LEADER 01251nam 2200361 450 001 9910711947703321 005 20190308154616.0 035 $a(CKB)5470000002488615 035 $a(OCoLC)1089451135 035 $a(EXLCZ)995470000002488615 100 $a20190308d2015 ua 0 101 0 $aeng 135 $aur||||||||||| 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aDATA Act: USASpending.gov refreshed, but data quality issues remain 210 1$a[Washington, D.C.] :$cOffice of Inspector General, Department of the Treasury,$d2015. 215 $a1 online resource (i, 20 pages) 225 1 $aAudit report ;$vOIG-16-023 300 $a"December 22, 2015." 320 $aIncludes bibliographical references. 517 $aDATA Act 606 $aAdministrative agencies$zUnited States$xAccounting 606 $aTransparency in government$xComputer networks$zUnited States 615 0$aAdministrative agencies$xAccounting. 615 0$aTransparency in government$xComputer networks 801 0$bGPO 801 1$bGPO 906 $aBOOK 912 $a9910711947703321 996 $aDATA Act: USASpending.gov refreshed, but data quality issues remain$93328896 997 $aUNINA LEADER 04025nam 22006615 450 001 9911015636003321 005 20250922173415.0 010 $a1-0716-4124-7 024 7 $a10.1007/978-1-0716-4124-8 035 $a(MiAaPQ)EBC32206303 035 $a(Au-PeEL)EBL32206303 035 $a(CKB)39625841100041 035 $a(DE-He213)978-1-0716-4124-8 035 $a(OCoLC)1564376367 035 $a(EXLCZ)9939625841100041 100 $a20250710d2025 u| 0 101 0 $aeng 135 $aurcnu|||||||| 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aBiophysical Chemistry of Proteins $eAn Introduction to Laboratory Methods /$fby Engelbert Buxbaum 205 $a2nd ed. 2025. 210 1$aNew York, NY :$cSpringer US :$cImprint: Springer,$d2025. 215 $a1 online resource (911 pages) 225 0 $aBiomedical and Life Sciences Series 311 08$a1-0716-4123-9 327 $aAnalytical techniques -- Microscopy -- Single molecule techniques -- Preparation of cells and tissues for microscopy -- Principles of optical spectroscopy -- Photometry -- Fluorimetry -- Chemiluminescence -- Electrophoresis -- Immunological methods -- Isotope techniques -- Purification of proteins -- Homogenisation and fractionisation of cells and tissues -- Isolation of organelles -- Precipitation methods -- Chromatography -- Membrane proteins -- Determination of protein concentration -- Cell culture -- Protein modification and inactivation -- General technical remarks -- Amine-reactive reagents -- Thiol- and disulphide reactive reagents -- Reagents for other groups -- Cross-linkers -- Detection methods -- Spontaneous reactions in proteins -- Protein size and shape -- Centrifugation -- Osmotic pressure -- Diffusion -- Viscosity -- Non-resonant interactions with electromagnetic waves -- Protein structure -- Protein sequencing -- Synthesis of peptides -- Protein secondary structure -- Structure of protein-ligand complexes -- 3D-structures -- Folding and unfolding of proteins -- Enzyme kinetics -- Steady-state kinetics -- Leaving the steady state: Analysis of progress curves -- Reaction velocities -- Isotope effects -- Isotope exchange -- Protein-ligand interactions -- General conditions for interpretable results -- Binding equations -- Methods to measure binding equilibria -- Temperature effects on binding equilibrium and reaction rate -- Industrial enzymology -- Industrial enzyme use -- Immobilised enzymes -- Special statistics -- Quality control -- Testing whether or not a model fits the data -- Appendix -- List of symbols -- Greek alphabet -- Properties of electrophoretic buffers -- Bond properties -- Acronyms. 330 $aThis textbook, designed for all scientists interested in protein research, provides a thorough overview of laboratory methods for the biophysical chemistry of proteins. This new edition, completely restructured and expanded for ease of learning, includes sections on analytical techniques, working with proteins, protein size and shape, protein structure, enzyme kinetics, industry enzymology, and a new section on special statistics. 606 $aProteins 606 $aProteins$xSynthesis 606 $aProteins 606 $aBiochemistry 606 $aChemistry 606 $aProtein Biochemistry 606 $aProtein Synthesis and Translation 606 $aProteins 606 $aBiochemistry 606 $aChemistry 615 0$aProteins. 615 0$aProteins$xSynthesis. 615 0$aProteins. 615 0$aBiochemistry. 615 0$aChemistry. 615 14$aProtein Biochemistry. 615 24$aProtein Synthesis and Translation. 615 24$aProteins. 615 24$aBiochemistry. 615 24$aChemistry. 676 $a572.6 700 $aBuxbaum$b Engelbert$01061033 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9911015636003321 996 $aBiophysical Chemistry of Proteins$94408062 997 $aUNINA