LEADER 03543nam 2200577 a 450 001 9910966502303321 005 20251117005958.0 010 $a1-61728-560-9 035 $a(CKB)2670000000041927 035 $a(EBL)3020859 035 $a(SSID)ssj0000425141 035 $a(PQKBManifestationID)12109329 035 $a(PQKBTitleCode)TC0000425141 035 $a(PQKBWorkID)10475334 035 $a(PQKB)10124364 035 $a(MiAaPQ)EBC3020859 035 $a(Au-PeEL)EBL3020859 035 $a(CaPaEBR)ebr10680997 035 $a(OCoLC)662456733 035 $a(BIP)33698119 035 $a(BIP)27327231 035 $a(EXLCZ)992670000000041927 100 $a20090622d2009 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aPerspectives on lipase enzyme technology /$fJ. Geraldine Sandana Mala and Satoru Takeuchi 205 $a1st ed. 210 $aNew York $cNova Science Publishers, Inc.$dc2009 215 $a1 online resource (161 p.) 225 1 $aBiotechnology in agriculture, industry and medicine series 300 $a"Nova biomedical"--cover. 311 08$a1-60741-977-7 320 $aIncludes bibliographical references (p. [135]-137) and index. 327 $aBiotechnology : the science and origin of lipase -- Isolation strategies of microbial lipases -- Lipase production : defining the media -- Lipase purification and characterization -- Lipase structure -- Molecular cloning and overexpression -- Bioinformatics -- Lipases : biocatalysts for the future. 330 $aLipases are triacylglycerol acylhydrolases that catalyse the hydrolysis of triglycerides to release concomitant free fatty acids and glycerol. Lipases are versatile enzymes with multiplexity of catalysis and therefore, constitute an important class of industrial enzymes of recent interests in basic and applied research. This book serves as a handbook for researchers in the Life Sciences and particularly for those who plan to investigate microbial lipases. It is also aimed to provide an updated and comprehensive review of laboratory activities on lipases, in particular from the microbial sources, thereupon, to impart good scientific perceptions to the reader in this discipline. The production characteristics are fundamental for manipulation of maximal enzyme titres. Considerable importance is also necessitated to gain genetically modified strains to enable enormous availability of the recombinant lipases for structural elucidations. Detailed descriptions on the isolation methods, production requirements, protein investigations and molecular cloning of the microbial lipases are presented. Bioinformatics approaches are also specifically discussed. Furthermore, lipase as a biocatalyst candidate is specifically included to target the application prospects of the lipase. Experimental protocols are presented for bench work practice. An appendix section is supplemented with significant data for the researcher as a reference tool for their routine laboratory investigations. 410 0$aBiotechnology in agriculture, industry and medicine series. 606 $aLipase$xBiotechnology 615 0$aLipase$xBiotechnology. 676 $a660.6/34 700 $aMala$b J. Geraldine Sandana$01861031 701 $aTakeuchi$b Satoru$01861032 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910966502303321 996 $aPerspectives on lipase enzyme technology$94467047 997 $aUNINA