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010   $a9789819613014
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024 7 $a10.1007/978-981-96-1301-4
035   $a(CKB)37203792500041
035   $a(MiAaPQ)EBC31878994
035   $a(Au-PeEL)EBL31878994
035   $a(DE-He213)978-981-96-1301-4
035   $a(OCoLC)1484883055
035   $a(EXLCZ)9937203792500041
100   $a20250114d2025      u|         0
101 0 $aeng
135   $aur|||||||||||
181   $ctxt$2rdacontent
182   $cc$2rdamedia
183   $acr$2rdacarrier
200 10$aDevelopment of Mirror-Image VHHs with Less Immunogenicity /$fby Keisuke Aoki
205   $a1st ed. 2025.
210  1$aSingapore :$cSpringer Nature Singapore :$cImprint: Springer,$d2025.
215   $a1 online resource (140 pages)
225 1 $aSpringer Theses, Recognizing Outstanding Ph.D. Research,$x2190-5061
311 08$a9789819613007 
311 08$a9819613000 
327   $aChapter 1.Introduction -- Chapter 2.Chemical Synthesis of Mirror-Image VHHs and Evaluation of Their Immunogenicity -- Chapter 3.Identification of a Mirror-Image VHH against Vascular Endothelial Growth Factor -- Chapter 4.Synthetic Study of Full-Length Hepatitis B Virus Core Protein and Its Capsid Assembly -- Chapter 5.Chemical Synthesis of Interleukin-6 for Mirror-Image VHH Discovery -- Chapter 6.Conclusions.
330   $aThis book outlines the chemical synthesis of therapeutic targets and a screening process for unexplored mirror-image single-domain antibodies (D-VHH). This book first describes the chemical synthesis of model VHH and the characteristics of both enantiomeric VHHs, including binding activity, biodistribution and immunogenicity. Immunogenicity testing in mice demonstrated that administration of conventional L-VHH induced the generation of anti-drug antibodies while D-VHH-binding antibodies were not observed in D-VHH-immunized mice. Second, it is explained that the T7-phage-based mirror-image screening system was developed to identify D-VHH antibody fragments capable of binding to vascular endothelial growth factors. Additionally, this book details the chemical synthesis of full-length hepatitis B virus core protein and interleukin-6 for the application of exploring D-VHHs and other mirror-image molecules. Demonstrating that it is possible to develop a D-VHH that preferentially binds the native target protein and exhibits reduced immunogenicity, this book is useful for anyone interested in developing alternative biotherapeutics with reduced immunogenicity by combining chemical and biological approaches.
410  0$aSpringer Theses, Recognizing Outstanding Ph.D. Research,$x2190-5061
606   $aChemistry
606   $aPharmacy
606   $aProteins
606   $aImmunogenetics
606   $aChemical Synthesis
606   $aPharmacy
606   $aProteins
606   $aImmunogenetics
615  0$aChemistry.
615  0$aPharmacy.
615  0$aProteins.
615  0$aImmunogenetics.
615 14$aChemical Synthesis.
615 24$aPharmacy.
615 24$aProteins.
615 24$aImmunogenetics.
676   $a541.39
700   $aAoki$b Keisuke$01785138
801  0$bMiAaPQ
801  1$bMiAaPQ
801  2$bMiAaPQ
906   $aBOOK
912   $a9910983338303321
996   $aDevelopment of Mirror-Image VHHs with Less Immunogenicity$94316765
997   $aUNINA