LEADER 01770ngm 2200409 a 450 001 9910701528003321 005 20120326154502.0 035 $a(CKB)5470000002419810 035 $a(OCoLC)781641618 035 $a(EXLCZ)995470000002419810 100 $a20120326d2011 ma v 101 0 $aeng 135 $aurmna|||m|||| 181 $ctdi$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 00$aGetting help for alcohol addiction$b[electronic resource] 210 1$a[Bethesda, Md.] :$c[National Institutes of Health],$d[2011?] 215 $a1 streaming video file (5 min., 17 sec.) $cdigital, SWF file, sound, color 300 $aTitle from HTML title bar (viewed Mar. 26, 2012). 300 $aAccompanied by transcript in HTML format. 330 $aCovers a range of current treatments for alcoholism, including outpatient rehabilitation, medication, and behavioral / cognitive therapy. Features comments by Mark Willenbring, M.D., former Director, Division of Treatment and Recovery Research of the National Institute on Alcohol Abuse and Alcoholism, and includes references to NIH resources. 606 $aAlcoholics$xRehabilitation$zUnited States$vPopular works 606 $aAlcoholism$xTreatment$zUnited States$vPopular works 606 $aOlder people$xAlcohol use$zUnited States$vPopular works 608 $aEducational films.$2lcgft 615 0$aAlcoholics$xRehabilitation 615 0$aAlcoholism$xTreatment 615 0$aOlder people$xAlcohol use 701 $aWillenbring$b Mark L$01406533 712 02$aNational Institutes of Health (U.S.) 801 0$bGPO 801 1$bGPO 906 $aBOOK 912 $a9910701528003321 996 $aGetting help for alcohol addiction$93532008 997 $aUNINA LEADER 01340nam 2200421 450 001 9910795219103321 005 20230809234712.0 010 $a88-921-7068-6 035 $a(CKB)4340000000209265 035 $a(MiAaPQ)EBC5107807 035 $a(Au-PeEL)EBL5107807 035 $a(CaPaEBR)ebr11456725 035 $a(OCoLC)1007925178 035 $a(EXLCZ)994340000000209265 100 $a20171114h20172017 uy 0 101 0 $aita 135 $aurcnu|||||||| 181 $2rdacontent 182 $2rdamedia 183 $2rdacarrier 200 10$aEstratto da Causa e contratto nella prospettiva storico-comparatistica $eII Congresso Internazionale ARISTEC, Palermo-Trapani, 7-10 giugno 1995 /$fCarlo Argiroffi ; a cura di Letizia Vacca 210 1$aTorino, [Italy] :$cG. Giappichelli Editore,$d2017. 210 4$d©2017 215 $a1 online resource (97 pages) 311 $a88-921-1135-3 320 $aIncludes bibliographical references. 606 $aContracts$zItaly 615 0$aContracts 676 $a346.4502 700 $aArgiroffi$b Carlo$0229895 702 $aVacca$b Letizia 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910795219103321 996 $aEstratto da Causa e contratto nella prospettiva storico-comparatistica$93713971 997 $aUNINA LEADER 08988nam 22004693 450 001 9911028670903321 005 20250906060324.0 010 $a1-394-34294-2 010 $a1-119-87847-0 035 $a(CKB)40877791000041 035 $a(MiAaPQ)EBC32288999 035 $a(Au-PeEL)EBL32288999 035 $a(OCoLC)1535406758 035 $a(EXLCZ)9940877791000041 100 $a20250906d2025 uy 0 101 0 $aeng 135 $aur||||||||||| 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aLigand-Binding Basics $eEvaluating Intermolecular Affinity, Specificity, Stoichiometry, and Cooperativity 205 $a1st ed. 210 1$aNewark :$cJohn Wiley & Sons, Incorporated,$d2025. 210 4$d©2026. 215 $a1 online resource (307 pages) 311 08$a1-119-87842-X 327 $aCover -- Title Page -- Copyright -- Contents -- About the Cover -- Introduction -- Chapter 1 The Biology of Molecules -- Why Study Intermolecular Interactions Quantitatively? -- Equilibrium and Kinetics -- Thermodynamic Definitions of Affinity and Specificity -- The Affinity/Specificity Map -- Biology Requires Optimization of Affinity and Specificity -- The Special Case of Protein?DNA Interactions -- Chapter 2 General Theory for Reversible Ligand Binding -- Definition of Ligand and Titration -- Affinity, Specificity, Stoichiometry, and Cooperativity -- Ligand?binding Theory: Relationship to Experiment -- General Theory for Reversible Ligand Binding: Rooted in Chemical Equilibrium -- General Theory for Reversible Ligand Binding: Quantitative Treatment -- The Case of 1:1 Binding -- General Theory for Reversible Ligand Binding: Conservation of Mass -- Definition of ?? -- The Basic Equation for 1:1 Binding -- The Single Most Important Thing You Can Learn in This Book -- The Example of Heme Binding to Apocytochrome C -- The Rectangular Hyperbola -- The Binding Isotherm -- Plot of ?? vs. [Hf] -- General Theory for Reversible Ligand Binding: Role of Mass Action -- Plot of [AH] vs. [Ht] with Fixed K -- Determination of Kd from Experiment -- Plot of [AH] vs. [Ht] with Fixed [At] -- Determining Molar Ratio from Experiment -- About Activity -- Chapter 3 Graphical Analysis -- Limitations of Direct Plots -- The Semi?log Plot -- Breadth of the Semi?log Plot -- Myoglobin and Hemoglobin -- Advantages of the Direct and Semi?log Plots of Binding Data -- Linear Transforms of the Basic Binding Equation -- Common Linearizations -- Requirements of the Linear Regression Model -- A Linear Model May Misrepresent the Physical Process -- Deviations from Linearity Are Hard to Detect or Interpret -- Linear Transforms Distort Data Completeness. 327 $aLinear Transforms Invite - Even Require - Extrapolation -- Linear Transforms Falsely Promise Both K and Molar Ratio from a Single Dataset -- Summary about Linear Treatments of Binding Data -- Simulation Is Just as Good as Fitting, Given Realistic Experimental Errors -- Chapter 4 Binding of Multiple Ligands -- Conservation of Mass Outside the 1:1 Case -- Redefine ?< -- 3:mo 0:stretchy& -- equals -- "true" > -- ? to Accommodate Any Molar Ratio -- Accounting for the Definition of Molecule -- Generalizing to Integer Multiples of 1:1 -- The Langmuir Equation for Any Molar Ratio with Sites of Identical Affinity and No Cooperativity -- Adair Equation for Any Number of Binding Events -- The Langmuir Equation vs. the Adair Equation -- Thermodynamic Linkage -- Two Classes of Sites with Different Affinities -- Binding Isotherms for Multiple Sites with Different Affinities -- Summary -- Chapter 5 How to Determine Kd and Molar Ratio Experimentally -- Stoichiometric Titration First -- Amounts of Materials -- Assigning Partners -- Choice of Experimental Observables -- Choosing Solution Conditions -- How Many Data Points? -- Range?Finding Stoichiometric Titration -- Visualizing Results -- Range?Finding Asymptotic Titration to Estimate Kd -- Data Analysis -- Practicalities about Experimental Error -- Statistical Approaches to Estimate the Breakpoint -- Refined Asymptotic Titration -- Designing an Experiment to Refine Kd -- Calculating Free Ligand Concentration -- Refining the Value of Molar Ratio -- Example of ArgR/DNA Binding -- Plotting the Data -- Deriving Kd from the Data -- Summary -- Chapter 6 Cooperativity -- Facilitated and Antagonized Binding -- Free Energy Definition of Cooperative Binding -- Chemical Potential Diagram for Cooperative Binding -- Cooperativity as Non?additivity -- Reciprocity of Cooperative Effects. 327 $aLimitations of Linear Transforms for Cooperative Interactions -- Microscopic View of Species Distribution -- Homotropic and Heterotropic Cooperativity -- Cooperativity Affects Specificity as Well as Affinity -- Cooperativity Is the Third Axis of the Affinity/Specificity Map -- Quantifying Homotropic Cooperativity -- Negative Homotropic Cooperativity -- A Practical Advantage of Negative Cooperativity -- Positive Cooperativity and the Ligand Concentration Interval -- Importance of Individual?site Isotherms and Species Distribution -- Species Distributions by Specialized Experimental Methods -- The Many Forms of Cooperativity -- Emergent Properties -- Connectivity and Search Entropy -- Breakdown of Additivity in Complex Systems -- Statistical Effects -- Relevance of Non-additivity for Analysis of Mutations -- Universality and Promiscuity of Cooperativity -- Proteins as Gestalt Objects -- Summary -- Chapter 7 Theoretical and Method?specific Troubleshooting -- Equilibrium and Nonequilibrium Methods -- Accessible Concentration Ranges Limit Accessible Kd Values -- Signal from Ligand or Target? -- Separation?based Methods -- Filter Binding -- Gel Retardation or EMSA -- Gel Filtration -- Hummel and Dreyer Chromatography -- Equilibrium Dialysis -- UV Absorbance -- CD Spectroscopy -- Fluorescence -- NMR -- ITC -- AUC -- SPR -- MS -- Chapter 8 Allostery -- An Historical Overview -- Facilitated Binding -- Elaboration of the MWC Model -- Relaxed Monomers and Tense Multimers -- Positive Homotropic Cooperativity Only -- Artifactual Origins of Affinity Heterogeneity -- Relaxation of Multimers by Ligand Binding -- Koshland's Sequential (Asymmetric) Model -- G3Pase Was Heterogeneous, Not Negatively Cooperative -- Many Models Fit the Hemoglobin Data -- Advantages of Negative Cooperativity for Molecular Insight -- Biology of Negative Cooperativity. 327 $aStructural Analysis Cannot Solve Allostery -- Allostery without Cooperativity -- Summary -- Chapter 9 Lessons on Affinity and Specificity from Host/Guest Chemistry -- 2D Representations of 3D Objects -- Early Hosts Were Linear and Flexible -- Design of Molecular Properties -- Very Weak Affinity and No Detectable Specificity -- Later Hosts Pre?organized in Bound Conformation -- Enormous Gains in Affinity and Specificity -- Bonds between Host and Guest Are Identical -- Lessons from the Host/Guest Chemistry -- Rational Design of Affinity and Specificity -- Affinity and Specificity Accrue in Parallel -- Cryptic Contributions Can Dominate Binding -- Chapter 10 Reconciling Structure and Thermodynamics in Molecular Interactions -- Thermodynamics of Molecular Interactions -- Structural Analysis of Bonding Does Not Predict Binding -- The Goldilocks Region of Affinity/Specificity Space -- Conformational Rearrangement upon Binding Decouples Affinity and Specificity -- A Reservoir of Adaptability -- No Simple Reconciliation of Structural and Energetic Views -- Implications for Drug Design -- Chapter 11 Applications in Modern Drug Development -- Background -- Technological Developments -- Crystal Structures -- Trapped High?energy States -- Another Example -- Computational Methods -- High?throughput Assays -- Druggability -- Irrational Drug Design -- A New Workflow -- Appendix A Ligand?binding Study Questions -- Appendix B Thought Experiments -- Appendix C Derivations -- Appendix D Simulation and Fitting -- Simulation -- Fitting -- Appendix E About the Hill Equation -- Deriving the Hill Equation -- The Hill Equation as a Limit of the Adair Equation -- On Applying the Hill Equation to Quantify Cooperativity -- Appendix F Stereo Viewing -- Bibliography -- Index -- EULA. 330 $aA concise and accessible textbook covering ligand-binding theory in chemistry, biology, and drug development In Ligand-binding Basics: Evaluating Intermolecular Affinity, Specificity, Stoichiometry, and Cooperativity , accomplished chemist Professor Jannette Carey introduces ligand binding in a thorough and practical way for those new. 606 $aLigand binding (Biochemistry)$vTextbooks 615 0$aLigand binding (Biochemistry) 676 $a572.33 700 $aCarey$b Jannette$0756279 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9911028670903321 996 $aLigand-Binding Basics$94441377 997 $aUNINA LEADER 00931nam0 22002531i 450 001 UON00055119 005 20231205102243.240 100 $a20020107d1964 |0itac50 ba 101 $aara 102 $aEG 105 $a|||| 1|||| 200 1 $aTarih al-naqd al-'arabi$fMahammad Zaglul Sallam 210 $aal-Qahira$c[s.n.]$d1964 215 $a278 p.$d26 cm 606 $aLetteratura araba$xStoria e critica$3UONC018411$2FI 620 $aEG$dIl Cairo$3UONL000377 686 $aARA VI$cPAESI ARABI - LETTERATURA$2A 700 1$aZAGLUL SALAM$bMuhammad$3UONV027560$0648714 801 $aIT$bSOL$c20251017$gRICA 899 $aSIBA - SISTEMA BIBLIOTECARIO DI ATENEO$2UONSI 912 $aUON00055119 950 $aSIBA - SISTEMA BIBLIOTECARIO DI ATENEO$dSI ARA VI 063 $eSI AR 3098 7 063 996 $aTarih al-naqd al-'arabi$91151542 997 $aUNIOR