LEADER 05453nam 22006854a 450 001 9910959060003321 005 20200520144314.0 010 $a1-281-27935-8 010 $a9786611279356 010 $a0-08-055854-2 035 $a(CKB)1000000000398474 035 $a(EBL)340642 035 $a(OCoLC)437208903 035 $a(SSID)ssj0000202441 035 $a(PQKBManifestationID)12057040 035 $a(PQKBTitleCode)TC0000202441 035 $a(PQKBWorkID)10252780 035 $a(PQKB)10811123 035 $a(Au-PeEL)EBL340642 035 $a(CaPaEBR)ebr10225025 035 $a(CaONFJC)MIL127935 035 $a(CaSebORM)9780080558547 035 $a(MiAaPQ)EBC340642 035 $a(PPN)143422316 035 $a(EXLCZ)991000000000398474 100 $a20071218d2008 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aMicroscope image processing /$f[edited by] Qiang Wu, Fatima A. Merchant, Kenneth R. Castleman 205 $a1st edition 210 $aAmsterdam ;$aBoston $cElsevier/Academic Press$dc2008 215 $a1 online resource (585 p.) 300 $aDescription based upon print version of record. 311 08$a0-12-372578-X 320 $aIncludes bibliographical references and index. 327 $aFront Cover; Microscope Image Processing; Copyright Page; Contents; Foreword; Reference; Preface; Acknowledgments; Chapter 1: Introduction; 1.1 The Microscope and Image Processing; 1.2 Scope of This Book; 1.3 Our Approach; 1.3.1 The Four Types of Images; 1.3.1.1 Optical Image; 1.3.1.2 Continuous Image; 1.3.1.3 Digital Image; 1.3.1.4 Displayed Image; 1.3.2 The Result; 1.3.2.1 Analytic Functions; 1.3.3 The Sampling Theorem; 1.4 The Challenge; 1.5 Nomenclature; 1.6 Summary of Important Points; References; Chapter 2: Fundamentals of Microscopy; 2.1 Origins of the Microscope; 2.2 Optical Imaging 327 $a2.2.1 Image Formation by a Lens2.2.1.1 Imaging a Point Source; 2.2.1.2 Focal Length; 2.2.1.3 Numerical Aperture; 2.2.1.4 Lens Shape; 2.3 Diffraction-Limited Optical Systems; 2.3.1 Linear System Analysis; 2.4 Incoherent Illumination; 2.4.1 The Point Spread Function; 2.4.2 The Optical Transfer Function; 2.5 Coherent Illumination; 2.5.1 The Coherent Point Spread Function; 2.5.2 The Coherent Optical Transfer Function; 2.6 Resolution; 2.6.1 Abbe Distance; 2.6.2 Rayleigh Distance; 2.6.3 Size Calculations; 2.7 Aberration; 2.8 Calibration; 2.8.1 Spatial Calibration; 2.8.2 Photometric Calibration 327 $a2.9 Summary of Important PointsReferences; Chapter 3: Image Digitization; 3.1 Introduction; 3.2 Resolution; 3.3 Sampling; 3.3.1 Interpolation; 3.3.2 Aliasing; 3.4 Noise; 3.5 Shading; 3.6 Photometry; 3.7 Geometric Distortion; 3.8 Complete System Design; 3.8.1 Cumulative Resolution; 3.8.2 Design Rules of Thumb; 3.8.2.1 Pixel Spacing; 3.8.2.2 Resolution; 3.8.2.3 Noise; 3.8.2.4 Photometry; 3.8.2.5 Distortion; 3.9 Summary of Important Points; References; Chapter 4: Image Display; 4.1 Introduction; 4.2 Display Characteristics; 4.2.1 Displayed Image Size; 4.2.2 Aspect Ratio 327 $a4.2.3 Photometric Resolution4.2.4 Grayscale Linearity; 4.2.5 Low-Frequency Response; 4.2.5.1 Pixel Polarity; 4.2.5.2 Pixel Interaction; 4.2.6 High-Frequency Response; 4.2.7 The Spot-Spacing Compromise; 4.2.8 Noise Considerations; 4.3 Volatile Displays; 4.4 Sampling for Display Purposes; 4.4.1 Oversampling; 4.4.2 Resampling; 4.5 Display Calibration; 4.6 Summary of Important Points; References; Chapter 5: Geometric Transformations; 5.1 Introduction; 5.2 Implementation; 5.3 Gray-Level Interpolation; 5.3.1 Nearest-Neighbor Interpolation; 5.3.2 Bilinear Interpolation; 5.3.3 Bicubic Interpolation 327 $a5.3.4 Higher-Order Interpolation5.4 Spatial Transformation; 5.4.1 Control-Grid Mapping; 5.5 Applications; 5.5.1 Distortion Removal; 5.5.2 Image Registration; 5.5.3 Stitching; 5.6 Summary of Important Points; References; Chapter 6: Image Enhancement; 6.1 Introduction; 6.2 Spatial Domain Methods; 6.2.1 Contrast Stretching; 6.2.2 Clipping and Thresholding; 6.2.3 Image Subtraction and Averaging; 6.2.4 Histogram Equalization; 6.2.5 Histogram Specification; 6.2.6 Spatial Filtering; 6.2.7 Directional and Steerable Filtering; 6.2.8 Median Filtering; 6.3 Fourier Transform Methods 327 $a6.3.1 Wiener Filtering and Wiener Deconvolution 330 $aDigital image processing, an integral part of microscopy, is increasingly important to the fields of medicine and scientific research. This book provides a unique one-stop reference on the theory, technique, and applications of this technology.Written by leading experts in the field, this book presents a unique practical perspective of state-of-the-art microscope image processing and the development of specialized algorithms. It contains in-depth analysis of methods coupled with the results of specific real-world experiments. Microscope Image Processing covers image digitization and di 606 $aMicroscopy 606 $aMicroscopes 615 0$aMicroscopy. 615 0$aMicroscopes. 676 $a502.82 701 $aWu$b Qiang$f1958-$0318232 701 $aMerchant$b Fatima Aziz$f1967-$0318233 701 $aCastleman$b Kenneth R$021396 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910959060003321 996 $aMicroscope Image Processing$9806759 997 $aUNINA