null,null,"Formato",null,"Materiale a stampa"]
[null,null,null,"Livello bibliografico","Monografia"]
[null,null,null,"Note generali","Description based upon print version of record."]
[null,null,null,"Nota di bibliografia","Includes bibliographical references and index."]
[null,null,"Nota di contenuto",null,"HIGH CONTENT SCREENING; CONTENTS; Preface; Contributors; SECTION I ESSENTIALS OF HIGH CONTENT SCREENING; 1. Approaching High Content Screening and Analysis: Practical Advice for Users; 1.1 Introduction; 1.2 What is HCS and Why Should I Care?; 1.3 How does HCS Compare with Current Assay Methods?; 1.4 The Basic Requirements to Implement HCS; 1.4.1 Cell Banking; 1.4.2 Plating, Cell Density, and the Assay Environment; 1.4.3 Compound Addition and Incubation; 1.4.4 Post-Assay Processing; 1.4.5 HCS Imaging Hardware; 1.4.6 HCS Analysis Software; 1.4.7 Informatics; 1.5 The Process"]
[{"$nulls":4},"1.6 An Example Approach 1.7 Six Considerations for HCS Assays; 1.7.1 Garbage In, Garbage Out (GIGO); 1.7.2 This Is Not a Plate Reader; 1.7.3 Understand Your Biology; 1.7.4 Subtle Changes Can Be Measured and Are Significant; 1.7.5 HCS Workflow - Flexibility is the Key; 1.7.6 HCS is Hard - How Do I Learn It and Become Proficient at It?; References; 2. Automated High Content Screening Microscopy; 2.1 Introduction; 2.2 Automated HCS Imaging Requirements; 2.3 Components of Automated Imaging Platforms; 2.3.1 Fluorescence Imaging and Multiplexing; 2.3.2 Light Sources"]
[{"$nulls":4},"2.3.3 Optical Designs: Confocal Versus Wide-Field 2.3.4 Objectives; 2.3.5 Detectors; 2.3.6 Autofocus; 2.3.7 Environmental Controls and "]
[{"$nulls":4},"On-Board Liquid Handling Capabilities; 2.4 Imaging Platform Software; 2.5 Data Storage and Management; 2.6 Selecting an HCS Platform; 2.7 Comparison of a SAPK Activation HCS Assay Read on an ArrayScan(®) 3.1, an ArrayScan(®) V(Ti), and an IN Cell 3000 Automated Imaging Platform; References; 3. A Primer on Image Informatics of High Content Screening; 3.1 Background; 3.2 HCS Image Processing; 3.2.1 Image Pre-Processing"]
[{"$nulls":4},"3.2.2 Cell Detection, Segmentation, and Centerline Extraction 3.2.2.1 Cell Detection; 3.2.2.2 Particle Detection; 3.2.2.3 Cell Segmentation; 3.2.2.4 Centerline/Neurite Extraction; 3.2.3 Cell Tracking and Registration; 3.2.3.1 Simple Matching Algorithm; 3.2.3.2 Mean Shift; 3.2.3.3 Kalman Filter; 3.2.3.4 Mutual Information; 3.2.3.5 Fuzzy-System-Based Tracking; 3.2.3.6 Parallel Tracking; 3.2.4 Feature Extraction; 3.2.4.1 Features Extracted from Markov Chain Modeling of Time-Lapse Images; 3.3 Validation; 3.4 Information System Management; 3.5 Data Modeling"]
[{"$nulls":4},"3.5.1 Novel Phenotype Discovery Using Clustering 3.5.2 Gene Function Study Using Clustering; 3.5.3 Screening Hits Selection and Gene Scoring for Effectors Discovery; 3.5.3.1 Fuzzy Gene Score Regression Model; 3.5.3.2 Experimental Results; 3.5.4 Metabolic Networks Validated by Using Genomics, Proteomics, and HCS; 3.5.5 Connecting HCS Analysis and Systems Biology; 3.5.6 Metabolic Networks; 3.6 Conclusions; 3.7 Acknowledgments; References; 4. Developing Robust High Content Assays; 4.1 Introduction; 4.2 Overview of a Typical Immunofluorescence-Based High Content Assay; 4.2.1 Staining Protocol"]
[{"$nulls":4},"4.2.2 Sources of Variability"]
[null,null,null,"Sommario/riassunto","The authoritative reference on High Content Screening (HCS) in biological and pharmaceutical research, this guide covers: the basics of HCS: examples of HCS used in biological applications and early drug discovery, emphasizing oncology and neuroscience; the use of HCS across the drug development pipeline; and data management, data analysis, and systems biology, with guidelines for using large datasets. With an accompanying CD-ROM, this is the premier reference on HCS for researchers, lab managers, and graduate students."]