LEADER 05410nam 2200673Ia 450 001 9911019938903321 005 20200520144314.0 010 $a9786610723423 010 $a9781280723421 010 $a1280723424 010 $a9783527608096 010 $a3527608095 010 $a9783527607938 010 $a3527607935 035 $a(CKB)1000000000375911 035 $a(EBL)481832 035 $a(OCoLC)744977407 035 $a(SSID)ssj0000296023 035 $a(PQKBManifestationID)11253801 035 $a(PQKBTitleCode)TC0000296023 035 $a(PQKBWorkID)10320884 035 $a(PQKB)11479163 035 $a(MiAaPQ)EBC481832 035 $a(Perlego)2759834 035 $a(EXLCZ)991000000000375911 100 $a20050527d2006 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aExpression and analysis of recombinant ion channels $efrom structural studies to pharmacological screening /$fedited by Jeffrey J. Clare, Derek J. Trezise 210 $aWeinheim ;$a[Chichester] $cWiley-VCH$dc2006 215 $a1 online resource (305 p.) 300 $aDescription based upon print version of record. 311 08$a9783527312092 311 08$a3527312099 320 $aIncludes bibliographical references and index. 327 $aExpression and Analysis of Recombinant Ion Channels; Contents; Preface; List of Contributors; Color Plates; 1 Expression of Ion Channels in Xenopus Oocytes; 1.1 Introduction; 1.2 Advantages and Disadvantages of Xenopus Oocytes; 1.3 Procedures for Using Oocytes; 1.4 Types of Analyses; 1.4.1 Electrophysiological Analysis; 1.4.1.1 Two-electrode Whole Cell Voltage-clamp; 1.4.1.2 Cut-open Oocyte Voltage-clamp; 1.4.1.3 Macropatch Clamp; 1.4.1.4 Single Channel Analysis; 1.4.2 Biochemical Analysis; 1.4.3 Compound Screening; 1.4.3.1 Serial Recording Using the Roboocyte 327 $a1.4.3.2 Parallel Recording Using the OpusXpress1.5 Examples of Use; 1.5.1 Characterization of cDNA Clones for a Channel; 1.5.2 Structure-Function Correlations; 1.5.3 Studies of Human Disease Mutations; 1.6 Conclusions; Acknowledgments; References; 2 Molecular Biology Techniques for Structure - Function Studies of Ion Channels; 2.1 Introduction; 2.2 Methods for cDNA Subcloning; 2.2.1 Conventional Sub-cloning Using Restriction Enzymes and DNA Ligase; 2.2.2 PCR-based cDNA Sub-cloning; 2.2.3 Sub-cloning cDNA through Site-specific Recombination; 2.3 Generation of Chimeric Channel cDNAs 327 $a2.3.1 Use of Restriction Enzymes to Generate Chimeric Channel cDNAs2.3.2 PCR-mediated Overlap Extension for Chimera Generation; 2.3.3 PCR-mediated Integration or Replacement of cDNA Fragments; 2.4 Site-directed Mutagenesis; 2.4.1 Examples of the Use of Site-directed Mutagenesis; 2.4.2 Modification of the QuikChange Method for the Replacement of cDNA Fragments; 2.5 Epitope-tagged Channels and Fusion Partners; 2.6 Channel Subunit Concatamers; 2.7 Concluding Remarks; References; 3 Unnatural Amino Acids as Probes of Ion Channel Structure - Function and Pharmacology; 3.1 Introduction 327 $a3.2 Unnatural Amino Acid Mutagenesis Methodology3.3 Unnatural Amino Acid Mutagenesis for Ion Channel Studies; 3.4 Structure-Function Example Studies; 3.4.1 Nicotinic Acetylcholine Receptor; 3.4.2 Drug Interactions with the hERG Voltage-gated Potassium Ion Channel; 3.5 Other Uses of Unnatural Amino Acids as Probes of Protein Structure and Function; 3.6 Conclusions; Acknowledgements; References; 4 Functional Expression of Ion Channels in Mammalian Systems; 4.1 Introduction; 4.2 cDNA Cloning and Manipulation; 4.3 Choice of Host Cell Background 327 $a4.4 Post-translational Processing of Heterologous Expressed Ion Channels4.5 Cytotoxicity; 4.6 Transient Expression Systems; 4.6.1 "Standard" Transient Expression; 4.6.2 Viral Expression Systems; 4.7 Stable Expression of Ion Channels; 4.7.1 Bicistronic Expression Systems; 4.7.2 Stable Expression of Multiple Subunits; 4.7.3 Inducible Expression; 4.8 Summary; Acknowledgements; References; 5 Analysis of Electrophysiological Data; 5.1 Overview; 5.2 Introduction; 5.3 Expression Systems and Related Recording Techniques; 5.3.1 Expression in Xenopus Oocytes; 5.3.2 Expression in Mammalian Cells 327 $a5.3.3 Leak and Capacitance Subtraction 330 $aFilling the gap created over the past five years, during which many new techniques have entered the market, this book is directed at both the new and the experienced ion channel researcher wishing to learn more about the considerations and methods for studying recombinant ion channels. These latest developments are covered here for the first time, contributed by editors and authors working for major pharmaceutical companies and who routinely apply these techniques in their daily work. The first three chapters cover the use of the Xenopus oocyte expression system for structure-function stud 606 $aIon channels 606 $aBiological transport, Active 615 0$aIon channels. 615 0$aBiological transport, Active. 676 $a572.3 701 $aClare$b Jeffrey J$01840474 701 $aTrezise$b Derek J$01840475 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9911019938903321 996 $aExpression and analysis of recombinant ion channels$94420033 997 $aUNINA LEADER 03690nam 22007575 450 001 9911018744503321 005 20250726130251.0 010 $a981-9500-11-7 024 7 $a10.1007/978-981-95-0011-6 035 $a(CKB)39766967900041 035 $a(DE-He213)978-981-95-0011-6 035 $a(MiAaPQ)EBC32265638 035 $a(Au-PeEL)EBL32265638 035 $a(EXLCZ)9939766967900041 100 $a20250726d2025 u| 0 101 0 $aeng 135 $aur||||||||||| 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aAdvanced Intelligent Computing Technology and Applications $e21st International Conference, ICIC 2025, Ningbo, China, July 26?29, 2025, Proceedings, Part XI /$fedited by De-Shuang Huang, Chuanlei Zhang, Qinhu Zhang, Yijie Pan 205 $a1st ed. 2025. 210 1$aSingapore :$cSpringer Nature Singapore :$cImprint: Springer,$d2025. 215 $a1 online resource (XXI, 545 p. 213 illus., 192 illus. in color.) 225 1 $aCommunications in Computer and Information Science,$x1865-0937 ;$v2574 311 08$a981-9500-00-1 330 $aThe 12-volume set CCIS 2564-2575, together with the 28-volume set LNCS/LNAI/LNBI 15842-15869, constitutes the refereed proceedings of the 21st International Conference on Intelligent Computing, ICIC 2025, held in Ningbo, China, during July 26-29, 2025. The 523 papers presented in these proceedings books were carefully reviewed and selected from 4032 submissions. This year, the conference concentrated mainly on the theories and methodologies as well as the emerging applications of intelligent computing. Its aim was to unify the picture of contemporary intelligent computing techniques as an integral concept that highlights the trends in advanced computational intelligence and bridges theoretical research with applications. 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