LEADER 05531nam 2200721Ia 450 001 9910959261203321 005 20241018114924.0 010 $a9783527668625 010 $a3527668624 010 $a9781283644105 010 $a128364410X 010 $a9783527668618 010 $a3527668616 035 $a(CKB)2670000000254018 035 $a(EBL)1031847 035 $a(OCoLC)811963710 035 $a(SSID)ssj0000722590 035 $a(PQKBManifestationID)12313404 035 $a(PQKBTitleCode)TC0000722590 035 $a(PQKBWorkID)10698829 035 $a(PQKB)10872894 035 $a(MiAaPQ)EBC1031847 035 $a(Au-PeEL)EBL1031847 035 $a(CaPaEBR)ebr10605313 035 $a(CaONFJC)MIL395660 035 $a(Perlego)1002682 035 $a(EXLCZ)992670000000254018 100 $a20120813d2012 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aEpigenetic regulation and epigenomics /$fedited by Robert A. Meyers 205 $a1st ed. 210 $aWeinheim $cWiley-Blackwell$dc2012 215 $a1 online resource (1239 p.) 225 0 $aAdvances in molecular biology and medicine 300 $aDescription based upon print version of record. 311 08$a9783527326822 311 08$a3527326820 320 $aIncludes bibliographical references and index. 327 $aEpigenetic Regulation and Epigenomics; Contents; Preface and Commentary; List of Contributors; Part I Analytical Methods; 1 RNA Methodologies; 1 Introduction; 2 Subpopulations of RNA; 3 Goals in the Purification of RNA; 4 Methods of Cellular Disruption and RNA Recovery; 5 Inhibition of Ribonuclease Activity; 6 Methods for the Analysis of RNA; 7 Summary; References; 2 All Things ChIP: ChIP-Chip, ChIP-Seq, ChIP-PCR; 1 Introduction; 2 Protein-DNA Binding; 3 ChIP Protocol; 4 ChIP-PCR, ChIP-Chip, or ChIP-Seq. Which Should be Chosen?; 5 Experimental Considerations; 6 Calculating IP Enrichment 327 $a7 Special Analysis Considerations8 Conclusions; References; 3 Methods for DNA Methylation Analysis; 1 Introduction; 2 Methods of DNA Methylation Analysis; 3 Concluding Remarks; Acknowledgments; References; 4 DNA Methylation Analysis by MALDI Mass Spectrometry; Abbreviations; 1 Introduction to DNA Methylation; 2 Epigenetics and Disease; 3 DNA Methylation Content Analysis by Mass Spectrometry; 4 Specific DNA Methylation Analysis; 5 Introduction to MALDI; 6 Problems of MALDI Analysis of; 7 DNA Sequencing with MALDI Mass Spectrometry Readout 327 $a8 Primer Extension for the Interrogation of Specific Positions9 Quantification; 10 Automation of Sample Preparation, Data Accumulation, and Analysis; 11 Positioning of MALDI MS for DNA Methylation Analysis; 12 Applications of DNA Methylation Analysis by Mass Spectrometry; 13 DNA Methylation Analysis for Tumor Classification; 14 DNA Methylation Analysis for Prognosis and Response to Treatment; 15 Prenatal Diagnosis; 16 Conclusions; References; 5 Tag Sequencing; 1 Library Construction; 2 Advanced Sequencing Technologies; 3 Data Analysis and Bioinformatics; 4 Applications of Tag Sequencing 327 $a5 Clinical Applications of Tag Sequencing6 Future Perspectives; Acknowledgments; References; Part II Basic Molecular Mechanisms; 6 Heterochromatin and Euchromatin - Organization, Boundaries and Gene Regulation; 1 Properties and Functions of Heterochromatin; 2 Euchromatin Formation; 3 Boundaries between Heterochromatin and Euchromatin; 4 Insulating against Active Chromatin; Acknowledgments; References; 7 Regulation of Gene Expression; 1 Introduction; 2 Regulation of Gene Expression in Prokaryotes; 3 Regulation of Gene Expression in Eukaryotes; 4 RNA Splicing 327 $a5 Role of microRNAs (miRNAs) in the Regulation of Gene Expression6 Chromatin Structure and the Control of Gene Expression; 7 Epigenetic Control of Gene Expression; 8 Gene Regulation by Hormonal Action; 9 Post-Transcriptional Regulation of mRNA; 10 Transport of Processed mRNA to the Cytoplasm; 11 Regulation of Gene Expression at the Level of Translation; Acknowledgments; References; 8 Molecular Genetics of Genomic Imprinting; 1 Genomic Imprinting in Mammals; 2 Imprinted Genes; 3 Molecular Mechanisms; 4 Imprinting and Disease; 5 Human Reproduction; 6 Evolution of Imprinting; References 327 $a9 Nuclear Transfer for Cloning Animals 330 $aEpigenetics is a term in biology referring to heritable traits that do not involve changes in the underlying DNA sequence of the organism. Epigenetic traits exist on top of or in addition to the traditional molecular basis for inheritance. The ""epigenome"" is a parallel to the word ""genome,"" and refers to the overall epigenetic state of a cell. Cancer and stem cell research have gradually focused attention on these genome modifications. The molecular basis of epigenetics involves modifications to DNA and the chromatin proteins that associate with it. Methylation, for example, can silence a 410 0$aCurrent Topics from the Encyclopedia of Molecular Cell Biology and Molecular Medicine 606 $aGenetic regulation 606 $aEpigenesis 615 0$aGenetic regulation. 615 0$aEpigenesis. 676 $a572.865 701 $aMeyers$b Robert A$g(Robert Allen),$f1936-$08008 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910959261203321 996 $aEpigenetic regulation and epigenomics$94358416 997 $aUNINA