LEADER 03145nam 2200649Ia 450 001 9911020168003321 005 20200520144314.0 010 $a9786610366590 010 $a9781280366598 010 $a1280366591 010 $a9780470244593 010 $a0470244593 010 $a9780471461036 010 $a0471461032 010 $a9780471223900 010 $a0471223905 035 $a(CKB)111056485581914 035 $a(EBL)152099 035 $a(OCoLC)475871949 035 $a(SSID)ssj0000204810 035 $a(PQKBManifestationID)11168594 035 $a(PQKBTitleCode)TC0000204810 035 $a(PQKBWorkID)10192119 035 $a(PQKB)11661517 035 $a(MiAaPQ)EBC152099 035 $a(Perlego)2764459 035 $a(EXLCZ)99111056485581914 100 $a20010214d2001 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aMolecular biology problem solver $ea laboratory guide /$fedited by Alan S. Gerstein 210 $aNew York $cWiley$dc2001 215 $a1 online resource (591 p.) 300 $aDescription based upon print version of record. 311 08$a9780471379720 311 08$a0471379727 320 $aIncludes bibliographical references and index. 327 $aMOLECULAR BIOLOGY PROBLEM SOLVER; Contents; Preface; Contributors; Chapter 1. Preparing for Success in the Laboratory; Chapter 2. Getting What You Need from a Supplier; Chapter 3. The Preparation of Buffers and Other Solutions: A Chemist's Perspective; Chapter 4. How to Properly Use and Maintain Laboratory Equipment; Chapter 5. Working Safely with Biological Samples; Chapter 6. Working Safely with Radioactive Materials; Chapter 7. DNA Purification; Chapter 8. RNA Purification; Chapter 9. Restriction Endonucleases; Chapter 10. Nucleotides, Oligonucleotides, and Polynucleotides; Chapter 11. PCR 327 $aChapter 12. ElectrophoresisChapter 13. Western Blotting; Chapter 14. Nucleic Acid Hybridization; Chapter 15. E. coli Expression Systems; Chapter 16. Eukaryotic Expression; Index 330 $aMost research in the life sciences involves a core set of molecular-based equipment and methods, for which there is no shortage of step-by-step protocols. Nonetheless, there remains an exceedingly high number of inquiries placed to commercial technical support groups, especially regarding problems. Molecular Biology Problem Solver: A Laboratory Guide asks the reader to consider crucial questions, such as:Have you selected the most appropriate research strategy?Have you identified the issues critical to your successful application of a technique?Are you familiar 606 $aMolecular biology$xMethodology 606 $aMolecular biology$vLaboratory manuals 615 0$aMolecular biology$xMethodology. 615 0$aMolecular biology 676 $a572.8 676 $a572.8078 701 $aGerstein$b Alan S.$f1957-$01837915 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9911020168003321 996 $aMolecular biology problem solver$94416771 997 $aUNINA