LEADER 01615nam0-2200517---450- 001 990000056700203316 005 20081218143825.0 010 $a88-453-0957-6 035 $a0005670 035 $aUSA010005670 035 $a(ALEPH)000005670USA01 035 $a0005670 100 $a20000914d1999----|||y0itay0103----ba 101 0 $aita 102 $aIT 105 $a||||||||001yy 200 1 $a<> commercio elettronico$eguida al marketing della vendita online$fGiampiero Di Carlo 210 $aMilano$cETAS$dcopyr. 1999 215 $aXI, 222 p.$d22 cm 225 2 $aGestione d'impresa. Marketing 410 0$a12001$aGestione d'impresa. Marketing 610 $aInternet Impiego nel commercio 676 $a380. 10285 700 1$aDI CARLO,$bGiampiero$0117292 801 $aIT$bSALBC$gISBD 912 $a990000056700203316 951 $a380.102 DIC 1 (IRA 15 78)$b151224 L.M.$cIRA 15$d00009796 959 $aBK 979 $c20000914$lUSA01$h1725 979 $c20000919$lUSA01$h1047 979 $c20000919$lUSA01$h1520 979 $c20001019$lUSA01$h1055 979 $c20001019$lUSA01$h1452 979 $c20001019$lUSA01$h1500 979 $c20001019$lUSA01$h1537 979 $c20001024$lUSA01$h1513 979 $c20001027$lUSA01$h1518 979 $c20001027$lUSA01$h1522 979 $c20001110$lUSA01$h1709 979 $c20001124$lUSA01$h1207 979 $c20020403$lUSA01$h1613 979 $aPATRY$b90$c20040406$lUSA01$h1605 979 $aRSIAV1$b90$c20081218$lUSA01$h1438 996 $aCommercio elettronico$9466306 997 $aUNISA bas $agiu LEADER 05649nam 2200733 a 450 001 9910780728103321 005 20230725041509.0 010 $a1-282-75824-1 010 $a9786612758249 010 $a981-4277-19-3 035 $a(CKB)2490000000001593 035 $a(EBL)1679396 035 $a(OCoLC)850858647 035 $a(SSID)ssj0000419589 035 $a(PQKBManifestationID)12130972 035 $a(PQKBTitleCode)TC0000419589 035 $a(PQKBWorkID)10382957 035 $a(PQKB)10121739 035 $a(MiAaPQ)EBC1679396 035 $a(WSP)00000567 035 $a(Au-PeEL)EBL1679396 035 $a(CaPaEBR)ebr10421998 035 $a(CaONFJC)MIL275824 035 $a(EXLCZ)992490000000001593 100 $a20100105d2010 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aHigh-performance liquid chromatography and mass spectrometry of porphyrins, chlorophylls and bilins$b[electronic resource] /$fChang Kee Lim 210 $aRiver Edge, N.J. $cWorld Scientific$d2010 215 $a1 online resource (245 p.) 225 1 $aMethods in chromatography ;$vv. 2 300 $aDescription based upon print version of record. 311 $a981-02-3068-0 320 $aIncludes bibliographical references and index. 327 $aContents; Preface; Chapter 1. Structure, Distribution, Biosynthesis and Function; 1.1. Introduction; 1.2. Nomenclature; 1.3. Biosynthesis of Porphyrins, Haem and Chlorophyll; 1.4. Biosynthesis of Bilins in Animals and Plants; 1.4.1. Degradation of haem to bile pigments; 1.4.2. Biosynthesis of bilins in plants, algae and cyanobacteria; 1.4.3. Degradation of chlorophyll in senescent higher plants; 1.5. Function of Porphyrins and Other Tetrapyrroles; References; Chapter 2. High-Performance Liquid Chromatography of Porphyrins; 2.1. Introduction; 2.2. HPLC of Porphyrin Methyl Esters 327 $a2.3. HPLC of Porphyrin Free Acids2.3.1. Stationary phases for reversed-phase HPLC of porphyrins; 2.3.2. Mobile phases for reversed-phase HPLC of porphyrins; 2.3.3. Procedure for optimisation of porphyrin separation by reversed-phase HPLC; 2.3.4. Retention behaviour of porphyrins in reversed-phase HPLC; 2.4 HPLC of Porphyrinogens; References; Chapter 3. Mass Spectrometry of Porphyrins; 3.1. Introduction; 3.2. Fast Atom Bombardment (FAB) Mass Spectrometry of Porphyrins; 3.3. Laser Desorption/Ionisation Time-of-Flight (LDI-TOF) Mass Spectrometry of Porphyrins 327 $a3.4 Electrospray Ionisation Mass Spectrometry (ESI-MS) and HPLC/ESI-MS of Porphyrins3.4.1. HPLC/ESI-MS of porphyrins; 3.4.2. ESI-MS/MS fragmentation of porphyrins; 3.4.2.1. ESI-MS/MS product ion spectrum and fragmentation pathways of uroporphyrin; 3.4.3. ESI-MS/MS fragmentation of hydroxyuroporphyrins; 3.4.3.1. ESI-MS/MS product ion spectrum and fragmentation pathways of meso-hydroxyuroporphyrin; 3.4.3.2. ESI-MS/MS product ion spectrum of hydroxyacetic acid uroporphyrin; 3.4.3.3. ESI-MS/MS product ion spectrum of ?-hydroxypropionic acid uroporphyrin 327 $a3.4.3.4. ESI-MS/MS product ion spectrum and fragmentation pathways of ?-hydroxypropionic acid uroporphyrin3.4.3.5. ESI-MS/MS product ion spectra and fragmentation pathways of cis- and transhydroxyspirolactoneurochlorins; 3.4.3.6. ESI-MS/MS product ion spectra and fragmentation pathways of cis- and transdihydroxyurochlorins; 3.4.3.7. Characteristic ESI-MS/MS product ions of hydroxyuroporphyrins; 3.4.4. ESI-MS/MS product ion spectra of ketoacid uroporphyrins; 3.4.5. ESI-MS/MS of heptacarboxylic porphyrin; 3.4.6. ESI-MS/MS of hydroxyheptacarboxylic porphyrins 327 $a3.4.7. ESI-MS/MS of ketoacid heptacarboxylic porphyrins and formyl heptacarboxylic porphyrin3.4.8. ESI-MS/MS product ion spectrum and fragmentation pattern of coproporphyrin; 3.4.9. ESI-MS/MS of hydroxymethylcoproporphyrin; 3.4.10. ESI-MS/MS of ?-ketopropionic acid coproporphyrin and formylcoproporphyrin; 3.4.11. ESI-MS/MS product ion spectrum and fragmentation pattern of protoporphyrin; 3.4.12. HPLC/ESI-MS and MS/MS of meso-tetraphenylporphyrin derivatives; References; Chapter 4. Porphyrin Profiles in Blood, Urine and Faeces by HPLC and HPLC/ESI-MS; 4.1. Introduction 327 $a4.2. Sample Collection and Preparation 330 $a The porphyrins, chlorophylls, bilins and related tetrapyrroles are vital for all living organisms. Natural and synthetic tetrapyrroles are used extensively in foods, cosmetics, biotechnology, pharmaceuticals, diagnostics and medicine. Methods for their separation and characterization therefore, have a very wide area of applications. Yet, there is a dearth of books dedicated to HPLC and HPLC/MS of tetrapyrroles. Lim addresses this problem admirably by providing practical HPLC and HPLC/MS protocols coupled with in-depth chromatographic and mass spectrometric reference data. These are invaluable 410 0$aMethods in chromatography ;$vv. 2. 606 $aPorphyrins 606 $aChlorophyll 606 $aTetrapyrroles 606 $aHigh performance liquid chromatography 606 $aMass spectrometry 615 0$aPorphyrins. 615 0$aChlorophyll. 615 0$aTetrapyrroles. 615 0$aHigh performance liquid chromatography. 615 0$aMass spectrometry. 676 $a547/.593 700 $aLim$b Chang Kee$01519850 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910780728103321 996 $aHigh-performance liquid chromatography and mass spectrometry of porphyrins, chlorophylls and bilins$93758158 997 $aUNINA