LEADER 05299nam 2200697Ia 450 001 9911020229103321 005 20200520144314.0 010 $a9786612292057 010 $a9781282292055 010 $a1282292056 010 $a9783527626687 010 $a3527626689 010 $a9783527626694 010 $a3527626697 035 $a(CKB)1000000000767195 035 $a(EBL)481485 035 $a(OCoLC)437416793 035 $a(SSID)ssj0000137586 035 $a(PQKBManifestationID)11159659 035 $a(PQKBTitleCode)TC0000137586 035 $a(PQKBWorkID)10088690 035 $a(PQKB)11399282 035 $a(MiAaPQ)EBC481485 035 $a(PPN)194575683 035 $a(Perlego)2757605 035 $a(EXLCZ)991000000000767195 100 $a20080107d2009 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aDetection of highly dangerous pathogens $emicroarray methods for BSL 3 and BSL 4 agents /$fedited by Tanja Kostic, Patrick Butaye, and Jacques Schrenzel 210 $aWeinheim $cWiley-VCH$d2009 215 $a1 online resource (193 p.) 300 $aDescription based upon print version of record. 311 08$a9783527322756 311 08$a3527322752 320 $aIncludes bibliographical references and index. 327 $aDetection of Highly Dangerous Pathogens: Microarray Methods for the Detection of BSL 3 and BSL 4 Agents; Contents; This Publication is Supported by COST; Preface; List of Contributors; 1 Introduction to Microarray-Based Detection Methods; 1.1 Introduction to Microarray Technology; 1.2 Technical Aspects of Microarray Technology; 1.2.1 Probes; 1.2.1.1 Genome Fragments; 1.2.1.2 PCR Products; 1.2.1.3 Oligonucleotide Probes; 1.2.2 Substrates for Printing; 1.2.2.1 Slides with Poly-L-lysine Coating; 1.2.2.2 Slides with Amino Silane Coating; 1.2.2.3 Slides with Aldehyde Coating 327 $a1.2.2.4 Slides with Epoxy Coating1.2.2.5 Proprietary Surface Chemistries; 1.2.2.6 Probe Spacers; 1.2.3 Targets for Microarray Analysis; 1.2.3.1 Target Amplifications and Sensitivity Issues; 1.2.3.2 Labeling of the Targets; 1.2.3.3 Hybridization and Wash Conditions; 1.2.4 Classical Commercially Available Microarray Formats; 1.2.4.1 Spotting Approaches; 1.2.4.2 In Situ Synthesis; 1.2.5 Alternative Methods for Improving Microarray-Based Detection Sensitivity; 1.2.5.1 Resonance-Light Scattering (RLS); 1.2.5.2 Planar-Waveguide Technology (PWT); 1.2.5.3 Liquid Arrays 327 $a1.2.5.4 Three-Dimensional Microarray Formats1.2.6 Marker Genes Used on MDMs; 1.3 Analysis and Quality Control Aspects; 1.4 Applications of Microarray Technology in Microbial Diagnostics; 1.4.1 Gene Expression Studies; 1.4.2 Comparative Genomic Hybridization (CGH); 1.4.3 Generic or Universal Microarrays; 1.4.4 Microarrays for Sequence Analysis; 1.4.5 Microbial Diagnostic Microarrays (MDMs); 1.5 Further Developments and New Perspectives Regarding Array Sensitivity and Specificity; 1.6 Conclusions; References; Part I: Methods; 2 Long Oligonucleotide Microarray-Based Microbial Detection 327 $a2.1 Introduction2.2 Method; 2.2.1 DNA Extraction; 2.2.2 ?29 Amplification; 2.2.3 Klenow Amplification/Labeling; 2.2.4 Probe and Slide Preparation; 2.2.5 Slide Processing Protocol (for Amino Surfaces); 2.2.6 Hybridization and Slide Washing; 2.2.7 Comments; 2.3 Our Test System and Results; 2.4 Conclusions; References; 3 Sequence-Specific End-Labeling of Oligonucleotides; 3.1 Introduction; 3.2 Probe Design; 3.3 Slide Preparation (Spotting); 3.4 Slide Processing Protocol ( for Aldehyde Surfaces); 3.5 DNA Extraction and PCR Amplification of the Targeted Gene 327 $a3.6 Shrimp Alkaline Phosphatase Treatment3.7 Labeling; 3.8 Hybridization and Slide Washing; 3.9 Data Analysis; 3.10 Costs; 3.11 Microarray for Detection of Pathogenic Bacteria; References; 4 Non-Cognate Approaches for Pathogen Detection on Microarrays; 4.1 Introduction; 4.2 Non-Cognate Hybridization System; 4.2.1 Concept; 4.2.2 Definition of the Optimal Probe Length; 4.2.3 Virtual Assessment of Array Performances (in Silico Experiments); 4.2.4 Array Manufacturing and Hybridization (Wet-Lab Experiments); 4.2.5 Analysis; 4.3 Perspectives; References; 5 Patterning Techniques for Array Platforms 327 $a5.1 Introduction 330 $aWritten by leading experts in the field as part of an interdisciplinary pan-European research program funded by the EU, the results provided in this booklet provide a unique and comprehensive overview of how microarray technology can be used in safely tracking the most highly dangerous pathogens. 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