LEADER 00854nam1 2200325 450 001 990000367650203316 035 $a0036765 035 $aUSA010036765 035 $a(ALEPH)000036765USA01 035 $a0036765 100 $a20010321d1983----km-y0itay0103----ba 101 $aeng 102 $aUS 105 $a||||||||001yy 200 1 $aComputer communications 210 $aEnglewood Cliffs$cPrentice Hall Inc.$dc1983 215 $av.$d23 cm 410 $12001 461 1$1001-------$12001 676 $a001.64404 801 0$aIT$bsalbc$gISBD 912 $a990000367650203316 951 $a001.644 04 COM$c001.64404 959 $aBK 969 $aSCI 979 $aPATTY$b90$c20010321$lUSA01$h1230 979 $c20020403$lUSA01$h1644 979 $aPATRY$b90$c20040406$lUSA01$h1625 996 $aComputer communications$9104357 997 $aUNISA LEADER 01743nam 2200373 n 450 001 996392314503316 005 20200824121830.0 035 $a(CKB)4940000000109174 035 $a(EEBO)2240902390 035 $a(UnM)99864637e 035 $a(UnM)99864637 035 $a(EXLCZ)994940000000109174 100 $a19931201d1660 uy | 101 0 $aeng 135 $aurbn||||a|bb| 200 10$aReasons shewing the necessity of reformation of the publick [brace]1. doctrine, 2. worship, [double brace] 3. rites and ceremonies, 4. church-government, and discipline, reputed to be (but indeed, not) established by law$b[electronic resource] $eHumbly offered to the serious consideration of this present Parliament. 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[1660] 215 $a[4], 63, [1] p 300 $aAttributed to Cornelius Burges. 300 $aThe words "1. doctrine, 2. worship," are enclosed in brackets and the words "3. rites .. church-government," are bracketed together on title page. 300 $aAnnotation on Thomason copy: "Aug:". 300 $aReproduction of the original in the British Library. 330 $aeebo-0018 606 $aChurch polity$vEarly works to 1800 615 0$aChurch polity 700 $aBurges$b Cornelius$f1589?-1665.$01002137 801 0$bCu-RivES 801 1$bCu-RivES 801 2$bCStRLIN 801 2$bWaOLN 906 $aBOOK 912 $a996392314503316 996 $aReasons shewing the necessity of reformation of the publick 1. doctrine, 2. worship, double brace 3. rites and ceremonies, 4. church-government, and discipline, reputed to be (but indeed, not) established by law$92402182 997 $aUNISA LEADER 05331nam 22006734a 450 001 996213069903316 005 20230829010416.0 010 $a1-280-74882-6 010 $a9786610748822 010 $a0-470-76427-9 010 $a0-470-98887-8 010 $a1-4051-7307-6 035 $a(CKB)1000000000341810 035 $a(EBL)284294 035 $a(OCoLC)437176168 035 $a(SSID)ssj0000222365 035 $a(PQKBManifestationID)11187861 035 $a(PQKBTitleCode)TC0000222365 035 $a(PQKBWorkID)10171218 035 $a(PQKB)11000358 035 $a(MiAaPQ)EBC284294 035 $a(EXLCZ)991000000000341810 100 $a20060320d2006 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aPlant proteomics$b[electronic resource] /$fedited by Christine Finnie 210 $aOxford, UK ;$aAmes, Iowa $cBlackwell Pub.$d2006 215 $a1 online resource (276 p.) 225 1 $aAnnual plant reviews ;$vv. 28 300 $aDescription based upon print version of record. 311 $a1-4051-4429-7 320 $aIncludes bibliographical references and index. 327 $aPlant Proteomics; Contents; Preface; Contributors; 1 Plant proteomics: challenges and resources; 1.1 Introduction; 1.2 Challenges; 1.2.1 Sample extraction; 1.2.1.1 Two-dimensional gel electrophoresis; 1.2.1.2 Direct MS analysis of samples; 1.2.2 Sample preparation and arraying; 1.2.2.1 Two-dimensional gel electrophoresis; 1.2.2.2 One-dimensional gel electrophoresis; 1.2.2.3 Blue-native gel electrophoresis; 1.2.2.4 Direct analysis of samples by MS; 1.2.3 Mass spectrometry (MALDI and ESI); 1.2.3.1 MALDI; 1.2.3.2 ESI; 1.2.4 Analysis depth; 1.2.5 Data analysis; 1.2.5.1 Peptide mass fingerprints 327 $a1.2.5.2 Peptide fragmentation data (MS/MS)1.2.5.3 Analysis options; 1.2.6 Quantitation; 1.2.6.1 Gel stains; 1.2.6.2 Chemical labelling of sample; 1.2.7 Modifications; 1.2.8 Data; 1.3 Resources; 1.3.1 Proteomic databases; 1.3.2 Online proteomic tools and resources; 1.4 Future; 2 Proteomic analysis of post-translational modifications by mass spectrometry; 2.1 Summary; 2.2 Introduction; 2.3 Considerations for the experimental design of PTM analysis by proteomics; 2.4 Analysis of PTMs by proteomic approaches; 2.4.1 Phosphorylation; 2.4.2 Protein glycosylation; 2.4.3 GPI-AP; 2.4.4 Farnesylation 327 $a2.4.5 N-terminally modified proteins2.5 Conclusions and perspectives; 3 Strategies for the investigation of protein-protein interactions in plants; 3.1 Summary; 3.2 Introduction; 3.3 Biochemical procedures to characterize protein-protein interactions; 3.3.1 Chromatographic purifications; 3.3.2 Sucrose gradient ultrafiltration; 3.3.3 Native gel electrophoresis; 3.3.4 Immunoprecipitations; 3.4 Genetic procedures to characterize protein-protein interactions; 3.4.1 Yeast two-hybrid system; 3.4.2 Yeast three-hybrid system; 3.4.3 Yeast one-hybrid system 327 $a3.4.4 Limitations of yeast two-hybrid systems3.4.5 Split-ubiquitin system; 3.4.6 Bimolecular fluorescence complementation (BiFC); 3.4.7 Fo?rster resonance energy transfer (FRET); 3.4.8 Tagging technologies for the purification of protein complexes; 3.5 Cytological procedures to characterize protein-protein interactions; 3.6 Outlook; 4 Proteomics of disulphide and cysteine oxidoreduction; 4.1 Introduction; 4.2 Control of cellular redox status; 4.2.1 Sequence and structural features of proteins catalysing cysteine redox modifications; 4.2.2 Catalytic mechanisms of Trxs and Grxs 327 $a4.3 Proteomics techniques for analysis of cysteine modifications4.3.1 Reagents for cysteine labelling; 4.3.2 Disulphide mapping; 4.3.3 S-glutathionylation; 4.3.4 Cysteine SOH, SO2H and SO3H; 4.3.5 Trxs and disulphide reduction; 4.3.6 S-nitrosylation; 4.4 Conclusions and perspectives; 5 Structural proteomics; 5.1 Introduction; 5.2 Project data handling: Sesame; 5.3 ORF cloning; 5.4 E. coli cell-based protein production pipeline; 5.4.1 Large-scale protein production and labeling; 5.4.2 Protein purification; 5.5 Wheat germ cell-free protein production 327 $a5.6 Mass spectrometry of purified proteins for quality assurance and analysis 330 $aThe proteome comprises all protein species resulting from gene expression in a cell, organelle, tissue or organism. By definition, proteomics aims to identify and characterise the expression pattern, cellular location, activity, regulation, post-translational modifications, molecular interactions, three dimensional structures and functions of each protein in a biological system.In plant science, the number of proteome studies is rapidly expanding after the completion of the Arabidopsis thaliana genome sequence, and proteome analyses of other important or emerging model systems and crop 410 0$aAnnual plant reviews ;$vv. 28. 606 $aPlant proteins 606 $aPlant proteomics 615 0$aPlant proteins. 615 0$aPlant proteomics. 676 $a572.62 676 $a572/.62 676 $a580.5 686 $a42.42$2bcl 701 $aFinnie$b Christine$0863228 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a996213069903316 996 $aPlant proteomics$91926951 997 $aUNISA