LEADER 02733oam 2200421zu 450 001 9910830631803321 005 20210807002928.0 010 $a3-527-60978-4 035 $a(CKB)1000000000376876 035 $a(SSID)ssj0000354544 035 $a(PQKBManifestationID)11965139 035 $a(PQKBTitleCode)TC0000354544 035 $a(PQKBWorkID)10302558 035 $a(PQKB)10205032 035 $a(NjHacI)991000000000376876 035 $a(EXLCZ)991000000000376876 100 $a20160829d1999 uy 101 0 $ager 135 $aur||||||||||| 181 $ctxt 182 $cc 183 $acr 200 10$aNordost-Afrika - Strukturen and Ressourcen 210 31$a[Place of publication not identified]$cWiley VCH Imprint$d1999 215 $a1 online resource (xxii, 676 pages) $cillustrations 300 $aBibliographic Level Mode of Issuance: Monograph 311 $a3-527-27724-2 327 $aZiele, Probleme und Ergebnise des Sonderforschungsbereichs - Darstellung eines nicht alltaglichen Gro?projekts aus der Sicht des Sprechers Der strukturelle Rahmen Nordost-Afrikas am Ende der panafrikanischen Orogenese Beckenentwicklung und Sedimentationsprozesse in kratonalen Bereichen Nordost-Afrikas im Phanerozoikum Junger Vulkanismus, Mantle Plumes und Tektonik im Sudan Mesozoische Floren aus Nordost-Afrika und ihre Beziehungen zum Klima am Palaoaquator Kontinentale Wirbeltierfaunen aus der Oberkreide des Nordsudan Geophysikalische und geologische Untersuchungen im Es Safya-Graben (Nordost-Sudan): Ein Beitrag zur Erkundung einer kleinraumigen Struktur Verwitterungsbedingte Lagerstattenbildung in Sedimentdecken Nordost-Afrikas Regionalgeologische Aspekte und geotektonische Kontrolle ophiolith-assoziierter Lagerstatten im nordost-sudanesischen Teilbereich des Arabisch-Nubischen Schilds Palao-Environment und Drainagesysteme der Ostsahara im Spatpleistozan und Holozan Terrestrische Palaoklimatologie heute arider Gebiete: Resultate aus dem Unteren Wadi Howar Sudostliche Sahara/Nordwest-Sudan Grundwasserressourcen im Nubischen Aquifersystem Quantitative Durrerisiko-Abschatzung fur agrare Raume in der Nordlichen Gezira (Sudan) Einsatz von Fernerkundung, digitaler Bildverarbeitung und Geo-Informationssystemen zur Gewinnung geologischer Informationen in Agypten und im Sudan Kartographie - Fernerkundung - Geo-Informationssysteme: Bearbeitung von raumbezogenen Informationen Dokumentation. 606 $aGeology$zAfrica, Northwest 615 0$aGeology 676 $a556.1 700 $aKlitzsch$01612550 702 $aThorweihe$b Ulf 801 0$bPQKB 906 $aBOOK 912 $a9910830631803321 996 $aNordost-Afrika - Strukturen and Ressourcen$93941408 997 $aUNINA LEADER 04356nam 2200589 a 450 001 9910954876203321 005 20251117070127.0 010 $a1-61324-220-4 035 $a(CKB)2670000000094932 035 $a(EBL)3019250 035 $a(SSID)ssj0000521468 035 $a(PQKBManifestationID)12175889 035 $a(PQKBTitleCode)TC0000521468 035 $a(PQKBWorkID)10523043 035 $a(PQKB)11436599 035 $a(MiAaPQ)EBC3019250 035 $a(Au-PeEL)EBL3019250 035 $a(CaPaEBR)ebr10670815 035 $a(OCoLC)923660450 035 $a(BIP)43288186 035 $a(BIP)28521754 035 $a(EXLCZ)992670000000094932 100 $a20091222d2010 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aChitosan hydrolysis by non-specific enzymes /$fWenshui Xia and Ping Liu 205 $a1st ed. 210 $aNew York $cNova Science Publishers$dc2010 215 $a1 online resource (78 p.) 225 1 $aEnvironmental Science, Engineering and Technology 300 $aDescription based upon print version of record. 311 08$a1-61668-152-7 320 $aIncludes bibliographical references and index. 327 $aIntro -- CHITOSAN HYDROLYSIS BY NON-SPECIFIC ENZYMES -- CHITOSAN HYDROLYSIS BY NON-SPECIFIC ENZYMES -- CONTENTS -- ABSTRACT -- Chapter 1 INTRODUCTION -- Chapter 2 CHARACTERIZATION OF CHITOSAN HYDROLYSIS BY NONSPECIFIC ENZYMES -- 2.1. CHARACTERIZATION OFCHITOSAN HYDROLYSIS BY NON-SPECIFIC CELLULASES -- (1) Temperature and pH -- (2) Molecular Weight -- (3) Reduction of the Viscosity -- (4) Kinetic Parameters -- (5) Metal Ions -- 2.2. CHARACTERIZATION OF CHITOSAN HYDROLYSIS BY NON-SPECIFIC LIPASE -- 2.3. Characterization of Chitosan Hydrolysis by Non-Specific Papain -- Chapter 3 MECHANISM OF NON-SPECIFIC ENZYMES TOWARD CHITOSAN -- 3.1. PURIFICATION AND CHARACTERIZATION OF CHITOSANOLYTIC COMPONENTS FROM NON -SPECIFIC ENZYMES -- 3.1.1. Purification and Characterization of a Bifunctional Enzyme with Chitosanolytic and Cellulolytic Activity from Commercial Cellulase -- 3.1.2. Purification and Characterization of a Bifunctional Chitosanase with Chitinase Activity from Commercial Lipase -- 3.2. ACTION MODE ANALYSIS OF BIFUNCTIONAL ENZYMES ON CHITOSAN -- 3.2.1. Substrate Specificity -- 3.2.2. Chitosan and Chitooligosaccharide Hydrolysis Analysis by CCBE -- 3.2.3. TLC Analysis of COS Hydrolysis Products by the Purified CNBE -- Chapter 4 STRUCTURE AND FUNCTION ANALYSIS OF BIFUNCTIONAL ENZYMES WITH CHITOSANOLYTIC ACTIVITY -- 4.1. AMINO ACID COMPOSITION OF PURIFIED CCBE AND CNBE -- 4.2. STRUCTURE ANALYSIS OF PURIFIED CCBE AND CNBE -- 4.2.1. N-Terminal Sequencing Analysis of CNBE -- 4.2.2. Sequencing Analysis of the Purified CCBE by MALDI-TOF Mass -- 4.3. DETERMINATION OF THE ACTIVE SITES OF CCBE AND CNBE BY CHEMICAL MODIFICATION -- 4.3.1. The Essential Carboxyl Group Modification using EDC -- 4.3.2. Modification of Tryptophan Residues using NBS -- 4.3.3. Modification of Histidine Residue using DEPC. 327 $a4.3.4. Modification of Tyrosine, Threonine/Serine, Arginine, Methionine Residues Etc -- Chapter 5 IDENTIFICATION OF CCBE FROM T.VIRIDE BY MOLECULAR CLONING -- 5.1. DETERMINATION OF FERMENTATION CONDITION OF T.VIRIDE -- 5.2. CLONING AND SEQUENCE ANALYSIS OF CCBE GENE -- 5.3. EXPRESSION ANALYSIS OF CCBE SPLICING GENES IN PICHIA PATORIS -- ACKNOWLEDGMENTS -- REFERENCE -- INDEX -- Blank Page. 330 $aThe focus of this book is the characterizations and hydrolyzing mechanism of the non-specific enzymes toward chitosan choosing the three typical non-specific enzymes: cellulase, lipase and papain as objects. The authors studied the enzymatic characteristics, purification, product analysis, glycoside bond cleavage, active sites and gene cloning of these enzymes to expatiate their non-specific hydrolysis mechanism. 410 0$aEnvironmental Science, Engineering and Technology 606 $aChitosan$xMetabolism 615 0$aChitosan$xMetabolism. 676 $a612.3/9 700 $aXia$b Wenshui$01870463 701 $aLiu$b Ping$01870464 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910954876203321 996 $aChitosan hydrolysis by non-specific enzymes$94478928 997 $aUNINA