LEADER 05311nam 22006614a 450 001 9910963082003321 005 20251116150308.0 010 $a1-280-36794-6 010 $a9786610367948 010 $a0-470-23229-3 010 $a0-471-62386-5 010 $a0-471-62362-8 035 $a(CKB)111087027142166 035 $a(EBL)468856 035 $a(OCoLC)54711493 035 $a(SSID)ssj0000111573 035 $a(PQKBManifestationID)11145479 035 $a(PQKBTitleCode)TC0000111573 035 $a(PQKBWorkID)10080713 035 $a(PQKB)11551225 035 $a(DNLM)101185860 035 $a(MiAaPQ)EBC468856 035 $a(Au-PeEL)EBL468856 035 $a(CaPaEBR)ebr10114042 035 $a(CaONFJC)MIL36794 035 $a(EXLCZ)99111087027142166 100 $a20030723d2004 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aBioanalytical chemistry /$fSusan R. Mikkelsen, Eduardo Corton 205 $a1st ed. 210 $aHoboken, N.J. $cJohn Wiley & Sons$dc2004 215 $a1 online resource (381 p.) 300 $aDescription based upon print version of record. 311 08$a0-471-54447-7 320 $aIncludes bibliographical references and index. 327 $aBIOANALYTICAL CHEMISTRY; CONTENTS; Preface; Acknowledgments; 1. Spectroscopic Methods for Matrix Characterization; 1.1 Introduction; 1.2 Total Protein; 1.2.1 Lowry Method; 1.2.2 Smith (BCA) Method; 1.2.3 Bradford Method; 1.2.4 Ninhydrin-Based Assay; 1.2.5 Other Protein Quantitation Methods; 1.3 Total DNA; 1.3.1 Diaminobenzoic Acid Method; 1.3.2 Diphenylamine Method; 1.3.3 Other Fluorometric Methods; 1.4 Total RNA; 1.5 Total Carbohydrate; 1.5.1 Ferricyanide Method; 1.5.2 Phenol-Sulfuric Acid Method; 1.5.3 2-Aminothiophenol Method; 1.5.4 Purpald Assay for Bacterial Polysaccharides 327 $a1.6 Free Fatty AcidsReferences; Problems; 2. Enzymes; 2.1 Introduction; 2.2 Enzyme Nomenclature; 2.3 Enzyme Commission Numbers; 2.4 Enzymes in Bioanalytical Chemistry; 2.5 Enzyme Kinetics; 2.5.1 Simple One-Substrate Enzyme Kinetics; 2.5.2 Experimental Determination of Michaelis-Menten Parameters; 2.5.2.1 Eadie-Hofstee Method; 2.5.2.2 Hanes Method; 2.5.2.3 Lineweaver-Burk Method; 2.5.2.4 Cornish-Bowden-Eisenthal Method; 2.5.3 Comparison of Methods for the Determination of K(m) Values; 2.5.4 One-Substrate, Two-Product Enzyme Kinetics; 2.5.5 Two-Substrate Enzyme Kinetics 327 $a2.5.6 Examples of Enzyme-Catalyzed Reactions and Their Treatment2.6 Enzyme Activators; 2.7 Enzyme Inhibitors; 2.7.1 Competitive Inhibition; 2.7.2 Noncompetitive Inhibition; 2.7.3 Uncompetitive Inhibition; 2.8 Enzyme Units and Concentrations; Suggested References; References; Problems; 3. Quantitation of Enzymes and Their Substrates; 3.1 Introduction; 3.2 Substrate Depletion or Product Accumulation; 3.3 Direct and Coupled Measurements; 3.4 Classification of Methods; 3.5 Instrumental Methods; 3.5.1 Optical Detection; 3.5.1.1 Absorbance; 3.5.1.2 Fluorescence; 3.5.1.3 Luminescence 327 $a3.5.1.4 Nephelometry3.5.2 Electrochemical Detection; 3.5.2.1 Amperometry; 3.5.2.2 Potentiometry; 3.5.2.3 Conductimetry; 3.5.3 Other Detection Methods; 3.5.3.1 Radiochemical; 3.5.3.2 Manometry; 3.5.3.3 Calorimetry; 3.6 Ultra-High-Throughput Assays (HTA); 3.7 Practical Considerations for Enzymatic Assays; Suggested References; References; Problems; 4. Immobilized Enzymes; 4.1 Introduction; 4.2 Immobilization Methods; 4.2.1 Nonpolymerizing Covalent Immobilization; 4.2.1.1 Controlled-Pore Glass; 4.2.1.2 Polysaccharides; 4.2.1.3 Polyacrylamide; 4.2.1.4 Acidic Supports; 4.2.1.5 Anhydride Groups 327 $a4.2.1.6 Thiol Groups4.2.2 Cross-Linking with Bifunctional Reagents; 4.2.3 Adsorption; 4.2.4 Entrapment; 4.2.5 Microencapsulation; 4.3 Properties of Immobilized Enzymes; 4.4 Immobilized Enzyme Reactors; 4.5 Theoretical Treatment of Packed-Bed Enzyme Reactors; Suggested References; References; Problems; 5. Antibodies; 5.1 Introduction; 5.2 Structural and Functional Properties of Antibodies; 5.3 Polyclonal and Monoclonal Antibodies; 5.4 Antibody-Antigen Interactions; 5.5 Analytical Applications of Secondary Antibody-Antigen Interactions; 5.5.1 Agglutination Reactions 327 $a5.5.2 Precipitation Reactions 330 $aBioanalytical Chemistry provides a thorough introduction for students and practitioners with a broad range of backgrounds from chemistry to medicine. In so doing, it brings together many of the techniques commonly used by biochemists and molecular biologists. The text includes entire chapters on design and implementation of enzyme assays; mass spectrometry; and validation of new methods. Each chapter progresses from basic concepts to applications involving real samples, and ends with a set of problems, while an appendix contains selected answers. The authors have limited mathematical derivatio 606 $aAnalytical biochemistry 615 0$aAnalytical biochemistry. 676 $a572/.36 700 $aMikkelsen$b Susan R.$f1960-$01360558 701 $aCorto?n$b Eduardo$f1962-$01360559 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910963082003321 996 $aBioanalytical chemistry$93378058 997 $aUNINA