LEADER 03417oam 2200805zu 450 001 9910146244503321 005 20210721053955.0 010 $a1-280-55837-7 010 $a9786610558377 010 $a3-527-60064-7 035 $a(CKB)1000000000019305 035 $a(SSID)ssj0000294044 035 $a(PQKBManifestationID)11222947 035 $a(PQKBTitleCode)TC0000294044 035 $a(PQKBWorkID)10303276 035 $a(PQKB)10668017 035 $a(MiAaPQ)EBC4956603 035 $a(Au-PeEL)EBL4956603 035 $a(CaONFJC)MIL55837 035 $a(OCoLC)1024267738 035 $a(EXLCZ)991000000000019305 100 $a20160829d2002 uy 101 0 $aeng 135 $aurcnu|||||||| 181 $ctxt 182 $cc 183 $acr 200 00$aDirected molecular evolution of proteins : or how to improve enzymes for biocatalysis 210 31$a[Place of publication not identified]$cWiley VCH$d2002 215 $a1 online resource (359 pages) 300 $aBibliographic Level Mode of Issuance: Monograph 311 $a3-527-30423-1 330 $aNatural selection created optimal catalysts. However, optimal performance of enzyme catalysis does not necessarily refer to maximum reaction rate. Rather, it may be a compromise between specificity, rate, stability, and other chemical constraints that makes enzymes capable of catalyzing reactions under mild conditions and with high substrate specificity, accompanied by high regio- and enantioselectivity. The book presented here focuses on the directed evolution of proteins, which has established itself as a powerful method for designing enzymes showing new substrate specificities. It includes a comprehensive repertoire of techniques for producing combinatorial enzyme libraries, while the functional gene expression in a suitable host helps in selecting the appropriate structure, making fast screening a necessity. 606 $aEnzymes$xBiotechnology 606 $aBiocatalysis$xEvolution 606 $aProteins$xChemical modification 606 $aProteins 606 $aMolecular evolution 606 $aCombinatorial chemistry 606 $aDirected Molecular Evolution 606 $aProtein Engineering 606 $aGenetic Engineering 606 $aGenetic Techniques 606 $aInvestigative Techniques 606 $aAnalytical, Diagnostic and Therapeutic Techniques and Equipment 606 $aBiomedical Engineering$2HILCC 606 $aHealth & Biological Sciences$2HILCC 608 $aElectronic books. 615 0$aEnzymes$xBiotechnology 615 0$aBiocatalysis$xEvolution 615 0$aProteins$xChemical modification 615 0$aProteins 615 0$aMolecular evolution 615 0$aCombinatorial chemistry 615 2$aDirected Molecular Evolution 615 2$aProtein Engineering 615 2$aGenetic Engineering 615 2$aGenetic Techniques 615 2$aInvestigative Techniques 615 2$aAnalytical, Diagnostic and Therapeutic Techniques and Equipment 615 7$aBiomedical Engineering 615 7$aHealth & Biological Sciences 676 $a660.6/34 702 $aBrakmann$b Susanne 702 $aJohnsson$b Kai 801 0$bPQKB 906 $aBOOK 912 $a9910146244503321 996 $aDirected molecular evolution of proteins : or how to improve enzymes for biocatalysis$92154822 997 $aUNINA LEADER 01187nam a22002651i 4500 001 991000135729707536 005 20020913132757.0 008 020913s1986 it |||||||||||||||||ita 035 $ab11964418-39ule_inst 035 $aARCHE-005421$9ExL 040 $aDip.to Filologia Ling. e Lett.$bita$cA.t.i. Arché s.c.r.l. Pandora Sicilia s.r.l. 100 1 $aD'Addio Colosimo, Wanda$0195554 245 10$aStrategie di apprendimento e strategie comunicative :$brisultati di una ricerca nella scuola media /$cWanda d'Addio, Serena Ambroso e Paola Giunchi 260 $aRoma :$bIl Bagatto,$cc1986 300 $a144 p. ;$c25 cm 490 0$aLinguaggio alla sapienza.$pSerie ricerche ;$v1 650 4$aLingue$xInsegnamento 700 1 $aAmbroso, Serena$eauthor$4http://id.loc.gov/vocabulary/relators/aut$0224035 700 1 $aGiunchi, Paola 907 $a.b11964418$b28-04-17$c01-04-03 912 $a991000135729707536 945 $aLE008 Ist. Glott. X I 32$g1$i2008000469856$lle008$o-$pE0.00$q-$rl$s- $t0$u1$v0$w1$x0$y.i12242421$z01-04-03 996 $aStrategie di apprendimento e strategie comunicative$91457458 997 $aUNISALENTO 998 $ale008$b01-04-03$cm$da $e-$fita$git $h0$i1