LEADER 00990nas# 22002771i 450 001 UON00048973 005 20231205102217.428 100 $a20020107a2000 |0itac50 ba 101 $aeng 102 $aFR 105 $a|||| 1|||| 110 $aaU||||||||| 200 1 $aPattrika$eBulletin of the French Research Institutes in India 205 $a[S.l. : s.n.] 207 $av. ; 30 cm 210 $aPeriodicità sconosciuta 215 $aDescrizione basata su: anno 2000 n. 3 (maggio) 686 $aFR$cFRANCIA - PERIODICI$2A 686 $aDONO$cPERIODICI - DONO$2A 712 $as.n.$3UONV247931$4650 801 $aIT$bSOL$c20240220$gRICA 899 $aSIBA - SISTEMA BIBLIOTECARIO DI ATENEO$2UONSI$42000 *3*$cPer Misc 132 ; 912 $aUON00048973 950 $aSIBA - SISTEMA BIBLIOTECARIO DI ATENEO$bSI 2000 *3*$dSI A Per Misc 132 2000 $eSI SA 93152 7 2000 996 $aPattrika$91320517 997 $aUNIOR LEADER 03939nam 22006375 450 001 9911015636003321 005 20250712074743.0 010 $a1-0716-4124-7 024 7 $a10.1007/978-1-0716-4124-8 035 $a(MiAaPQ)EBC32206303 035 $a(Au-PeEL)EBL32206303 035 $a(CKB)39625841100041 035 $a(DE-He213)978-1-0716-4124-8 035 $a(EXLCZ)9939625841100041 100 $a20250710d2025 u| 0 101 0 $aeng 135 $aurcnu|||||||| 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aBiophysical Chemistry of Proteins $eAn Introduction to Laboratory Methods /$fby Engelbert Buxbaum 205 $a2nd ed. 2025. 210 1$aNew York, NY :$cSpringer US :$cImprint: Springer,$d2025. 215 $a1 online resource (911 pages) 311 08$a1-0716-4123-9 327 $aAnalytical techniques -- Microscopy -- Single molecule techniques -- Preparation of cells and tissues for microscopy -- Principles of optical spectroscopy -- Photometry -- Fluorimetry -- Chemiluminescence -- Electrophoresis -- Immunological methods -- Isotope techniques -- Purification of proteins -- Homogenisation and fractionisation of cells and tissues -- Isolation of organelles -- Precipitation methods -- Chromatography -- Membrane proteins -- Determination of protein concentration -- Cell culture -- Protein modification and inactivation -- General technical remarks -- Amine-reactive reagents -- Thiol- and disulphide reactive reagents -- Reagents for other groups -- Cross-linkers -- Detection methods -- Spontaneous reactions in proteins -- Protein size and shape -- Centrifugation -- Osmotic pressure -- Diffusion -- Viscosity -- Non-resonant interactions with electromagnetic waves -- Protein structure -- Protein sequencing -- Synthesis of peptides -- Protein secondary structure -- Structure of protein-ligand complexes -- 3D-structures -- Folding and unfolding of proteins -- Enzyme kinetics -- Steady-state kinetics -- Leaving the steady state: Analysis of progress curves -- Reaction velocities -- Isotope effects -- Isotope exchange -- Protein-ligand interactions -- General conditions for interpretable results -- Binding equations -- Methods to measure binding equilibria -- Temperature effects on binding equilibrium and reaction rate -- Industrial enzymology -- Industrial enzyme use -- Immobilised enzymes -- Special statistics -- Quality control -- Testing whether or not a model fits the data -- Appendix -- List of symbols -- Greek alphabet -- Properties of electrophoretic buffers -- Bond properties -- Acronyms. 330 $aThis textbook, designed for all scientists interested in protein research, provides a thorough overview of laboratory methods for the biophysical chemistry of proteins. This new edition, completely restructured and expanded for ease of learning, includes sections on analytical techniques, working with proteins, protein size and shape, protein structure, enzyme kinetics, industry enzymology, and a new section on special statistics. 606 $aProteins 606 $aProteins$xSynthesis 606 $aProteins 606 $aBiochemistry 606 $aChemistry 606 $aProtein Biochemistry 606 $aProtein Synthesis and Translation 606 $aProteins 606 $aBiochemistry 606 $aChemistry 615 0$aProteins. 615 0$aProteins$xSynthesis. 615 0$aProteins. 615 0$aBiochemistry. 615 0$aChemistry. 615 14$aProtein Biochemistry. 615 24$aProtein Synthesis and Translation. 615 24$aProteins. 615 24$aBiochemistry. 615 24$aChemistry. 676 $a572.6 700 $aBuxbaum$b Engelbert$01061033 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9911015636003321 996 $aBiophysical Chemistry of Proteins$94408062 997 $aUNINA