LEADER 04184nam 2200649 450 001 996308751403316 005 20221206172600.0 010 $a1-5231-0070-2 010 $a3-11-042625-0 010 $a3-11-041133-4 024 7 $a10.2478/9783110411331 035 $a(CKB)3710000000346226 035 $a(SSID)ssj0001535743 035 $a(PQKBManifestationID)11835763 035 $a(PQKBTitleCode)TC0001535743 035 $a(PQKBWorkID)11502844 035 $a(PQKB)11276661 035 $a(MiAaPQ)EBC1787150 035 $a(DE-B1597)445659 035 $a(OCoLC)1013967206 035 $a(OCoLC)954878217 035 $a(DE-B1597)9783110411331 035 $a(Au-PeEL)EBL1787150 035 $a(CaPaEBR)ebr11054948 035 $a(OCoLC)939262791 035 $a(EXLCZ)993710000000346226 100 $a20150531h20142014 uy 0 101 0 $aeng 135 $aurcnu|||||||| 181 $ctxt 182 $cc 183 $acr 200 10$aBiochemistry laboratory manual for undergraduates $ean inquiry-based approach /$fTimea Gerczei, Scott Pattison; managing editor, Anna Rulka 210 1$aWarsaw, [Poland] ;$aBerlin, [Germany] :$cDe Gruyter Open,$d2014. 210 4$dİ2014 215 $a1 online resource (186 pages) $cillustrations, (some colour) 300 $aBibliographic Level Mode of Issuance: Monograph 311 $a3-11-041132-6 327 $tFront matter --$tPreface --$tContents --$t1 Introducing the Bacterial Antibiotic Sensor Mini Project --$t2 Identifying Conserved Elements in the Toxin Sensor and Designing Mutants to Test Whether They are Important for Function --$t3 Designing Primers for Site-Directed Mutagenesis --$t4 Performing Site-Directed Mutagenesis --$t5 Purifying Mutant Toxin Sensor DNA from Bacterial Cells and Evaluating its Quality Using Agarose Gel Electrophoresis and UV Spectroscopy --$t6 Preparing DNA Template for Mutant RNA Sensor Synthesis Using a Restriction Endonuclease --$t7 Synthesizing the ykkCD Mutant Toxin Sensor RNA in vitro --$t8 Purifying the ykkCD Mutant Toxin Sensor RNA and Evaluating its Purity Using Denaturing PAGE and UV spectrometry --$t9 Evaluating the Ability of the ykkCD Toxin Sensor to Recognize the Antibiotic Tetracycline Using Fluorescent Quenching --$t10 Evaluating Antibiotic Binding to Blood Serum Albumin Using Fluorescence Spectroscopy --$t11 Understanding the Importance of Buffers in Biological Systems --$t12 Molecular Visualization of an Enzyme, Acetylcholinesterase --$t13 Determining the Efficiency of the Enzyme Acetylcholine Esterase Using Steady-State Kinetic Experiment --$t14 Separation of the Phosphatidylcholines Using Reverse Phase HPLC --$tList of Figures 330 $aBiochemistry laboratory manual for undergraduates - an inquiry based approach by Gerczei and Pattison is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology while incorporating the blossoming field of bioinformatics. The novelty of this manual is the incorporation of a student-driven real real-life research project into the undergraduate curriculum. Since students test their own mutant design, even the most experienced students remain engaged with the process, while the less experienced ones get their first taste of biochemistry research. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic. 606 $aBiochemistry$vLaboratory manuals 606 $aMolecular biology$vLaboratory manuals 606 $aDrug resistance in microorganisms$vLaboratory manuals 615 0$aBiochemistry 615 0$aMolecular biology 615 0$aDrug resistance in microorganisms 676 $a572.078 700 $aGerczei$b Timea$0987175 702 $aPattison$b Scott$f1947-, 702 $aRulka$b Anna 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a996308751403316 996 $aBiochemistry laboratory manual for undergraduates$92256096 997 $aUNISA LEADER 01091nam 2200265 450 001 996686876303316 005 20251117155815.0 100 $a20251117f1982----km y0itay5003 ba 101 0 $aita 102 $aIT 105 $ay 00 y 200 1 $aAlcune considerazioni di natura economica sui progetti di riforma della legislazione cooperativa presentati al Parlamento nella 7. legislatura$fdi Ottorino Chillemi 210 $a[S.l.$cs.n.$d1982] (Vallagarina : Manfrini) 215 $a49-64 p.$d24 cm 300 $aEstratto da: Economia banca e congiuntura : rivista trimestrale della Banca di Trento e Bolzano, anno 4, n. 1, 1. trimestre (1982) 606 0 $aCooperative$xProposte di legge$2BNCF 676 $a334 700 1$aCHILLEMI,$bOttorino$0118636 801 0$aIT$bcba$gREICAT 912 $a996686876303316 951 $aXVI.7.Misc. 432$b434 FBUO$cXVI.7.Misc. 959 $aBK 969 $aFBUO 996 $aAlcune considerazioni di natura economica sui progetti di riforma della legislazione cooperativa presentati al Parlamento nella 7. legislatura$94455873 997 $aUNISA