LEADER 03053nam 2200673 450 001 9910463760803321 005 20200520144314.0 010 $a0-8157-2646-5 035 $a(CKB)2670000000568850 035 $a(EBL)3004467 035 $a(OCoLC)923616208 035 $a(MiAaPQ)EBC5179912 035 $a(MiAaPQ)EBC3004467 035 $a(Au-PeEL)EBL5179912 035 $a(CaPaEBR)ebr11528247 035 $a(OCoLC)1015880666 035 $a(EXLCZ)992670000000568850 100 $a20180206h20142014 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $2rdacontent 182 $2rdamedia 183 $2rdacarrier 200 10$aInside out, India and China $elocal politics go global /$fWilliam Antholis 210 1$aWashington, DC :$cBrookings Institution Press,$d2014. 210 4$dİ2014 215 $a1 online resource (262 p.) 225 1 $aBrookings FOCUS Book 300 $aDescription based upon print version of record. 311 $a0-8157-2641-4 311 $a1-322-15174-1 320 $aIncludes bibliographical references and index. 327 $aFront Cover; Title Page; Copyright Information; Table of Contents; Power Paradox: Seizing Control to Give It Away; Jigsaw: Counting to 1.3 Billion; Less Than Perfect Unions; China: Promised Lands, Heartlands, and Badlands; India: Forward States, Backward States, and Swing States; Power Politics: Inside Out; Differently the Same: Inside Out Diplomacy; Acknowledgments; Notes; Index; Back Cover 330 $aOne third of humanity is governed by two capitals, New Delhi and Beijing. Increasingly, these two countries are being led not from the top down, but rather from the Inside Out. In 2014, India overwhelmingly elected Narendra Modi minister, a man who rose to national prominence as chief minister of Gujarat, India's fastest growing state. Likewise, in 2013, Xi Jinping took over as president of China, having served as top official in Zhejiang and Shanghai, two of China's most prosperous provinces. Anticipating these trends and leadership transitions, William Antholis spent five months in 2012 trav 410 0$aBrookings FOCUS Book 606 $aRegionalism$xPolitical aspects$zIndia 606 $aLocal government$zIndia 606 $aFree enterprise$zIndia 606 $aRegionalism$xPolitical aspects$zChina 606 $aLocal government$zChina 606 $aFree enterprise$zChina 607 $aIndia$xEconomic conditions$xRegional disparities 607 $aChina$xEconomic conditions$xRegional disparities 608 $aElectronic books. 615 0$aRegionalism$xPolitical aspects 615 0$aLocal government 615 0$aFree enterprise 615 0$aRegionalism$xPolitical aspects 615 0$aLocal government 615 0$aFree enterprise 676 $a320.80951 700 $aAntholis$b William$0861602 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910463760803321 996 $aInside out, India and China$91922709 997 $aUNINA LEADER 04184nam 2200649 450 001 996308751403316 005 20221206172600.0 010 $a1-5231-0070-2 010 $a3-11-042625-0 010 $a3-11-041133-4 024 7 $a10.2478/9783110411331 035 $a(CKB)3710000000346226 035 $a(SSID)ssj0001535743 035 $a(PQKBManifestationID)11835763 035 $a(PQKBTitleCode)TC0001535743 035 $a(PQKBWorkID)11502844 035 $a(PQKB)11276661 035 $a(MiAaPQ)EBC1787150 035 $a(DE-B1597)445659 035 $a(OCoLC)1013967206 035 $a(OCoLC)954878217 035 $a(DE-B1597)9783110411331 035 $a(Au-PeEL)EBL1787150 035 $a(CaPaEBR)ebr11054948 035 $a(OCoLC)939262791 035 $a(EXLCZ)993710000000346226 100 $a20150531h20142014 uy 0 101 0 $aeng 135 $aurcnu|||||||| 181 $ctxt 182 $cc 183 $acr 200 10$aBiochemistry laboratory manual for undergraduates $ean inquiry-based approach /$fTimea Gerczei, Scott Pattison; managing editor, Anna Rulka 210 1$aWarsaw, [Poland] ;$aBerlin, [Germany] :$cDe Gruyter Open,$d2014. 210 4$dİ2014 215 $a1 online resource (186 pages) $cillustrations, (some colour) 300 $aBibliographic Level Mode of Issuance: Monograph 311 $a3-11-041132-6 327 $tFront matter --$tPreface --$tContents --$t1 Introducing the Bacterial Antibiotic Sensor Mini Project --$t2 Identifying Conserved Elements in the Toxin Sensor and Designing Mutants to Test Whether They are Important for Function --$t3 Designing Primers for Site-Directed Mutagenesis --$t4 Performing Site-Directed Mutagenesis --$t5 Purifying Mutant Toxin Sensor DNA from Bacterial Cells and Evaluating its Quality Using Agarose Gel Electrophoresis and UV Spectroscopy --$t6 Preparing DNA Template for Mutant RNA Sensor Synthesis Using a Restriction Endonuclease --$t7 Synthesizing the ykkCD Mutant Toxin Sensor RNA in vitro --$t8 Purifying the ykkCD Mutant Toxin Sensor RNA and Evaluating its Purity Using Denaturing PAGE and UV spectrometry --$t9 Evaluating the Ability of the ykkCD Toxin Sensor to Recognize the Antibiotic Tetracycline Using Fluorescent Quenching --$t10 Evaluating Antibiotic Binding to Blood Serum Albumin Using Fluorescence Spectroscopy --$t11 Understanding the Importance of Buffers in Biological Systems --$t12 Molecular Visualization of an Enzyme, Acetylcholinesterase --$t13 Determining the Efficiency of the Enzyme Acetylcholine Esterase Using Steady-State Kinetic Experiment --$t14 Separation of the Phosphatidylcholines Using Reverse Phase HPLC --$tList of Figures 330 $aBiochemistry laboratory manual for undergraduates - an inquiry based approach by Gerczei and Pattison is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology while incorporating the blossoming field of bioinformatics. The novelty of this manual is the incorporation of a student-driven real real-life research project into the undergraduate curriculum. Since students test their own mutant design, even the most experienced students remain engaged with the process, while the less experienced ones get their first taste of biochemistry research. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic. 606 $aBiochemistry$vLaboratory manuals 606 $aMolecular biology$vLaboratory manuals 606 $aDrug resistance in microorganisms$vLaboratory manuals 615 0$aBiochemistry 615 0$aMolecular biology 615 0$aDrug resistance in microorganisms 676 $a572.078 700 $aGerczei$b Timea$0987175 702 $aPattison$b Scott$f1947-, 702 $aRulka$b Anna 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a996308751403316 996 $aBiochemistry laboratory manual for undergraduates$92256096 997 $aUNISA