LEADER 01305nam a2200325 i 4500 001 991003738879707536 005 20250514143924.0 008 980519s1989 it er |01 0 ita d 035 $ab10549353-39ule_inst 035 $aEXGIL126191$9ExL 040 $aBiblioteca Interfacoltà$bita$dSocioculturale Scs 041 0 $aita 082 04$a195$223 100 1 $aGentile, Giovanni$d<1875-1944>$0266892 245 10$aOpere complete di Giovanni Gentile / a cura di Hervè A. Cavallera 246 18$aOpere 260 $aFirenze :$bLe lettere,$c1989 300 $avolumi ;$c22 cm 505 00$g41. :$tLa riforma della scuola in Italia. -$g3. ed. rivista e accresciuta. -$g505 p. 505 00$g43. :$tGuerra e fede. -$g3. ed. rivista e accresciuta. -$gXI, 309 p. 505 00$g44. :$tDopo la vittoria. -$g2. ed. rivista e accresciuta. -$gVIII, 246 p. 600 14$aGentle, Giovanni$vOpere 700 1 $aCavallera, Hervé Antonio 740 3 $aLa riforma della scuola in Italia 907 $a.b10549353$b02-04-14$c27-06-02 912 $a991003738879707536 945 $aLE002 Fil. XVII D 1$g1$i2002000004529$lle002$o-$pE0.00$q-$rl$s-$t0$u2$v0$w2$x0$y.i10631276$z27-06-02 996 $aOpere complete di Giovanni Gentile$9216322 997 $aUNISALENTO 998 $ale002$b01-01-98$cm$da$e-$fita$git$h0$i1 LEADER 04428nam 2200817 n 450 001 9910968095703321 005 20251116191803.0 010 $a0-19-770144-2 010 $a1-4294-1517-7 010 $a9786610481729 010 $a9786610427888 010 $a1-280-48172-2 010 $a1-280-42788-4 010 $a0-19-803251-X 010 $a1-60256-787-5 024 7 $a10.1093/oso/9780195142266.001.0001 035 $a(CKB)1000000000363096 035 $a(SSID)ssj0000188298 035 $a(PQKBManifestationID)11182826 035 $a(PQKBTitleCode)TC0000188298 035 $a(PQKBWorkID)10143911 035 $a(PQKB)11785723 035 $a(Au-PeEL)EBL279527 035 $a(CaPaEBR)ebr10142389 035 $a(OCoLC)935262024 035 $a(Au-PeEL)EBL4963511 035 $a(CaONFJC)MIL48172 035 $a(OCoLC)60314677 035 $a(MiAaPQ)EBC279527 035 $a(OCoLC)1406788694 035 $a(StDuBDS)9780197701447 035 $a(MiAaPQ)EBC4963511 035 $a(MiAaPQ)EBC4704593 035 $a(Au-PeEL)EBL4704593 035 $a(CaONFJC)MIL42788 035 $a(EXLCZ)991000000000363096 100 $a20040301e20232004 |y | 101 0 $aeng 135 $aurcn||||||||| 181 $ctxt 182 $cc 183 $acr 200 02$aA laboratory guide to the mammalian embryo /$fedited by David K. Gardner, Michelle Lane, Andrew J. Watson 205 $a1st ed. 210 1$aOxford :$cOxford University Press,$d2023. 215 $axiv, 394 p. $cill. (some col.) 225 1 $aOxford scholarship online 300 $aPreviously issued in print: 2004. 311 08$a0-19-517151-9 311 08$a0-19-514226-8 320 $aIncludes bibliographical references and index. 327 $aIntro -- Contents -- Contributors -- Abbreviations -- 1. Follicle Development in Vitro -- 2. Preparation of Gametes, in Vitro Maturation, in Vitro Fertilization, and Embryo Recovery and Transfer -- 3. Culture of the Mammalian Preimplantation Embryo -- 4. Assessment of Preimplantation Embryo Development and Viability -- 5. Micromanipulation of Gametes -- 6. Micromanipulation: Biopsy -- 7. Analysis of Intracellular Ions in Embryos: pH and Calcium -- 8. Assessment of Nutrient Uptake, Metabolite Production, and Enzyme Activity -- 9. Metabolic Pathway Activity -- 10. Confocal Imaging of Structural Molecules in Mammalian Gametes -- 11. Fluorescent in situ Hybridisation for Detection of Aneuploidy in Single Human Blastomeres for Preimplantation Genetic Diagnosis -- 12. Assessment of Ploidy, Telomere Length, and Telomerase Activity in Oocytes and Embryos -- 13. Microscale RNA Isolation from Mammalian Embryos -- 14. Relative mRNA Transcript Abundance in Early Embryos by Reverse Transcription-Polymerase Chain Reaction -- 15. Characterization of Novel Genes during Early Development by Application of Differential Display RT-PCR -- 16. Two-Dimensional Protein Gel Database Analysis of Embryos, Oocytes, and Oocyte-Associated Granulosa Cells -- 17. Antisense Disruption of Gene Expression in the Preimplantation Embryo -- 18. Gene Subtraction and Analysis -- 19. Analysis of Apoptosis in the Preimplantation Embryo -- 20. Cytoplasmic Signaling and Cell Cycle Control in the Mouse Egg and Embryo -- 21. Human Embryonic Stem Cells -- 22. Spermatogonial Transplantation -- 23. Somatic Cell Nuclear Transfer -- 24. Cryobiology: Slow Freezing Vitrification of Embryos -- Index. 330 8 $aDrawing from recent work with embryos from rodents, domestic animals, and primates, this practical guide describes the theory and protocols for performing biochemical, microscopic, and genetic techniques that have been developed and applied at the single-cell level. The techniques are the result of both new research and updates to established proce. 410 0$aOxford scholarship online. 606 $aEmbryology, Experimental$vLaboratory manuals 606 $aEmbryology, Human$vLaboratory manuals 615 0$aEmbryology, Experimental 615 0$aEmbryology, Human 676 $a571.8/619 702 $aGardner$b David K. 702 $aLane$b Michelle 702 $aWatson$b Andrew J$g(Andrew John),$f1959- 801 0$bDNLM/DLC 801 1$bDLC 801 2$bUk 801 2$bStDuBDSZ 801 2$bStDuBDSZ 906 $aBOOK 912 $a9910968095703321 996 $aA laboratory guide to the mammalian embryo$94463175 997 $aUNINA