LEADER 04460oam 2200637Ia 450 001 9910956367103321 005 20250512162432.0 010 $a9786613885111 010 $a9780123948335$bebook 010 $a0123948339$bebook 010 $a9781283572668 010 $a1283572664 035 $a(CKB)2670000000233489 035 $a(EBL)1001358 035 $a(OCoLC)810414726 035 $a(SSID)ssj0000737444 035 $a(PQKBManifestationID)11378476 035 $a(PQKBTitleCode)TC0000737444 035 $a(PQKBWorkID)10786598 035 $a(PQKB)10252491 035 $a(Au-PeEL)EBL1001358 035 $a(CaPaEBR)ebr10593898 035 $a(CaONFJC)MIL388511 035 $a(MiAaPQ)EBC1001358 035 $a(EXLCZ)992670000000233489 100 $a20111102d2013 uy 0 101 0 $aeng 135 $aurcn#---auuuu 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aForensic DNA biology $ea laboratory manual /$fKelly M. Elkins 205 $a1st ed. 210 1$aOxford, UK ;$aWaltham, MA :$cAcademic Press, an imprint of Elsevier,$d2013. 215 $a1 online resource (225 pages) $cillustrations 300 $aIncludes bibliographical references and index. 311 0 $a9780123945853 311 0 $a0123945852 327 $aFront Cover; Forensic DNA Biology: A Laboratory Manual; Copyright; Contents; Acknowledgements; About the Author; Welcome; Forensic DNA Biology: An Introduction; Laboratory Safety; Avoiding Contamination Issues: Standard Laboratory Practices; Chapter 1 - Pipetting; Chapter 2 - Serology; Chapter 3 - Sampling Biological Evidence for DNA Extraction; Chapter 4 - DNA Extraction; Chapter 5 - Determination of Quality and Quantity of DNA Using Agarose Gel Electrophoresis; Chapter 6 - Determination of DNA Quality and Quantity Using UV-Vis Spectroscopy; 327 $aChapter 7 - Determination of DNA Quantity by Fluorescence Spectroscopy; Chapter 8 - Real-Time Polymerase Chain Reaction (PCR) Quantitation of DNA; Chapter 9 - Multiplex Polymerase Chain Reaction (PCR) Primer Design (in Silico); Chapter 10 - Testing Designed Polymerase Chain Reaction (PCR) Primers in Multiplex Reactions; Chapter 11 - Multiplex Polymerase Chain Reaction (PCR) Amplification of Short Tandem Repeat (STR) Loci Using a Commercial Kit; Chapter 12 - Capillary Electrophoresis of Short Tandem Repeat (STR) Polymerase Chain Reaction (PCR) Products from a Commercial Multiplex Kit; 327 $aChapter 13 - Computing Random Match Probability from DNA Profile Data Using Population Databases; Chapter 14 - Mitochondrial Deoxyribonucleic Acid (mtDNA) Single Nucleotide Polymorphism (SNP) Detection; Chapter 15 - Analysis of Deoxyribonucleic Acid (DNA) Sequence Data Using BioEdit; Chapter 16 - Ribonucleic Acid (RNA) Extraction; Chapter 17 - Y-STR Polymerase Chain Reaction (PCR) Deoxyribonucleic acid (DNA) Amplification and Typing; Chapter 18 - Human Genetic Analysis: Paternity or Missing Persons Cases and Statistics; Chapter 19 - Low Copy Number Stochastic Results; Chapter 20 - Using in Silico Methods to Construct a Short-Tandem Repeat (STR) Deoxyribonucleic Acid (DNA) Sequence for Cloning; Chapter 21 - Deoxyribonucleic Acid (DNA) Extraction from Botanical Material and Polymerase Chain Reaction (PCR) Amplification; Chapter 22 - Social, Ethical, and Regulatory Concerns; Selected Forensic DNA Biology Case Studies; Index. 330 $aDNA typing has revolutionized criminal investigations and has become a powerful tool in the identification of individuals in criminal and paternity cases. Forensic DNA Biology: A Laboratory Manual is comprised of up-to-date and practical experiments and step-by-step instructions on how to perform DNA analysis, including pipetting, microscopy and hair analysis, presumptive testing of body fluids and human DNA typing. Modern DNA typing techniques are provided, reflecting real life, where not all institutions and crime labs can afford the same equipment and software. Real case studies w 606 $aForensic genetics$xTechnique 606 $aDNA fingerprinting 615 0$aForensic genetics$xTechnique. 615 0$aDNA fingerprinting. 676 $a572.86 676 $a614.1 700 $aElkins$b Kelly M.$01592672 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910956367103321 996 $aForensic DNA biology$94371399 997 $aUNINA