LEADER 05211nam 2200649Ia 450 001 9911019249303321 005 20200520144314.0 010 $a9786611239268 010 $a9781281239266 010 $a1281239267 010 $a9783527612307 010 $a3527612300 010 $a9783527612314 010 $a3527612319 035 $a(CKB)1000000000375888 035 $a(EBL)482134 035 $a(OCoLC)609855542 035 $a(SSID)ssj0000303617 035 $a(PQKBManifestationID)11263557 035 $a(PQKBTitleCode)TC0000303617 035 $a(PQKBWorkID)10275996 035 $a(PQKB)11526391 035 $a(MiAaPQ)EBC482134 035 $a(Perlego)2772077 035 $a(EXLCZ)991000000000375888 100 $a20050323d2005 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aMore practical problem solving in HPLC /$fStavros Kromidas ; with contributions by Friedrich Mandel, Jurgen Maier-Rosenkranz and Hans-Joachim Kuss 210 $aWeinheim ;$a[Great Britain] $cWiley-VCH$dc2005 215 $a1 online resource (312 p.) 300 $aIncludes index. 311 08$a9783527311132 311 08$a3527311130 327 $aMore Practical Problem Solving in HPLC; Foreword; Contents; Preface; The Structure of the Book; Part 1 (general section); Part 2 (specific questions); In Lieu of an Introduction; Chromatography - and more - Crossword; Across; Down; An HPLC-Quiz; An HPLC Tale; The Tale of Peaky and Chromy; 1 HPLC Tips; 1.1 Stationary Phases and Columns; Tip No. 01 "It improves with age" is a rule that applies to port and sometimes to red wine, but how about your C(18) column?; Tip No. 02 Optimization via column parameters - what works best? 327 $aTip No. 03 Can selectivity always be put down to chemical interactions with the stationary phase?Tip No. 04 A matter of perspective . . . Or: Selectivity and peak symmetry of basic compounds using reversed-phase packing materials; Tip No. 05 Separation of isomers; Tip No. 06 When should I use a "polar" C(18) phase?; Tip No. 07 Are polar RP-C(18) phases more suitable for the separation of polar analytes than non-polar phases?; Tip No. 08 What about non-endcapped phases - are they a thing of the past?; Tip No. 09 How can I separate acids using RP C(18)? 327 $aTip No. 10 The nitrile phase - some like it polarTip No. 11 The selectivity of RP columns; 1.2 Buffers, pH Value; Tip No. 12 Does it always have to be potassium phosphate?; Tip No. 13 UV cut-off of buffer solutions; Tip No. 14 Sources of errors when using buffers; Tip No. 15 The drawbacks of using buffers; Tip No. 16 Why is the pH value so important, and what does it do?; Tip No. 17 Why does the pH value shift even though I am using the correct buffer and the buffer capacity is sufficient?; Tip No. 18 Changes to the pH value in the eluent: the extent of the shift and the reasons behind it 327 $aTip No. 19 An unintentional pH shift and its consequencesTip No. 20 RP separations in the alkaline medium; Tip No. 21 Separation of basic and acidic compounds contained in the same sample; 1.3 Optimization, Peak Homogeneity; Tip No. 22 The peaks appear too soon - what can be done?; Tip No. 23 What can I do if the peaks elute late?; Tip No. 24 Quick optimization of an existing gradient method; Tip No. 25 Increasing efficiency - often the fast track to success; Tip No. 26 Additives to the eluent; Tip No. 27 Separating the unknown - where shall I begin? 327 $aTip No. 28 Separation of an unknown sample using a reversed-phase C(18) column - how do I go about it?Tip No. 29 Developing an RP separation - the two-day-method; Part 1: Choice of column and eluent; Tip No. 30 Developing an RP separation - the two-day method; Part 2: Fine-tuning of the separation; Tip No. 31 Quick check on peak homogeneity; Tip No. 32 Quick check on peak homogeneity; Tip No. 33 Tied to a standard operating procedure - how can a bad separation be improved further?; Tip No. 34 More elaborate measures to check peak homogeneity; Tip No. 35 First easily digestible tip 327 $aTip No. 36 Second easily digestible tip 330 $aA unique approach to solving HPLC problems.Everyone who bought ""Problem Solving in HPLC"" by Stavros Kromidas will equally benefit from nearly 100 new practical examples for optimization, trouble-shooting, and instrument performance given in this sequel.The author provides- guidance for selecting and evaluating methods, intstruments and columns,- practical help with everyday trouble-shooting,- advice for optimizing separations, always explaining the reason why.In each case the problem, the solution and the conclusions are presented over a maximum of 4 pages, and in add 606 $aHigh performance liquid chromatography 606 $aPhase partition 615 0$aHigh performance liquid chromatography. 615 0$aPhase partition. 676 $a543.84 700 $aKromidas$b Stavros$01604367 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9911019249303321 996 $aMore practical problem solving in HPLC$94421112 997 $aUNINA