LEADER 02256nas 2200625-a 450 001 996218774003316 005 20231101153223.0 011 $a1661-8300 035 $a(OCoLC)123336230 035 $a(CKB)1000000000256990 035 $a(CONSER)--2019205606 035 $a(DE-599)ZDB2269580-1 035 $a(EXLCZ)991000000000256990 100 $a20070420a20079999 --- a 101 0 $aeng 135 $aurmn||||||||| 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aLogica universalis 210 $aBasel $cBirkhäuser$d©2007- 210 3 $a[Switzerland] $cSpringer International Publishing 300 $aTitle from title page (SpringerLink, viewed Apr 12, 2007). 300 $aRefereed/Peer-reviewed 311 $a1661-8297 330 3 $aLogica Universalis (LU) publishes peer-reviewed research papers related to universal features of logics. Topics include general tools and techniques for studying already existing logics and building new ones, the study of classes of logics, the scope of validity and the domain of application of fundamental theorems, and also philosophical and historical aspects of general concepts of logic. 517 1 $aLU 531 $aLOG. UNIVERS 531 1 $aLog. univers. 606 $aLogic, Symbolic and mathematical$vPeriodicals 606 $aLogique symbolique et mathématique$vPériodiques 606 $aLogic, Symbolic and mathematical$2fast$3(OCoLC)fst01002068 606 $aUniverselle Logik$vZeitschrift$2swd 608 $aPeriodical 608 $aperiodicals.$2aat 608 $aPeriodicals.$2fast 608 $aPeriodicals.$2lcgft 608 $aPériodiques.$2rvmgf 610 00$awiskunde 610 00$amathematics 610 00$amathematische logica 610 00$amathematical logic 610 00$alogica 610 00$alogic 610 10$aMathematics (General) 610 10$aWiskunde (algemeen) 615 0$aLogic, Symbolic and mathematical 615 6$aLogique symbolique et mathématique 615 7$aLogic, Symbolic and mathematical. 615 7$aUniverselle Logik 676 $aQA9 906 $aJOURNAL 912 $a996218774003316 996 $aLogica universalis$92265946 997 $aUNISA LEADER 02002nam0 22004093i 450 001 UBO1171890 005 20251003044420.0 010 $a8838608768 100 $a20070731d2000 ||||0itac50 ba 101 | $aita 102 $ait 181 1$6z01$ai $bxxxe 182 1$6z01$an 200 1 $aE-valuation$evalutare le imprese Internet$fFrancesco Perrini 210 $aMilano$cMcGraw-Hill Libri Italia$d2000 215 $aXVIII, 369 p.$d24 cm. 606 $aCommercio elettronico$xValutazione economica$2FIR$3UFIC093131$9I 676 $a004.67$9$v21 676 $a658.05$9GESTIONE IN GENERALE. 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(precedente collocazione: GUERRAZZI B 1735)$fB $h20091228$i20091228 977 $a 01$a BI 996 $aE-valuation$967895 997 $aUNISANNIO LEADER 05357nam 22005775 450 001 9910253889603321 005 20251113173601.0 010 $a3-319-27216-0 024 7 $a10.1007/978-3-319-27216-0 035 $a(CKB)3710000000667110 035 $a(DE-He213)978-3-319-27216-0 035 $a(MiAaPQ)EBC4526688 035 $a(PPN)194074323 035 $a(EXLCZ)993710000000667110 100 $a20160510d2016 u| 0 101 0 $aeng 135 $aurnn|008mamaa 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aAdvanced Technologies for Protein Complex Production and Characterization /$fedited by M. Cristina Vega 205 $a1st ed. 2016. 210 1$aCham :$cSpringer International Publishing :$cImprint: Springer,$d2016. 215 $a1 online resource (XIII, 382 p. 87 illus., 71 illus. in color.) 225 1 $aAdvances in Experimental Medicine and Biology,$x2214-8019 ;$v896 311 08$a3-319-27214-4 320 $aIncludes bibliographical references and index. 327 $aProtein complex production from the drug discovery standpoint -- Choose a suitable expression host: a survey of available protein production platforms -- ACEMBL Tool-Kits for High-Throughput Multigene Delivery and Expression in Prokaryotic and Eukaryotic Hosts -- Complex reconstitution and characterization by combining co-expression techniques in Escherichia coli with high-throughput -- Membrane protein production in E. coli for applications in drug discovery -- Cell-free Synthesis of Macromolecular Complexes -- A Bacillus megaterium system for the production of recombinant proteins and protein complexes -- Protein complex production in alternative prokaryotic hosts -- Production of Protein Complexes in Non-methylotrophic and Methylotrophic Yeasts -- Leishmania tarentolae for the Production of Multi-Subunit Complexes -- Alternative Eukaryotic Expression Systems for the Production of Proteins and Protein Complexes -- Fundamentals of baculovirus expression and applications -- The MultiBac Baculovirus / Insect Cell Expression Vector System for Producing Complex Protein Biologics -- Fundamentals of expression in mammalian cells -- Assembling multi-subunit complexes using mammalian expression -- Microalgae as solar-powered protein factories -- Strategies and methodologies for the co-expression of multiple proteins in plants -- Transient expression systems in plants?potentialities and constraints -- Complex reconstitution from individual protein modules -- Structural reconstruction of protein-protein complexes involved in intracellular signaling -- The use of small-angle scattering for the characterization of multi subunit complexes -- Application of nuclear magnetic resonance and hybrid methods to structure determination of complex systems. . 330 $aThis book presents advanced expression technologies for the production of protein complexes. Since complexes lie at the heart of modern biology, the expression, purification, and characterization of large amounts of high-quality protein complexes is crucial for the fields of biomedicine, biotechnology, and structural biology. From co-expression in E. coli, yeast, mammalian and insect cells to complex reconstitution from individual subunits, this book offers useful insights and guidance for successful protein expressionists. Across several sections readers will discover existing opportunities for the production of protein complexes in bacterial systems (including membrane proteins and cell-free co-expression), methylotrophic and non-methylotrophic yeasts, protozoa (Leishmania terantolae and Dictyostelium discoideum), baculovirus-infected insect cells, mammalian cells, plants and algae. Complex reconstitution from individually purified subunits or subcomplexes is discussed as a complementary strategy. A last section introduces briefly some of the biophysical and structural characterization techniques for macromolecular complexes using state-of-the-art solution scattering and nuclear magnetic resonance. This work is a guided tour over some of the most powerful and successful protein expression technologies, with a focus on co-expression and high-throughput applications. It is addressed to everyone interested in the production and characterization of macromolecular complexes, from university students who want an accessible description of the major co-expression systems to researchers in biomedicine and the life sciences seeking for an up-to-date survey of available technologies. 410 0$aAdvances in Experimental Medicine and Biology,$x2214-8019 ;$v896 606 $aProteins 606 $aProteins 606 $aBioinformatics 606 $aProtein Biochemistry 606 $aProteins 606 $aComputational and Systems Biology 615 0$aProteins. 615 0$aProteins. 615 0$aBioinformatics. 615 14$aProtein Biochemistry. 615 24$aProteins. 615 24$aComputational and Systems Biology. 676 $a660.63 702 $aVega$b M. Cristina$4edt$4http://id.loc.gov/vocabulary/relators/edt 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910253889603321 996 $aAdvanced Technologies for Protein Complex Production and Characterization$92541158 997 $aUNINA