LEADER 05321nam 2200661Ia 450 001 996206293403316 005 20230421045658.0 010 $a1-282-18905-0 010 $a9786612189050 010 $a1-4443-1383-5 010 $a1-4443-1382-7 035 $a(CKB)1000000000794259 035 $a(EBL)454404 035 $a(OCoLC)609844402 035 $a(SSID)ssj0000354243 035 $a(PQKBManifestationID)11251807 035 $a(PQKBTitleCode)TC0000354243 035 $a(PQKBWorkID)10302579 035 $a(PQKB)10302913 035 $a(MiAaPQ)EBC454404 035 $a(Au-PeEL)EBL454404 035 $a(CaPaEBR)ebr10310593 035 $a(CaONFJC)MIL218905 035 $a(EXLCZ)991000000000794259 100 $a19940217d1994 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aGenetic analysis$b[electronic resource] $eprinciples, scope, and objectives /$fJohn R.S. Fincham 210 $aOxford ;$aBoston $cBlackwell Scientific Publications$d1994 215 $a1 online resource (241 p.) 300 $aDescription based upon print version of record. 311 $a0-632-03659-1 320 $aIncludes bibliographical references and index. 327 $aGENETIC ANALYSIS; CONTENTS; PREFACE; INTRODUCTION: THE EXPANDING SCOPE OF GENETICS; 1: DISSECTING THE GENOME USING NATURAL GENETIC SYSTEMS; The eukaryotic system; Eukaryotes distinguished from prokaryotes; Mitosis and chromosome structure; Alternation of haploid and diploid phases in the sexual cycle; Meiosis and the rules of classical genetics; Meiosis; Single-factor genetic ratios and their explanation; Independent assortment of different allele-pairs; Testing data for fit to theoretical ratios; Linkage groups and the nature of crossovers; Map units and map distance 327 $aPlacing Linked genes in sequenceDistinguishing between independent assorment and distant linkage; Mapping centromeres using tetrads; Sex linkage; Segregation and linkage in human genetics; Assigning linkage groups to chromosomes; Genetic analysis of bacteria and bacteriophage; Three modes of gene transfer in bacteria; Time-of-entry mapping in E. coli; Analogy with sexual recombination and segregation; Stable partial diploids - F' plasmids; Bacteriophage lambda - a virus in the chromosome; Bacteriophage T4 - a model recombinational system; Eukaryotic organelle genetics 327 $aExceptions to the rule of equal results from reciprocal crossesChloroplast variants; Mitochondrial variants; Summary and perspective; Further reading; References; 2: FROM MUTATIONS TO GENES; Defining the gene by mutation and complementation; What is it that the markers mark?; The collection of mutants; Sorting of mutants into complementation groups; An interim definition of the gene; Mapping within the gene; The detection of recombination within genes; Mapping by reference to flanking markers; Recombination frequency; Deletion mapping; The determination by genes of protein structure 327 $aColinearity of the gene and its encoded protein productBiochemistry of polypeptide synthesis; Not all genes encode proteins; Conclusions; References; 3: THE GENE AS DNA SEQUENCE; Characterization of DNA fragments; Restriction endonucleases; Separating aod sizing DNA fragments; Probing for specific sequences; Making restriction-site maps; Cloning and cloning vectors; Making recombinant DNA molecules; Escherichia coli plasmid vectors; Yeast 2-um plasmid and shuttle vectors; Lambda (l) bacteriophage and cosmid vectors; Yeast artificial chromosomes (YACs) 327 $aScreening DNA libraries for functional genesGenomic libraries and cDNA libraries; Gene identification by complementation of mutants; Screening gene libraries with DNA probes; Designing gene-specific probes; Use of cDNA probes; Screening for gene expression; Probing across species or group boundaries; Positional cloning; Confirming the identity of cloned sequences; DNA sequence and open reading frames; Using the clone to disrupt the corresponding gene; Hybridization back to chromosomes; Simultaneous mutagenesis and gene tagging; The principle; Transposon tags; Conclusion; References 327 $a4: THE EVOLVING CONCEPT OF THE GENE 330 $aAuthored by a very eminent geneticist, this text gives students a thorough appreciation of the development and potential of analytical genetic techniques. Beginning with a consideration of both the classical Mendelian and the molecular biological aspects of genetic analysis, the book goes on to discuss progress in three key areas of genetics. Firstly the elucidation of the detailed structure and overall organization of the genome, secondly the way that genetic differences at the molecular level account for heritable variation in populations, and finally an explanation of how the genes control 606 $aGenetics$xTechnique 606 $aNucleotide sequence 606 $aGene mapping 615 0$aGenetics$xTechnique. 615 0$aNucleotide sequence. 615 0$aGene mapping. 676 $a574.87/322 700 $aFincham$b J. R. S$084777 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a996206293403316 996 $aGenetic analysis$9836688 997 $aUNISA