LEADER 04898nam 22008535 450 001 9910962136103321 005 20250729110519.0 010 $a0-306-47109-4 024 7 $a10.1007/b111467 035 $a(CKB)111056485440758 035 $a(SSID)ssj0000140078 035 $a(PQKBManifestationID)11134791 035 $a(PQKBTitleCode)TC0000140078 035 $a(PQKBWorkID)10029276 035 $a(PQKB)11709220 035 $a(DE-He213)978-0-306-47109-4 035 $a(Au-PeEL)EBL3035678 035 $a(CaPaEBR)ebr10052993 035 $a(OCoLC)923696438 035 $a(Au-PeEL)EBL5584456 035 $a(OCoLC)1066190242 035 $a(MiAaPQ)EBC3035678 035 $a(EXLCZ)99111056485440758 100 $a20100301d2000 u| 0 101 0 $aeng 135 $aurnn|008mamaa 181 $ctxt 182 $cc 183 $acr 200 10$aDistance Measurements in Biological Systems by EPR /$fedited by Lawrence J. Berliner, Sandra S. Eaton, Gareth R. Eaton 205 $a1st ed. 2000. 210 1$aNew York, NY :$cSpringer US :$cImprint: Springer,$d2000. 215 $a1 online resource (XVIII, 614 p.) 225 1 $aBiological Magnetic Resonance,$x2512-2215 ;$v19 300 $aBibliographic Level Mode of Issuance: Monograph 311 08$a1-4757-0575-1 311 08$a0-306-46533-7 320 $aIncludes bibliographical references and index. 327 $aDistance Measurements by CW and Pulsed EPR -- Relaxation Times of Organic Radicals and Transition Metal Ions -- Structural Information from CW-EPR Spectra of Dipolar Coupled Nitroxide Spin Labels -- Determination of Protein Folds and Conformational Dynamics Using Spin-Labeling EPR Spectroscopy -- EPR Spectroscopic Ruler: the Deconvolution Method and its Applications -- TOAC -- Depth of Immersion of Paramagnetic Centers in Biological Systems -- Determination of Distances Based on T1 and Tm Effects -- Double-Quantum ESR and Distance Measurements -- ?2+1? Pulse Sequence as Applied for Distance and Spatial Distribution Measurements of Paramagnetic Centers -- Double Electron-Electron Resonance -- Electron Paramagnetic Resonance Distance Measurements in Photosystems -- Photo-Induced Radical Pairs Investigated Using Out-of-Phase Electron Spin Echo. 330 $aDistance measurements in biological systems by EPR The foundation for understanding function and dynamics of biological systems is knowledge of their structure. Many experimental methodologies are used for determination of structure, each with special utility. Volumes in this series on Biological Magnetic Resonance emphasize the methods that involve magnetic resonance. This volume seeks to provide a critical evaluation of EPR methods for determining the distances between two unpaired electrons. The editors invited the authors to make this a very practical book, with specific numerical examples of how experimental data is worked up to produce a distance estimate, and realistic assessments of uncertainties and of the range of applicability, along with examples of the power of the technique to answer biological problems. The first chapter is an overview, by two of the editors, of EPR methods to determine distances, with a focus on the range of applicability. The next chapter, also by the Batons, reviews what is known about electron spin relaxation times that are needed in estimating distances between spins or in selecting appropriate temperatures for particular experiments. Albert Beth and Eric Hustedt describe the information about spin-spin interaction that one can obtain by simulating CW EPR line shapes of nitroxyl radicals. The information in fluid solution CW EPR spectra of dual-spin labeled proteins is illustrated by Hassane Mchaourab and Eduardo Perozo. 410 0$aBiological Magnetic Resonance,$x2512-2215 ;$v19 606 $aRadiology 606 $aBiochemistry 606 $aBiophysics 606 $aAtoms 606 $aMolecules 606 $aChemistry, Physical and theoretical 606 $aRadiology 606 $aBiochemistry 606 $aBiophysics 606 $aAtomic, Molecular and Chemical Physics 606 $aPhysical Chemistry 615 0$aRadiology. 615 0$aBiochemistry. 615 0$aBiophysics. 615 0$aAtoms. 615 0$aMolecules. 615 0$aChemistry, Physical and theoretical. 615 14$aRadiology. 615 24$aBiochemistry. 615 24$aBiophysics. 615 24$aAtomic, Molecular and Chemical Physics. 615 24$aPhysical Chemistry. 676 $a538.364 701 $aBerliner$b Lawrence J$0745975 701 $aEaton$b Gareth R$093506 701 $aEaton$b Sandra S$01646851 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910962136103321 996 $aDistance measurements in biological systems by EPR$94387957 997 $aUNINA