LEADER 06244nam 22006013u 450 001 9910973676403321 005 20240501071021.0 010 $a9780444632661 010 $a0444632662 010 $a9780444632654 010 $a0444632654 035 $a(CKB)3710000000761898 035 $a(EBL)4613243 035 $a(NjHacI)993710000000761898 035 $a(PPN)198054394 035 $a(FR-PaCSA)88835245 035 $a(MiAaPQ)EBC4613243 035 $a(FRCYB88835245)88835245 035 $a(BIP)46841789 035 $a(EXLCZ)993710000000761898 100 $a20160808d2015|||| u|| | 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aStem Cell Manufacturing 205 $a1st ed. 210 $aOxford $cElsevier Science$d2015 210 1$aOxford :$cElsevier Science,$d2015. 215 $a1 online resource (342 p.) 300 $aDescription based upon print version of record. 327 $aFront Cover; STEM CELL MANUFACTURING; STEM CELL MANUFACTURING; Copyright; CONTENTS; LIST OF CONTRIBUTORS; INTRODUCTION; 1 - Genetic Engineering in Stem Cell Biomanufacturing ; 1.1 INTRODUCTION; 1.2 GENETIC MANIPULATION APPROACHES IN HUMAN PLURIPOTENT STEM CELLS; 1.2.1 Transgenic Approaches; 1.2.2 Knock-In and Knock-Out Approaches; 1.2.2.1 Zinc-Finger Nucleases; 1.2.2.2 Transcription Activator-Like Effector Nucleases; 1.2.2.3 Clustered Regularly Interspaced Short Palindromic Repeat/Cas9; 1.2.3 Bacterial Artificial Chromosomes; 1.3 APPLICATIONS 327 $a1.3.1 Genetic Labeling for Cell Identification and Cell Tracking1.3.2 Gene Alteration for Directed Differentiation; 1.3.3 Gene Disruption for Functional Investigation; 1.3.4 Gene Correction for Function Restoration; 1.4 DELIVERY METHODS; 1.4.1 Transfection; 1.4.2 Nucleofection; 1.4.3 Viral Transduction; 1.5 CONCLUSIONS; ACKNOWLEDGMENTS; REFERENCES; 2 - Biomechanics in Stem Cell Manufacturing; 2.1 INTRODUCTION; 2.2 CELLULAR BIOMECHANICS; 2.2.1 Biomechanical Cues; 2.2.2 Shear Forces and Differentiated Cells; 2.2.3 Shear Forces and Pluripotent Stem Cells 327 $a2.3 SCALE UP TOWARD PRODUCTION-LEVEL BIOREACTORS2.4 BIOMANUFACTURING CELLS FOR THERAPIES; 2.4.1 Pluripotent Stem Cells; 2.4.2 Cardiomyocytes; 2.4.3 Endothelial Cells; 2.5 CONCLUSION; REFERENCES; 3 - Bioreactor Engineering Fundamentals for Stem Cell Manufacturing ; 3.1 INTRODUCTION; 3.2 STIRRED BIOREACTOR BASICS; 3.3 SPECIAL FEATURES OF STIRRED BIOREACTORS FOR HMSC CULTURE ON MICROCARRIERS; 3.3.1 Introduction; 3.3.2 Preparing the Bioreactor for Culture; 3.3.3 Medium and Medium Exchange; 3.3.4 Microcarrier Selection; 3.3.5 Cell and Microcarrier Concentrations; 3.3.6 Attachment Protocol 327 $a3.3.7 Use of Coatings to Enhance Attachment3.3.8 The Minimum Speed for Suspension, NJS and Associated Mean Specific Energy Dissipation Rate, ?T;? 3.3.8.1 General Aspects; 3.3.8.2 NJS Considerations in hMSC Culture; 3.3.9 Oxygen Demand, Mass Transfer, and Optimum Dissolved Oxygen; 3.3.9.1 General Considerations; 3.3.9.2 Application to hMSC Culture; 3.3.10 Fluid Dynamically Generated Stresses and Cell Proliferation; 3.3.10.1 General Considerations; 3.3.10.2 Application to hMSC Culture; 3.3.11 Fluid Dynamically Generated Stresses and Their Application to Cell Harvesting; 3.4 FUTURE ISSUES 327 $a3.4.1 Increasing Cell Density3.4.2 Oxygen Demand and Mass Transfer at Higher Cell Density-Sparging and Higher Agitator Speeds; 3.4.3 Carbon Dioxide, Osmolality, and pH; 3.4.4 Human-Induced and Embryonic Pluripotent Stem Cells; 3.5 CONCLUSIONS; NOMENCLATURE; REFERENCES; 4 - Microcarrier Culture Systems for Stem Cell Manufacturing ; 4.1 OVERVIEW; 4.1.1 Historical Perspective; 4.2 MICROCARRIER TECHNOLOGY; 4.2.1 Types of Microcarriers; 4.2.2 Properties of Microcarriers Required for Cell Culturing; 4.2.3 Advantages of Using Microcarrier Culture Systems for Cell Manufacturing 327 $a4.3 SCALABLE CULTURE SYSTEMS FOR ADHERENT STEM CELLS 330 $aStem Cell Manufacturing discusses the required technologies that enable the transfer of the current laboratory-based practice of stem cell tissue culture to the clinic environment as therapeutics, while concurrently achieving control, reproducibility, automation, validation, and safety of the process and the product.The advent of stem cell research unveiled the therapeutic potential of stem cells and their derivatives and increased the awareness of the public and scientific community for the topic. The successful manufacturing of stem cells and their derivatives is expected to have a positive impact in the society since it will contribute to widen the offer of therapeutic solutions to the patients. Fully defined cellular products can be used to restore the structure and function of damaged tissues and organs and to develop stem cell-based cellular therapies for the treatment of cancer and hematological disorders, autoimmune and other inflammatory diseases and genetic disorders.- Presents the first 'Flowchart' of stem cell manufacturing enabling easy understanding of the various processes in a sequential and coherent manner- Covers all bioprocess technologies required for the transfer of the bench findings to the clinic including the process components: cell signals, bioreactors, modeling, automation, safety, etc.- Presents comprehensive coverage of a true multidisciplinary topic by bringing together specialists in their particular area- Provides the basics of the processes and identifies the issues to be resolved for large scale cell culture by the bioengineer- Addresses the critical need in bioprocessing for the successful delivery of stem cell technology to the market place by involving professional engineers in sections of the book 606 $aStem cells 615 0$aStem cells. 676 $a616.02774 700 $aCabral$b Joaquim S$01873820 701 $ada Silva$b Claudia Lobato$01795425 701 $aChase$b Lucas G$01695780 701 $aDiogo$b Maria Margarida$01795426 801 0$bAU-PeEL 801 1$bAU-PeEL 801 2$bAU-PeEL 906 $aBOOK 912 $a9910973676403321 996 $aStem Cell Manufacturing$94484065 997 $aUNINA