LEADER 01442nam 2200325Ia 450 001 996390844003316 005 20200824132035.0 035 $a(CKB)4940000000098501 035 $a(EEBO)2248519055 035 $a(OCoLC)ocm9818801e 035 $a(OCoLC)9818801 035 $a(EXLCZ)994940000000098501 100 $a19830817d1669 uy | 101 0 $aeng 135 $aurbn||||a|bb| 200 14$aThe fulfilling of the Scripture, or, An essay shewing the exact accomplishment of the Word of God in his works of providence, performed and to be performed$b[electronic resource] $efor confirming the beleevers and convincing the atheists of the present time : containing in the end a few rare histories of the works and servants of God in the Church of Scotland 210 $a[S.l. $cs.n.]$d1669 215 $a[14], 296 p 300 $aAttributed to Robert Fleming--Wing. 300 $aReproduction of original in the University of Illinois (Urbana-Champaign Campus). Library. 330 $aeebo-0167 606 $aProvidence and government of God 615 0$aProvidence and government of God. 700 $aFleming$b Robert$f1630-1694.$01003199 801 1$bUMI 801 2$bWaOLN 906 $aBOOK 912 $a996390844003316 996 $aThe fulfilling of the Scripture, or, An essay shewing the exact accomplishment of the Word of God in his works of providence, performed and to be performed$92365570 997 $aUNISA LEADER 03880nam 22007455 450 001 996207426003316 005 20240529145243.0 010 $a3-319-16259-4 024 7 $a10.1007/978-3-319-16259-1 035 $a(CKB)3710000000415186 035 $a(SSID)ssj0001500656 035 $a(PQKBManifestationID)11967966 035 $a(PQKBTitleCode)TC0001500656 035 $a(PQKBWorkID)11535914 035 $a(PQKB)10488240 035 $a(DE-He213)978-3-319-16259-1 035 $a(MiAaPQ)EBC3565663 035 $a(MiAaPQ)EBC6422689 035 $a(Au-PeEL)EBL6422689 035 $a(OCoLC)1231605307 035 $a(PPN)186026102 035 $a(EXLCZ)993710000000415186 100 $a20150508d2015 u| 0 101 0 $aeng 135 $aurnn#008mamaa 181 $ctxt 182 $cc 183 $acr 200 10$aLow-Cost Methods for Molecular Characterization of Mutant Plants $eTissue Desiccation, DNA Extraction and Mutation Discovery: Protocols /$fby Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger 205 $a1st ed. 2015. 210 1$aCham :$cSpringer International Publishing :$cImprint: Springer,$d2015. 215 $a1 online resource (X, 35 p. 9 illus., 3 illus. in color.) 300 $aBibliographic Level Mode of Issuance: Monograph 311 $a3-319-16258-6 327 $aIntroduction -- Health and Safety Considerations -- Sample Collection and Storage -- Low-Cost DNA Extraction -- PCR Amplification for Low-Cost Mutation Discovery -- Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples -- Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants -- Example Data -- Conclusions.  . 330 $aThis book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits. 606 $aPlant biotechnology 606 $aBiology?Technique 606 $aBiomaterials 606 $aNucleic acids 606 $aPlant Biotechnology 606 $aBiological Techniques 606 $aNucleic Acid 615 0$aPlant biotechnology. 615 0$aBiology?Technique. 615 0$aBiomaterials. 615 0$aNucleic acids. 615 14$aPlant Biotechnology. 615 24$aBiological Techniques. 615 24$aNucleic Acid. 676 $a631.52 676 $a660.6 700 $aTill$b Bradley J$4aut$4http://id.loc.gov/vocabulary/relators/aut$0993843 702 $aJankowicz-Cieslak$b Joanna$4aut$4http://id.loc.gov/vocabulary/relators/aut 702 $aHuynh$b Owen A$4aut$4http://id.loc.gov/vocabulary/relators/aut 702 $aBeshir$b Mayada M$4aut$4http://id.loc.gov/vocabulary/relators/aut 702 $aLaport$b Robert G$4aut$4http://id.loc.gov/vocabulary/relators/aut 702 $aHofinger$b Bernhard J$4aut$4http://id.loc.gov/vocabulary/relators/aut 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a996207426003316 996 $aLow-Cost Methods for Molecular Characterization of Mutant Plants$92275613 997 $aUNISA