LEADER 03939nam 2200661 a 450 001 9910781496103321 005 20230725050635.0 010 $a0-8047-7677-6 024 7 $a10.1515/9780804776776 035 $a(CKB)2550000000040517 035 $a(EBL)730440 035 $a(OCoLC)742361663 035 $a(SSID)ssj0000521877 035 $a(PQKBManifestationID)12250882 035 $a(PQKBTitleCode)TC0000521877 035 $a(PQKBWorkID)10527416 035 $a(PQKB)11441146 035 $a(MiAaPQ)EBC730440 035 $a(DE-B1597)564793 035 $a(DE-B1597)9780804776776 035 $a(Au-PeEL)EBL730440 035 $a(CaPaEBR)ebr10483645 035 $a(OCoLC)1178769778 035 $a(EXLCZ)992550000000040517 100 $a20100524d2011 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aDark logic$b[electronic resource] $etransnational criminal tactics and global security /$fRobert Mandel 210 $aStanford, Calif. $cStanford Security Studies$d2011 215 $a1 online resource (268 p.) 300 $aDescription based upon print version of record. 311 $a0-8047-6992-3 311 $a0-8047-6993-1 320 $aIncludes bibliographical references (p. 229-250) and index. 327 $aIntroduction : the central question -- Transnational organized crime's changing threat -- Corruption/violence and individual/state security -- Major transnational criminal organizations -- Major transnational criminal activities -- Analysis of case patterns -- Links between transnational criminals and terrorists -- Conclusion : policy implications. 330 $aSince the end of the Cold War, transnational non-state forces have been a major source of global instability, with many ominous and disruptive flows of people, goods, and services moving readily across international boundaries. And because these activities are so multifaceted and so intertwined within the fabric of society, they remain largely invisible until the intrusion is well-advanced and difficult to reverse. Thus, the threat posed by transnational organized crime ultimately undermines the total security of countries?including the economic, cultural, and political dimensions?and now presents an international security challenge of staggering proportions. Surprisingly, no single book so far has fully addressed the scale of this threat to global stability from an international security perspective. In an attempt to rectify that failure, Dark Logic examines in depth when and how transnational organized crime is likely to use corruption and violence to achieve its ends, and when and how these criminal activities most affect individual and state security. Even more important, it pinpoints when and how the negative consequences of these tactics and activities can be most successfully combated. In so doing it provides a unique lens for analyzing today's global security dilemmas. Given that the threat associated with transnational organized crime can endanger all citizens?from policy makers and security analysts to students, scholars, and the "man and woman on the street"?this book is written in an intelligible and jargon-free style to make it accessible to anyone interested in the ever-growing catalog of threats to national and international security. 606 $aTransnational crime 606 $aOrganized crime 606 $aCorruption 606 $aViolence 606 $aSecurity, International 615 0$aTransnational crime. 615 0$aOrganized crime. 615 0$aCorruption. 615 0$aViolence. 615 0$aSecurity, International. 676 $a364.1/35 700 $aMandel$b Robert$f1949-$01108223 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910781496103321 996 $aDark logic$93792153 997 $aUNINA LEADER 04235nam 2200709Ia 450 001 9910957092703321 005 20251117091742.0 010 $a9786613310972 010 $a9781283310970 010 $a128331097X 010 $a9780123855459 010 $a0123855454 035 $a(CKB)2550000000063471 035 $a(EBL)802466 035 $a(OCoLC)769342081 035 $a(SSID)ssj0000591978 035 $a(PQKBManifestationID)11352048 035 $a(PQKBTitleCode)TC0000591978 035 $a(PQKBWorkID)10728666 035 $a(PQKB)11568203 035 $a(Au-PeEL)EBL802466 035 $a(CaPaEBR)ebr10509200 035 $a(CaONFJC)MIL331097 035 $z(PPN)182565076 035 $a(MiAaPQ)EBC802466 035 $a(PPN)176592040 035 $a(EXLCZ)992550000000063471 100 $a20111109d2012 uy 0 101 0 $aeng 135 $aurcn||||||||| 181 $ctxt$2rdacontent 181 $csti$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aMolecular biology techniques $ea classroom laboratory manual /$fSusan Carson, Heather Miller, D. Scott Witherow 205 $a3rd ed. 210 $aAmsterdam ;$aBoston $cElsevier /Academic Press$dc2012 215 $a1 online resource (xxvi, 200 pages) $ccolor illustrations 300 $aIncludes index. 300 $aPrevious edition: 2006. 311 08$a9780123855442 311 08$a0123855446 320 $aIncludes bibliographical references and index. 327 $aLab Session 1 Getting Oriented: Practicing with Micropipettes -- Lab Session 2 Purification and Digestion of Plasmid (Vector) DNA -- Lab Session 3 PCR Amplification of egfp and Completion of Vector Preparation -- Lab Session 4 Preparation of Insert DNA (egfp) PCR Product -- Lab Session 5 DNA Ligation and Transformation of Escherichia coli -- Lab Session 6 Colony Hybridization -- Lab Session 7 Characterization of Recombinant Clones: Part 1 -- Lab Session 8 Characterization of Recombinant Clones: Part 2 -- Lab Session 9 Characterization of Recombinant Clones: Part 3 -- Lab Session 10 Computational Analysis of DNA Sequence from a Positive Clone: Part 2 -- Lab Session 11 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 1 -- Lab Session 12 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 2 -- Lab Session 13 Extraction of Recombinant Protein from Escherichia coli Using a Glutathione Affinity Column -- Lab Session 14 Analysis of Purification Fractions -- Lab Session 15 Total RNA Purification -- Lab Session 16 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 1 -- Lab Session 17 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 2 -- Lab Session 18 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 1 -- Lab Session 19 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 2 -- Appendix 1 Equipment -- Appendix 2 Prep List -- Appendix 3 Preparation of Competent E. coli Cells -- Appendix 4 Pre-Lab Questions. 330 $aThis manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein.The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, 606 $aMolecular biology$vLaboratory manuals 606 $aMolecular biology$xTechnique$vHandmooks, manuals, etc 615 0$aMolecular biology 615 0$aMolecular biology$xTechnique 676 $a572.8/078 676 $a572.8078 676 $a572.8078 700 $aCarson$b Sue$g(Susan)$01860928 701 $aMiller$b Heather B$01860929 701 $aWitherow$b D. Scott$01860930 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910957092703321 996 $aMolecular biology techniques$94466888 997 $aUNINA